![Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0045.jpg "Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit Rad17 [p Ser645] Antibody - BSA Free (NB100-273) is a polyclonal antibody validated for use in IHC and WB. Anti-Rad17 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | A synthetic peptide, which represented a portion of human Rad17 (LocusLink ID 5884) around serine 645. |
| Modification | p Ser645 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAD17 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
| Publication using NB100-273 | Applications | Species |
|---|---|---|
| Ge XQ, Jackson DA, Blow JJ et al. Dormant origins licensed by excess Mcm2-7 are required for human cells to survive replicative stress. Genes Dev 2007-12-01 [PMID: 18079179] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Rad17 Antibody (NB100-273)Find related products by research area.
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![Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0049.jpg "Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Whole cell lysate (50 ug) from HeLa cells before (-) or after (+) treatment with 10 Gy ionizing radiation. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0002.jpg "Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Whole cell lysate (50 ug) from HeLa cells before (-) or after (+) treatment with 10 Gy ionizing radiation. Antibody used at 1 ug/ml.")
![Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0048.jpg "Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Western Blot PP2A alpha [p Tyr307] Antibody [Unconjugated]](https://images.novusbio.com/images2/PP2A_AF3989_Western_Blot_5373.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")