RBFOX3/NeuN Antibody (1B7) - BSA Free

Formalin fixed rat brain stem neurons stained with to NeuN in green and counter stained with , our chicken polyclonal antibody to microtubule associated protein 2 in red. The nuclei of cells are revealed with DAPI in blue. The antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of other cell types. The MAP2 antibody binds to dendrites and overlaps with Fox3 staining in perikarya. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells.

Spinal cords from 60 days old nalve nontransgenic mice (endogenous) and transgenic mice overexpressing human WT- SOD1 were analyzed by immunohistochemistry for cellular distribution of activated p38 MAPK. Low magnification (10X objective) images of lumbar spinal cord double-labeled with a neuronal marker (NeuN, in green) and activated p38 MAPK (pP38, in red). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0065235), licensed under a CC-BY license.

Analysis of whole brain tissue lysates using mouse mAb to FOX3/NeuN NBP1-92693, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Note the strong twin bands corresponding to the two alternate transcripts of FOX3/NeuN protein with apparent SDS-PAGE molecular weights of 46 and 48kDa. As with other FOX3/NeuN antibodies, an additional band at ~70kDa is revealed in some lysates.

Using the Allophycocyanin direct conjugate An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693APC (blue) and a matched isotype control NB600-986APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Dissociated spinal cord nuclei immunostained with anti-NeuN antibody and Alexa 488 secondary. DAPI (Blue) to stain nuclei. This image was submitted by customer review.

An intracellular stain was performed on U87-MG cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Rat brain neural cultures stained with NBP1-92693 (red), a chicken polyclonal antibody to GFAP (NBP1-05198, green) and DAPI (blue). NBP1-92693 reveals strong nuclear and distal cytoplasmic staining for RBFOX/Neun and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. This RBFOX/Neun (1B7) antibody is therefore an excellent marker of neuronal cells.

IHC-Fr analysis of a 4% PFA fixed mouse spinal cord tissue section using RBFOX3/NeuN antibody (clone 1B7) at 1:500 dilution with overnight 4C incubation in a dilution buffer which contained BSA 1% and 0.2% TritonX100 in PBS. The signal was detected using Alexa Fluor 594 conjugated goat anti-mouse IgG (H+L) secondary antibody. The antibody generated a specific cytoplasmic-nuclear signal and the staining was more intense in the nuclei of the neurons. This image was submitted as a review via a verified end user of this product.

Analysis of PE conjugate of NBP1-92693. An intracellular stain was performed on SH-SY5Y cells with RBFOX3 (1B7) antibody NBP1-92693PE (blue) and a matched isotype control NB600-986PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Using the PerCP direct conjugate An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693PCP (blue) and a matched isotype control NB600-986PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-protein.

An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image from the Alexa Fluor 700 version of this antibody.

An intracellular stain was performed on A549 cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - NF kappa B localizes to both neuronal & glial nuclei in superior colliculus. Representative confocal images of NF kappa B in the superior colliculus (SC) of vehicle- & HE3286-treated rats with all nuclei indicated (DAPI). Sections were also labeled for astrocytes (A, GFAP), microglia (B, Iba1), or neurons using antibodies against either NeuN (C) or phosphorylated neurofilament heavy (pNFH; D). Representative nuclei from each cell class demonstrated NF kappa B localization (arrows). Insets (solid white, B) show region contained within dashed box with red channel removed to better visualize nuclear localization of NF kappa B. Scale: 10 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnins.2017.00045/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 1B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RBFOX3/NeuN Antibody (1B7) - BSA Free Summary

• Immunogen: N-terminal 99 amino acids of human FOX3 as expressed in and purified from E. coli
• Localization: Nucleus. Cytoplasm.
• Marker: Neuronal Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBFOX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 1:10
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID: 30654114/ 23776455)
  • Immunohistochemistry-Paraffin
  • Western Blot 1:5000-1:10000
• Application Notes: This RBFOX3/Neun (1B7) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where bands can be seen at 46 and 48 kDa. Flow Cytometry was reported in verified customer review using Alexa Fluor 700 conjugated form of this antibody, NBP1-92693AF700. This antibody is CyTOF ready.
• Theoretical MW: 33.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBFOX3/NeuN Antibody (1B7) - BSA Free

• FLJ56884
• FLJ58356
• Fox-1 homolog C
• FOX3
• FOX-3
• hexaribonucleotide binding protein 3
• HRNBP3
• neuN antigen
• NeuN flow cytometry
• NEUN
• neuronal nuclei antigen
• neuronal nuclei
• RBFOX3
• RNA binding protein fox-1 homolog 3
• RNA binding protein, fox-1 homolog (C. elegans) 3
• RNA binding protein, fox-1 homolog 3

Background

Rbfox3 (also known as Fox-3, Fox1 homolog C, Hrnbp3, and D11Bwg0517e) is one of three mammalian members of the RNA-binding protein Rbfox gene family, all of which are involved in regulating alternative RNA splicing. Originally identified as an epitope, NeuN is located within the N-terminal region (6-15 amino acids) of Rbfox3. Rbfox family members are highly conserved, having a single RNA recognition motif (RRM)-type RNA binding domain (RBD) near the center of the protein sequence. The RBD amino acid (aa) sequence is identical between members, Rbfox1 and Rbfox2, with 4 differences within the 77 aa domain of Rbfox3. The fox3 gene is comprised of 15 exons in humans, 11 exons in rat, with 3 variants in mouse containing 14 exons and another 3 variants containing 15 exons. The Rbfox3 protein is highly conserved across human, mouse, and rat with 98.9% sequence identity between mouse (isoform I) and rat and 83.9% sequence identity between mouse (isoform I) and human. The expression of Rbfox3/NeuN is restricted to the nervous system and has widely been used in stroke research. It is recognized as a marker of mature neuronal cell types in the spinal cord, cerebral cortex, hippocampus, dorsal thalamus, caudate/putamen, and cerebellum. NeuN has been shown to bind DNA and is predominantly nuclear. Differences in immunoreactivity have been reported between Rbfox3/NeuN subtypes in which the 46kDa is mainly found in the nucleus whereas the 48kDa form is primarily distributed in the cytoplasm (1,2).

Rbfox proteins participate in regulation of alternative splicing between family members as well as in autoregulation. While the breadth of brain and muscle specific targets for alternative splicing is well established for Rbfox proteins, those specific to Rbfox3/NeuN along with its alternative splicing mechanism are less understood. Rbfox3 has been shown to regulate neuronal differentiation by alternative splicing of Numb pre-mRNA and has a role in adult neurogenesis. Dysfunctional Rbfox3/NeuN has been associated with various neurological disorders such as neurodevelopmental delay, autism spectrum disorder, Benign rolandic epilepsy (BRE), and cognitive impairments (1,2).

References

1. Duan W, Zhang YP, Hou Z, Huang C, Zhu H, Zhang CQ, Yin Q. (2016) Novel Insights into NeuN: from Neuronal Marker to Splicing Regulator. Mol Neurobiol. 53(3):1637-1647. PMID: 25680637.

2. Su CH, D D, Tarn WY. (2018) Alternative Splicing in Neurogenesis and Brain Development. Front Mol Biosci. 5:12. PMID: 29484299

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBFOX3/NeuN Antibody (NBP1-92693)(67)

Publications using NBP1-92693

• Litif CG, Flom LT, Sandum KL et al. Sex-Dependent Genetic Expression Signatures within Cocaine- and Sucrose-Seeking Ensembles in Mice bioRxiv : the preprint server for biology 2023-11-05 [PMID: 37961222] (Mouse)
• Wu DM, Liu JP, Liu J et al. Immune pathway activation in neurons triggers neural damage after stroke Cell reports 2023-11-01 [PMID: 37917581] (Immunohistochemistry, Mouse)
• Ernst AS. Impact of the receptor tyrosine kinase EphB2 on cerebral ischemia in mice. Thesis. 2018-01-01 (ICC/IF)
• Philtjens S, Turnbull MT, Thedy BP et al. Chemogenetic activation of astrocytes in the hippocampus and cortex changes the transcriptome of microglia and other cell types. bioRxiv 2020-01-01 (IF/IHC)
• Velmurugan GV, Vekaria HJ, Patel SP et Al. Astrocytic mitochondrial transfer to brain endothelial cells and pericytes in vivo increases with aging J Cereb Blood Flow Metab 2024-12-12 [PMID: 39668588]
• Yi Y, Che W, Xu P, Mao C et Al. Conversion of glioma cells into neuron-like cells by small molecules iScience 2024-11-01 [PMID: 39483145]
• Van Duyne R, Irollo E, Lin A, Johnson JA, Guillem AM, O'Brien EV, Merja L, Nash B, Jackson JG, Sarkar A, Klase ZA, Meucci O. Adult Human Brain Tissue Cultures to Study NeuroHIV. Cells 2024-07-12 [PMID: 38994979]
• Lancaster MS, Kim B, Doud EH, Tate MD et Al. Loss of succinyl-CoA synthetase in mouse forebrain results in hypersuccinylation with perturbed neuronal transcription and metabolism Cell Rep 2023-10-11 [PMID: 37819759]
• Neogi R Involvement of the Sigma-1 Receptor in Methamphetamine-Mediated Changes to Astrocyte Structure and Function Thesis 2020-01-01 (IF/IHC)
• Tanabe M, Kunisawa K, Saito I et Al. Adolescent social isolation decreases colonic goblet cells and impairs spatial cognition through the reduction of cystine Mol Psychiatry 2024-11-29 [PMID: 39613916]
• Weaver FE, White E, Peek AM et Al. 4-Phenylbutyric acid mitigates ER stress-induced neurodegeneration in the spinal cords of a GM2 gangliosidosis mouse model Hum Mol Genet 2024-11-12 [PMID: 39530163]
• Xu C, Wang Y, Ni C et Al. Histone modifications and Sp1 promote GPR160 expression in bone cancer pain within rodent models EMBO Rep 2024-10-24 [PMID: 39448865]
• Paramasivam, P;Choi, SW;Poddar, R;Paul, S; Impairment of neuronal tyrosine phosphatase STEP worsens post-ischemic inflammation and brain injury under hypertensive condition Journal of neuroinflammation 2024-10-22 [PMID: 39438980]
• Chen Y, Wu XL, Hu HB et al. Neuronal MeCP2 in the dentate gyrus regulates mossy fiber sprouting of mice with temporal lobe epilepsy Neurobiology of disease 2023-11-01 [PMID: 37931884] (IHC, Mouse)
  
  Details:
  Dilution 1:2000
• Cicardi ME, Hallgren JH, Mawrie D et al. C9orf72 poly(PR) mediated neurodegeneration is associated with nucleolar stress iScience 2023-09-15 [PMID: 37664610] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• Milstead R, Link C, Xu Z, Hoeffer C Molecular Signaling and Neural Cell Type Expression of Complex Brain Disorders Thesis Jul 13 1905 (IHC-Fr, Human, Mouse)
• Gulen MF, Samson N, Keller A et al. cGAS-STING drives ageing-related inflammation and neurodegeneration Nature 2023-08-01 [PMID: 37532932]
  
  Details:
  scRNA-seq
• Ingiosi A, Hayworth C, Frank M Activation of basal forebrain astrocytes induces wakefulness without compensatory changes in sleep drive bioRxiv 2023-01-10 [PMID: 37487739] (IHC, Mouse)
• Zhang H, Guo Y, Yang Y et al. MAP4Ks inhibition promotes retinal neuron regeneration from M�ller glia in adult mice npj Regenerative Medicine 2023-07-13 [PMID: 37443319] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• Li H, Heath J, Trippett J Et al. Engineering Viral Vectors for Acoustically Targeted Gene Delivery biorxiv.org 2021-07-27 (Mouse)
• Howell JA, Gaouette N, Lopez M, Burke SP et Al. Elastin-like polypeptide delivery of anti-inflammatory peptides to the brain following ischemic stroke FASEB J 2023-07-04 [PMID: 37402128]
• Moffet JJD, Moore Z, Oliver SJ et al. Flow Cytometry Identification of Cell Compartments in the Murine Brain Methods in molecular biology (Clifton, N.J.) 2023-06-24 [PMID: 37355546]
• Pandey M, Zhang JH, Adikaram PR et al. Specific regulation of mechanical nociception by G?5 involves GABA-B receptors JCI insight 2023-05-23 [PMID: 37219953] (IHC-Fr, Mouse)
  
  Details:
  Dilutions: 1:200
• Bryant A, Li Z, Jayakumar R et al. Endothelial Cells Are Heterogeneous in Different Brain Regions and Are Dramatically Altered in Alzheimer's Disease The Journal of Neuroscience 2023-06-14 [PMID: 37208174]
• Festa BP, Siddiqi FH, Jimenez-Sanchez M et al. Microglial-to-neuronal CCR5 signaling regulates autophagy in neurodegeneration Neuron 2023-04-18 [PMID: 37105172] (ChIP, Mouse)
• Howell JA, Gaouette N, Lopez M et al. Elastin-like polypeptide delivery of anti-inflammatory peptides to the brain following ischemic stroke bioRxiv : the preprint server for biology 2023-03-15 [PMID: 36993686] (IHC-Fr, Rat)
  
  Details:
  Dilutions: 1:1000
• Shekhtmeyster P, Carey EM, Duarte D et al. Multiplex translaminar imaging in the spinal cord of behaving mice Nature communications 2023-03-21 [PMID: 36944637] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC-Fr 1:100
• Ou M, Chen Y, Liu J et al. Spinal astrocytic MeCP2 regulates Kir4.1 for the maintenance of chronic hyperalgesia in neuropathic pain Progress in neurobiology 2023-05-01 [PMID: 36931588] (FLOW, Mouse)
• Dawes P, Smullen M, Fernandez-Fontaine A et al. FlowSeq: A multiplexed quantitative approach to identify genes affecting cell type enrichment using mosaic CRISPR-Cas9 edited cerebral organoids Research Square 2022-08-01 [PMID: 36877372] (FLOW)
• Bryant A, Li Z, Jayakumar R et al. Endothelial Cells are Heterogeneous in Different Brain Regions and are Dramatically Altered in Alzheimer's Disease bioRxiv : the preprint server for biology 2023-02-16 [PMID: 36824974] (ICC/IF, Human)
• Cicardi ME, Hallgren JH, Mawrie D et al. C9orf72 poly(PR) mediated neurodegeneration is associated with nucleolar stress bioRxiv 2023-02-16 [PMID: 36824930] (ICC/IF)
• Tang AT, Buchholz DW, Szigety KM et al. Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection PLoS biology 2023-02-01 [PMID: 36745682] (IHC, Mouse)
• Milstead RA, Link CD, Xu Z, Hoeffer CA TDP-43 knockdown in mouse model of ALS leads to dsRNA deposition, gliosis, and neurodegeneration in the spinal cord Cerebral cortex (New York, N.Y. : 1991) 2022-11-28 [PMID: 36443249]
• Milstead R, Link C, Xu Z, Hoeffer C Molecular Signaling and Neural Cell Type Expression of Complex Brain Disorders Thesis Jul 13 1905 (IHC-Fr, Human, Mouse)
• Delic V, Karp JH, Guzman M et al. Repetitive mild TBI causes pTau aggregation in nigra without altering preexisting fibril induced Parkinson's-like pathology burden Acta neuropathologica communications 2022-11-26 [PMID: 36435806] (WB, Rat)
  
  Details:
  Additional file 4; 0.5 ug/ml
• Herring CA, Simmons RK, Freytag S Et al. Human prefrontal cortex gene regulatory dynamics from gestation to adulthood at single-cell resolution Cell 2022-11-01 [PMID: 36318921] (IHC-Fr, Human)
  
  Details:
  Citation using the Allophycocyanin version of this antibody.
• Xu C, Zhao B, Xu L et al. CXCR1 participates in bone cancer pain induced by Walker 256 breast cancer cells in female rats Molecular pain 2022-04-01 [PMID: 36227008] (IF/IHC, Rat)
• Ben-Simon Y, Kaefer K, Velicky P et al. A direct excitatory projection from entorhinal layer 6b neurons to the hippocampus contributes to spatial coding and memory Nature communications 2022-08-16 [PMID: 35974109] (IF/IHC, Mouse)
  
  Details:
  Supplementary Figure 5: Dilutions: 1:500
• Kim S, Sohn S, Choe ES. Phosphorylation of GluA1-Ser831 by CaMKII Activation in the Caudate and Putamen Is Required for Behavioral Sensitization After Challenge Nicotine in Rats International Journal of Neuropsychopharmacology 2022-08-16 [PMID: 35678163]
• Varn FS, Johnson KC, Martinek J Et al. Glioma progression is shaped by genetic evolution and microenvironment interactions Cell 2022-06-01 [PMID: 35649412] (IHC-P, Human)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Wong H, Buck JM, Borski C et al. RCAN1 knockout and overexpression recapitulate an ensemble of rest-activity and circadian disruptions characteristic of Down syndrome, Alzheimer's disease, and normative aging Journal of Neurodevelopmental Disorders 2022-12-01 [PMID: 35610565] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• Chen W, Li Y, Steinhoff M et al. The PLAUR signaling promotes chronic pruritus The FASEB Journal 2022-06-01 [PMID: 35596683] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• Peinado MA, Ovelleiro D, Del Moral ML et al. Biological Implications of a Stroke Therapy Based in Neuroglobin Hyaluronate Nanoparticles. Neuroprotective Role and Molecular Bases International journal of molecular sciences 2021-12-27 [PMID: 35008673] (IF/IHC, Rat)
• Ma KG, Hu HB, Zhou JS Et al. Neuronal Glypican4 promotes mossy fiber sprouting through the mTOR pathway after pilocarpine-induced status epilepticus in mice Experimental neurology 2021-11-05 [PMID: 34748756] (IF/IHC, Mouse)
• Qian J, Tu H, Zhang D Et al. Therapeutic effects of masitinib on abnormal mechanoreception in a mouse model of tourniquet-induced extremity ischemia-reperfusion European journal of pharmacology 2021-11-15 [PMID: 34619116] (IF/IHC, Mouse)
• Levenga J, Wong H, Milstead R et al. Immunohistological Examination of AKT Isoforms in the Brain: Cell-Type Specificity that May Underlie AKT’s Role in Complex Brain Disorders and Neurological Disease Cereb Cortex Commun 2021-07-23 [PMID: 34296180]
• Peterson S, Maheras A, Chavira J et al. Sex Differences in Discrimination Behavior and Orbitofrontal Engagement During Context-Gated Reward Prediction bioRxiv 2023-08-30 (IHC-FrFl, Rat)
• Gerrits E, Giannini LAA, Brouwer N Et al. Neurovascular dysfunction in GRN-associated frontotemporal dementia identified by single-nucleus RNA sequencing of human cerebral cortex Nat Neurosci 2022-07-25 [PMID: 35879464] (FLOW, Human)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Jeon H, Kim M, Park W et al. Upregulation of AQP4 Improves Blood-Brain Barrier Integrity and Perihematomal Edema Following Intracerebral Hemorrhage Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2021-09-20 [PMID: 34545550] (ICC/IF, Mouse)
• Gu L, Kwong J, Caprioli J, Piri N Loss of Rbfox1 Does Not Affect Survival of Retinal Ganglion Cells Injured by Optic Nerve Crush Frontiers in Neuroscience 2021-05-24 [PMID: 34108862] (IF/IHC, Mouse)
• Anyaegbu CC, Mao Y, McGonigle T et al. Simultaneous flow cytometric characterization of multiple cell types and metabolic states in the rat brain after repeated mild traumatic brain injury Journal of neuroscience methods 2021-05-15 [PMID: 34004202] (IF/IHC, Rat)
• Wong H, Levenga J, LaPlante L, et al. Isoform-specific roles for AKT in affective behavior, spatial memory, and extinction related to psychiatric disorders eLife 2020-12-16 [PMID: 33325370] (IF/IHC)
• Semmler S, GagnE M, Garg P et al. TNF receptor associated factor 6 interacts with ALS-linked misfolded superoxide dismutase 1 and promotes aggregation J. Biol. Chem. 2020-02-06 [PMID: 32029478]
• Yin, C, Liu, B Et al. Eucalyptol alleviates inflammation and pain responses in a mouse model of gout arthritis. Br J Pharmacol 2020-05-01 [PMID: 31883118]
• Tejeda GS, Esteban-Ortega GM, San Antonio E et Al. Prevention of excitotoxicity-induced processing of BDNF receptor TrkB-FL leads to stroke neuroprotection EMBO Mol Med 2019-06-03 [PMID: 31273936]
• Yun H, Park ES, Choi S et al TDAG51 is a crucial regulator of maternal care and depressive-like behavior after parturition. PLoS Genet. 2019-06-28 [PMID: 31251738] (IF/IHC, Mouse)
  
  Details:
  Citation using the Alexa Fluor 405 version of this antibody.
• Prevention of excitotoxicity-induced processing of BDNF receptor TrkB-FL leads to stroke neuroprotection. Tejeda G, Esteban-Ortega G, San Antonio E, et al. EMBO Mol Med 2019-06-03 [PMID: 31160342] (IF, Mouse, Rat)
• Chen X, Lin S, Gu L et al. Inhibition of miR-497 improves functional outcome after ischemic stroke by enhancing neuronal autophagy in young and aged rats. Neurochem. Int. 2019-01-15 [PMID: 30654114] (IHC-P, Rat)
• Robinson M, Fraser I, McKee E et al. Transdifferentiating Astrocytes Into Neurons Using ASCL1 Functionalized With a Novel Intracellular Protein Delivery Technology Front Bioeng Biotechnol 2018-11-21 [PMID: 30525033] (ICC/IF, Human)
• Ding Z, Cao J, Shen Y et al. Resveratrol Promotes Nerve Regeneration via Activation of p300 Acetyltransferase-Mediated VEGF Signaling in a Rat Model of Sciatic Nerve Crush Injury Front Neurosci. [PMID: 29875625] (IF/IHC, Rat)
• Wang P, Jiang L, Zhou N et al. Resveratrol ameliorates autophagic flux to promote functional recovery in rats after spinal cord injury. Oncotarget 2018-02-02 [PMID: 29492205] (Rat)
• Gatto RG, Li W, Magin RL. Diffusion Tensor Imaging Identifies Presymptomatic Axonal Degeneration in the Spinal Cord of ALS Mice Brain Res. 2017-11-23 [PMID: 29175489] (Mouse)
• Levenga J, Wong H, Milstead RA et al. AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity. Elife 2017-11-27 [PMID: 29173281] (IHC-Fr, Mouse)
• Lutz PE, Tanti A, Gasecka A et al. Association of a History of Child Abuse With Impaired Myelination in the Anterior Cingulate Cortex: Convergent Epigenetic, Transcriptional, and Morphological Evidence Am J Psychiatry 2017 Jul 28 [PMID: 28750583] (Flow, Human) Am J Psychiatry. 2017-07-28 [PMID: 28750583] (FLOW, Human)
  
  Details:
  Citation using the Alexa Fluor 700 form of this antibody.
• Cohen S, Ke X, Liu Q et al. Adverse early life environment increases hippocampal microglia abundance in conjunction with decreased neural stem cells in juvenile mice Int. Int. J. Dev. Neurosci. 2016-09-22 [PMID: 27666383] (FLOW, Mouse)
  
  Details:
  This citation used the Allophycocyanin version of this antibody.
• Morfini GA, Bosco DA, Brown H et al. Inhibition of Fast Axonal Transport by Pathogenic SOD1 Involves Activation of p38 MAP Kinase. PLoS One 2013-06-12 [PMID: 23776455] (IHC-Fr, ICC/IF, Mouse)

Reviews for RBFOX3/NeuN Antibody (NBP1-92693) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer Immunostaining of dissociated spinal nuclei Mouse 01/26/2018

Summary

• Application: Other
• Sample Tested: Dissociated spinal cord nuclei
• Species: Mouse
• Lot: 082317

reviewed by: Verified Customer IHC-Fr Mouse 05/11/2016

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse spinal cord
• Species: Mouse
• Lot: 42514

Bioinformatics

• Gene Symbol: RBFOX3
• Entrez:
  • 146713(Human)
• Uniprot:
  • A6NFN3(Human)