Recombinant Human BDNF Protein


1 μg/lane of Recombinant Human BDNF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human BDNF (Catalog #248‑BD) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL.

Product Details

Reactivity Hu, Mu, Rt, Ca, EqSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human BDNF Protein Summary

Additional Information
Product is in the process of discontinuation.100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined.
Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL.
The specific activity of Recombinant Human BDNF is approximately 1.3 x 103 units/μg, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534).
Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) is coated at 1 μg/mL, Recombinant Human BDNF binds with an apparent Kd <1 nM.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived BDNF protein
Accession # P23560
100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined.
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13-14 kDa, reducing conditions
Read Publications using
248-BD in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BDNF Protein

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • neurotrophin


Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins (1, 2). All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor (3).

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

Customers Who Viewed This Item Also Viewed...

Species: Rt
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Ca
Applications: WB, IHC, ICC
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Mu, Rt
Applications: WB, Simple Western, IHC, Block
Species: Hu
Applications: WB, IP
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
Species: Hu, Mu, Rt, Ca, Eq
Applications: Bioactivity

Publications for BDNF (248-BD)(112)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Porcine, Primate - Callitrix jacchus (Common Marmoset).

We have publications tested in 6 applications: Bioassay, ELISA Standard, In Vivo, Surface Plasmon Resonance, WB Standard, cell culture.

Filter By Application
ELISA Standard
In Vivo
Surface Plasmon Resonance
WB Standard
cell culture
All Applications
Filter By Species
Primate - Callitrix jacchus (Common Marmoset)
All Species
Showing Publications 1 - 10 of 112. Show All 112 Publications.
Publications using 248-BD Applications Species
C Iwasawa, N Kuzumaki, Y Suda, R Kagawa, Y Oka, N Hattori, H Okano, M Narita Reduced expression of somatostatin in GABAergic interneurons derived from induced pluripotent stem cells of patients with parkin mutations Mol Brain, 2019;12(1):5. 2019 [PMID: 30658665] (Bioassay, Human) Bioassay Human
MB Huynh, MO Ouidja, S Chantepie, G Carpentier, A Maïza, G Zhang, J Vilares, R Raisman-Vo, D Papy-Garci Glycosaminoglycans from Alzheimer&#039;s disease hippocampus have altered capacities to bind and regulate growth factors activities and to bind tau PLoS ONE, 2019;14(1):e0209573. 2019 [PMID: 30608949] (Bioassay, Human) Bioassay Human
MA Wälti, J Steiner, F Meng, HS Chung, JM Louis, R Ghirlando, V Tugarinov, A Nath, GM Clore Probing the mechanism of inhibition of amyloid-?(1-42)-induced neurotoxicity by the chaperonin GroEL Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 30509980] (Bioassay, Human) Bioassay Human
K Ravina, DI Briggs, S Kislal, Z Warraich, T Nguyen, RK Lam, TI Zarembinsk, M Shamloo Intracerebral Delivery of Brain-Derived Neurotrophic Factor Using HyStem�-C Hydrogel Implants Improves Functional Recovery and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke Int J Mol Sci, 2018;19(12):. 2018 [PMID: 30486515] (In Vivo, Rat) In Vivo Rat
NV Vysokov, JP Silva, VG Lelianova, J Suckling, J Cassidy, JK Blackburn, N Yankova, MB Djamgoz, SV Kozlov, AG Tonevitsky, YA Ushkaryov Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones Elife, 2018;7(0):. 2018 [PMID: 30457553] (Bioassay, Rat) Bioassay Rat
G Giannotti, SM Barry, BM Siemsen, J Peters, JF McGinty Divergent Prelimbic Cortical Pathways Interact with BDNF to Regulate Cocaine Seeking J. Neurosci., 2018;0(0):. 2018 [PMID: 30185459] (In Vivo, Rat) In Vivo Rat
K Kirino, T Nakahata, T Taguchi, MK Saito Efficient derivation of sympathetic neurons from human pluripotent stem cells with a defined condition Sci Rep, 2018;8(1):12865. 2018 [PMID: 30150715] (Bioassay, Human) Bioassay Human
K Fujimori, M Ishikawa, A Otomo, N Atsuta, R Nakamura, T Akiyama, S Hadano, M Aoki, H Saya, G Sobue, H Okano Modeling sporadic ALS in iPSC-derived motor neurons identifies a potential therapeutic agent Nat. Med., 2018;0(0):. 2018 [PMID: 30127392] (Bioassay, Human) Bioassay Human
S Koyuncu, I Saez, HJ Lee, R Gutierrez-, W Pokrzywa, A Fatima, T Hoppe, D Vilchez The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington&#039;s disease patients Nat Commun, 2018;9(1):2886. 2018 [PMID: 30038412] (Bioassay, Human) Bioassay Human
N Tsuburaya, K Homma, T Higuchi, A Balia, H Yamakoshi, N Shibata, S Nakamura, H Nakagawa, SI Ikeda, N Umezawa, N Kato, S Yokoshima, M Shibuya, M Shimonishi, H Kojima, T Okabe, T Nagano, I Naguro, K Imamura, H Inoue, T Fujisawa, H Ichijo A small-molecule inhibitor of SOD1-Derlin-1 interaction ameliorates pathology in an ALS mouse model Nat Commun, 2018;9(1):2668. 2018 [PMID: 29991716] (Bioassay, Human) Bioassay Human
Show All 112 Publications.

Reviews for BDNF (248-BD) (0)

There are no reviews for BDNF (248-BD). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for BDNF (248-BD) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for BDNF (248-BD)

Discover related pathways, diseases and genes to BDNF (248-BD). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BDNF (248-BD)

Discover more about diseases related to BDNF (248-BD).

Pathways for BDNF (248-BD)

View related products by pathway.

PTMs for BDNF (248-BD)

Learn more about PTMs related to BDNF (248-BD).

Blogs on BDNF.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

BDNF Antibodies and Synaptic Research
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse...  Read full blog post.

Save Time & Money with High Quality Sandwich ELISA Kits
Enzyme-linked immunosorbent assay (ELISA) is a widely used technique for detecting concentration of proteins, using enzyme tagged antibodies with which react with dyes to produce a colorimetric or fluorescent signal. Sandwich ELISA takes this one step...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human BDNF Protein and receive a gift card or discount.


Gene Symbol BDNF