Recombinant Mouse GDF-5 Protein

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse GDF-5 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.2‑1.2 µg/mL.
Source
E. coli-derived mouse GDF-5/BMP-14 protein
Ala376-Arg495, with and without an N-terminal Met
Accession #
N-terminal Sequence
Met and Ala376
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Gdf5
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
853-G5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 150 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse GDF-5 Protein

  • BMP14
  • BMP-14
  • Cartilage-derived morphogenetic protein 1
  • cartilage-derived morphogenetic protein-1
  • CDMP-1
  • CDMP1LAP4
  • GDF5
  • GDF-5
  • growth differentiation factor 5
  • growth/differentiation factor 5
  • OS5
  • Radotermin
  • SYNS2

Background

Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-­5 is synthesized as a large precursor protein that consists of an N­-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature mouse GDF-5 shares 99% aa sequence identity with both mature human and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
  3. Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
  4. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  5. Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
  6. Bragdon, B. et al. (2011) Cell. Signal. 23:609.
  7. Sieber, C. et al. (2006) Biol. Chem. 387:451.
  8. Osorio, C. et al. (2013) Development 140:4751.
  9. Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
  10. Francis-West, P.H. et al. (1999) Development 126:1305.
  11. Brunet, L.J. et al. (1998) Science 280:1455.
  12. Zeng, Q. et al. (2007) Bone 40:374.
  13. Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
  14. Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
  15. Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
  16. Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
  17. Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
  18. Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.

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Publications for GDF-5/BMP-14 (853-G5)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Bioassay, Flow, In Vivo, complex formation.


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Bioassay
(4)
Flow
(1)
In Vivo
(2)
complex formation
(1)
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Human
(2)
Mouse
(5)
Rat
(1)
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Showing Publications 1 - 8 of 8.
Publications using 853-G5 Applications Species
Y Zhao, M Zhang, H Liu, J Wang Signaling by growth/differentiation factor 5 through the bone morphogenetic protein receptor type IB protects neurons against kainic acid-induced neurodegeneration Neurosci. Lett., 2017;0(0):. 2017 [PMID: 28458020] (In Vivo, Mouse) In Vivo Mouse
Bethanie Imogen Ayerst GDF5 Mediated Enhancement of Chondrocyte Phenotype is Inhibited by Heparin; Implication for the use of Heparin in the Clinic and in Tissue Engineering Applications Tissue Eng Part A, 2016;0(0):. 2016 [PMID: 27899064] (Flow, Mouse) Flow Mouse
Craft A, Rockel J, Nartiss Y, Kandel R, Alman B, Keller G Generation of articular chondrocytes from human pluripotent stem cells. Nat Biotechnol, 2015;33(6):638-45. 2015 [PMID: 25961409] (Bioassay, Mouse) Bioassay Mouse
Ratnayake M, Ploger F, Santibanez-Koref M, Loughlin J Human chondrocytes respond discordantly to the protein encoded by the osteoarthritis susceptibility gene GDF5. PLoS ONE, 2014;9(1):e86590. 2014 [PMID: 24466161] (Bioassay, Human) Bioassay Human
Hinoi E, Nakamura Y, Takada S, Fujita H, Iezaki T, Hashizume S, Takahashi S, Odaka Y, Watanabe T, Yoneda Y Growth differentiation factor-5 promotes brown adipogenesis in systemic energy expenditure. Diabetes, 2013;63(1):162-75. 2013 [PMID: 24062245] (Bioassay, Mouse) Bioassay Mouse
Zaidi SH, Huang Q, Momen A, Riazi A, Husain M Growth differentiation factor 5 regulates cardiac repair after myocardial infarction. J. Am. Coll. Cardiol., 2010;55(2):135-43. 2010 [PMID: 20117381] (Bioassay, Mouse) Bioassay Mouse
Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY Targeting of bone morphogenetic protein growth factor complexes to fibrillin. J. Biol. Chem., 2008;283(20):13874-88. 2008 [PMID: 18339631] (complex formation, Human) complex formation Human
Shimaoka H, Dohi Y, Ohgushi H, Ikeuchi M, Okamoto M, Kudo A, Kirita T, Yonemasu K Recombinant growth/differentiation factor-5 (GDF-5) stimulates osteogenic differentiation of marrow mesenchymal stem cells in porous hydroxyapatite ceramic. J Biomed Mater Res A, 2004;68(1):168-76. 2004 [PMID: 14661262] (In Vivo, Rat) In Vivo Rat

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Bioinformatics

Gene Symbol Gdf5
Entrez
Uniprot