RBFOX3/NeuN Antibody (1B7) [Alexa Fluor® 700]

Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody (1B7) [Alexa Fluor® 700] [NBP1-92693AF700] - Expression of NeuN in mouse cortex. Image from verified customer review.

Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [Alexa Fluor® 700] [NBP1-92693AF700] - An intracellular stain was performed on U87-MG cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [Alexa Fluor (R) 700] [NBP1-92693AF700] - FACS analysis of mouse brain cells - Trypsin-EDTA digestion, 2% PFA fixation, primary used at 1:10 dilution/ 1 hour RT incubation (Image provided by Dr Xingrao Ke from Dept. of Pediatrics at Medical College of Wisconsin; see the product review for methodology details).

Flow (Intracellular): RBFOX3/NeuN Antibody (1B7) [Alexa Fluor 700] [NBP1-92693AF700] - An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 1B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

RBFOX3/NeuN Antibody (1B7) [Alexa Fluor® 700] Summary

• Immunogen: N-terminal 99 amino acids of human FOX3 as expressed in and purified from E. coli
• Localization: Nucleus. Cytoplasm.
• Marker: Neuronal Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBFOX3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Flow (Intracellular)
• Application Notes: This RBFOX3/Neun (1B7) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where bands can be seen at 46 and 48 kDa. Use in IHC-frozen sections was reported in the scientific literature (PMID: 23776455). FLOW application was reported by Dr Xingrao Ke from Dept. of Pediatrics at Medical College of Wisconsin - see the product review for methodology details.

Reactivity Notes

Human, bovine, porcine, mouse, rat and other mammals.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RBFOX3/NeuN Antibody (1B7) [Alexa Fluor® 700]

• FLJ56884
• FLJ58356
• Fox-1 homolog C
• FOX3
• FOX-3
• hexaribonucleotide binding protein 3
• HRNBP3
• NeuN
• neuronal nuclei
• RBFOX3
• RNA binding protein fox-1 homolog 3
• RNA binding protein, fox-1 homolog (C. elegans) 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBFOX3/NeuN Antibody (NBP1-92693AF700)(1)

Publication using NBP1-92693AF700

• Lutz PE, Tanti A, Gasecka A et al. Association of a History of Child Abuse With Impaired Myelination in the Anterior Cingulate Cortex: Convergent Epigenetic, Transcriptional, and Morphological Evidence Am J Psychiatry 2017 Jul 28 [PMID: 28750583] (Flow, Human)

Review for RBFOX3/NeuN Antibody (NBP1-92693AF700) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Subhash Gangar Flow Mouse 09/25/2014

Summary

• Application: Flow Cytometry
• Sample Tested: Mouse Brain Cells
• Species: Mouse
• Lot: 121712AF700
• Comments: Excitation Wavelength: 640nm
  
  Emission Filter: 719nm
  
  Events Acquired: 50000</p><p>Reviewed by Dr Xingrao Ke, Research Scientist II in the Dept. of Pediatrics in Medical College of Wisconsin.

Blocking

• Blocking Details: Mouse BD Fc Block (TM), 10 Minutes/RT

Primary Anitbody

• Dilution Ratio: 1:10 dilution in FACS buffer; Incubated 1 hour at RT

Details

• Detection Notes: Final suspension solution: 2% paraformaldehyde in PBS; Detected on LSRII
• Fixation Details: Cells fixed in 2% PFA- PBS at 4C ON; Permeablized for 20 minutes with BD Cytofix/CytopermTM Fixation/Permeabilization solution
• Wash Description: 3 Minutes single wash with FACS buffer (1x PBS with 2% fetal bovine serum and 0.1% Azide)

Comments

• Comments: Excitation Wavelength: 640nm
  
  Emission Filter: 719nm
  
  Events Acquired: 50000</p><p>Reviewed by Dr Xingrao Ke, Research Scientist II in the Dept. of Pediatrics in Medical College of Wisconsin.

Bioinformatics

• Gene Symbol: RBFOX3