Rad17 Antibody

Western Blot: Rad17 Antibody [NBP2-27106] - Western blot analysis of Rad17 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-27106 at 3 ug/ml.

• Reactivity: Hu, Mu, Pm, Bv, Pm, Eq, Pm-Or, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Rad17 Antibody Summary

• Description: Novus Biologicals Rabbit Rad17 Antibody (NBP2-27106) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was raised against RAD17
• Predicted Species: Rhesus Macaque (100%), Orangutan (100%), Bovine (92%), Chimpanzee (100%), Equine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 3-5ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 85% homologous in Canine and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rad17 Antibody

• CCYC
• cell cycle checkpoint protein (RAD17)
• cell cycle checkpoint protein RAD17
• FLJ41520
• HRAD17
• R24L
• RAD1 (S. pombe) homolog
• RAD1 homolog
• RAD17 homolog (S. pombe)
• Rad17
• Rad17-like protein
• RAD17SP
• RAD24
• RF-C activator 1 homolog
• RF-C/activator 1 homolog

Background

Rad17, also known as 'cell cycle checkpoint protein RAD17,' is a nuclear protein belonging to the Rad17/RAD24 family and necessary for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 is known to have a weak ATPase activity and is required for binding to chromatin and also participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEk1 activation. It may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination. Rad17 is a part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5. Four isoforms have been reported by X-ray irradiation. Overexpression of this protein is seen in various cancer cell lines and in colon carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD17
• Uniprot:
  • O75943()