Recombinant Canine CD4 Protein, CF

• Reactivity: Ca
• Format: Carrier-Free

Recombinant Canine CD4 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived canine CD4 protein
  Val25-Lys401, with a C-terminal 6-His tag
• Accession #: P33705
• N-terminal Sequence: Val25
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 42-61 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Canine CD4 Protein, CF

• CD_antigen: CD4
• CD4 antigen (p55)
• CD4 antigen
• CD4 molecule
• CD4 receptor
• CD4
• CD4mut
• T-cell surface antigen T4/Leu-3
• T-cell surface glycoprotein CD4

Background

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes (1). CD4 is a standard phenotype marker for the identification of T cell populations (1). CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes (2, 3). CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV (4). Mature canine CD4 consists of a 377 aa extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the extracellular region, canine CD4 shares 57%, 46%, and 42% amino acid sequence identity with human, mouse, and rat CD4, respectively.

1. Vignali, D.A.A. (2010) J. Immunol. 184:5933.
2. Doyle, C. and J.L. Strominger (1987) Nature 330:256.
3. Huppa, J.B. et al. (2010) Nature 463:963.
4. Biswas, P. et al. (2003) Blood 101:4452.
5. Maddon, P.J. et al. (1985) Cell 42:93.

FAQs for CD4 (9646-CD). (Showing 1 - 1 of 1 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Uniprot:
  • P33705()