PXR/NR1I2 Antibody

Western Blot: PXR/NR1I2 Antibody [NBP1-28799] - (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PXR/NR1I2 Antibody [NBP1-28799] - (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PXR/NR1I2 Antibody Summary

• Immunogen: Peptide with sequence EQFAITLKSYIECNR corresponding to internal region according to NP_003880.3, NP_071285.1, NP_148934.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NR1I2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1-3 ug/ml
• Application Notes: Western blot: Approx. 48kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8kDa according to NP_003880.3). An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Small Intestine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PXR/NR1I2 Antibody

• BXR
• NR1I2
• nuclear receptor subfamily 1 group I member 2
• nuclear receptor subfamily 1, group I, member 2
• ONR1
• Orphan nuclear receptor PAR1
• Orphan nuclear receptor PXR
• PAR
• PAR1
• PAR2
• Pregnane X receptor
• PRR
• PXR
• PXRPARq
• SAR
• Steroid and xenobiotic receptor
• SXR
• SXRBXR

Background

This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PXR/NR1I2 Antibody (NBP1-28799)(1)

Publication using NBP1-28799

• Thakur JK, Arthanari H, Yang F et al. A nuclear receptor-like pathway regulating multidrug resistance in fungi. Nature 2008-04-01 [PMID: 18385733]

Bioinformatics

• Gene Symbol: NR1I2
• Entrez:
  • 8856(Human)
• Uniprot:
  • NP_003880.3(Human)