Recombinant Human BDNF Protein

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2 μg/lane of Recombinant Human BDNF was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 13-14 kDa and 11-12 kDa, respectively.
Recombinant Human BDNF (Catalog # 248-BDB) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 0.2‑2 ng/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, EqSpecies Glossary
Applications Binding Activity, Bioactivity

Order Details

Recombinant Human BDNF Protein Summary

Additional Information
100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined.
Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) is coated at 1 μg/mL, Recombinant Human BDNF binds with an apparent Kd <1 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived BDNF protein
His129-Arg247
Accession # P23560
100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined.
Accession #
N-terminal Sequence
His129
Protein/Peptide Type
Recombinant Proteins
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13-14 kDa, reducing conditions
Publications
Read Publications using
248-BDB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute 5 µg vials at 100 µg/mL in water. Reconstitute 10 µg or larger vials at 250 µg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BDNF Protein

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • neurotrophin

Background

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins (1, 2). All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor (3).

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

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Publications for BDNF (248-BDB)(123)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Porcine, Primate - Callitrix jacchus (Common Marmoset).

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance, Western Blot.


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Bioassay
(107)
Cell Culture
(1)
ELISA (Standard)
(1)
In Vivo
(11)
Surface Plasmon Resonance
(1)
Western Blot
(1)
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Human
(72)
Mouse
(32)
Rat
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Primate - Callitrix jacchus (Common Marmoset)
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All Species
Showing Publications 1 - 10 of 123. Show All 123 Publications.
Publications using 248-BDB Applications Species
T Ryynänen, A Pelkonen, K Grigoras, OME Ylivaara, T Hyvärinen, J Ahopelto, M Prunnila, S Narkilahti, J Lekkala Microelectrode Array With Transparent ALD TiN Electrodes Front Neurosci, 2019;13(0):226. 2019 [PMID: 30967754] (Bioassay, Human) Bioassay Human
B Labonté, YH Jeong, E Parise, O Issler, M Fatma, O Engmann, KA Cho, R Neve, EJ Nestler, JW Koo Gadd45b mediates depressive-like role through DNA demethylation Sci Rep, 2019;9(1):4615. 2019 [PMID: 30874581] (In Vivo, Mouse) In Vivo Mouse
N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson&#039;s disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019;9(1):4515. 2019 [PMID: 30872638] (Bioassay, Mouse) Bioassay Mouse
M Elkouris, G Kouroupi, A Vourvoukel, N Papagianna, V Kaltezioti, R Matsas, L Stefanis, M Xilouri, PK Politis Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson&#039;s Disease Patients Front Cell Neurosci, 2019;13(0):58. 2019 [PMID: 30853899] (Bioassay, Human) Bioassay Human
J Ooi, SR Langley, X Xu, KH Utami, B Sim, Y Huang, NP Harmston, YL Tay, A Ziaei, R Zeng, D Low, F Aminkeng, RM Sobota, F Ginhoux, E Petretto, MA Pouladi Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects Cell Rep, 2019;26(9):2494-2508.e7. 2019 [PMID: 30811996] (Bioassay, Human) Bioassay Human
L Wang, AJ De Solis, Y Goffer, KE Birkenbach, SE Engle, R Tanis, JM Levenson, X Li, R Rausch, M Purohit, JY Lee, J Tan, MC De Rosa, CA Doege, HL Aaron, GJ Martins, JC Brüning, D Egli, R Costa, N Berbari, RL Leibel, G Stratigopo Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development JCI Insight, 2019;4(3):. 2019 [PMID: 30728336] (Bioassay, Human) Bioassay Human
R Hamnett, P Crosby, JE Chesham, MH Hastings Vasoactive intestinal peptide controls the suprachiasmatic circadian clock network via ERK1/2 and DUSP4 signalling Nat Commun, 2019;10(1):542. 2019 [PMID: 30710088] (Bioassay, Mouse) Bioassay Mouse
C Wu, ME Watts, LL Rubin MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis Cell Rep, 2019;26(5):1143-1156.e5. 2019 [PMID: 30699345] (Bioassay, Mouse) Bioassay Mouse
C Iwasawa, N Kuzumaki, Y Suda, R Kagawa, Y Oka, N Hattori, H Okano, M Narita Reduced expression of somatostatin in GABAergic interneurons derived from induced pluripotent stem cells of patients with parkin mutations Mol Brain, 2019;12(1):5. 2019 [PMID: 30658665] (Bioassay, Human) Bioassay Human
Z Melamed, J López-Erau, MW Baughn, O Zhang, K Drenner, Y Sun, F Freyermuth, MA McMahon, MS Beccari, JW Artates, T Ohkubo, M Rodriguez, N Lin, D Wu, CF Bennett, F Rigo, S Da Cruz, J Ravits, C Lagier-Tou, DW Cleveland Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration Nat. Neurosci., 2019;22(2):180-190. 2019 [PMID: 30643298] (Bioassay, Human) Bioassay Human
Show All 123 Publications.

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The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

BDNF Antibodies and Synaptic Research
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse...  Read full blog post.

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