PAR1/Thrombin Receptor Antibody (N2-11)

Images

 
Orthogonal Strategies: Immunohistochemistry: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - PAR1 is expressed by neural stem cells in the sub-ventricular zone (SVZ) of the adult mouse brain. ...read more
Western Blot: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Analysis of Thrombin Receptor expression in human testis using NBP1-71770.
Orthogonal Strategies: Immunohistochemistry: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - PAR1 is expressed by neural stem cells in the sub-ventricular zone (SVZ) of the adult mouse brain. ...read more
Immunocytochemistry/ Immunofluorescence: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Thrombin Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI ...read more
Western Blot: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Analysis of PAR1 in whole cell lysate of Neuro2A cell line using 4ug/ml concentration of PAR1 antibody (clone N2-11). The antibody detected a strong ...read more
Immunohistochemistry: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Analysis of Thrombin Receptor on mouse skin using NBP1-71770.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
N2-11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

PAR1/Thrombin Receptor Antibody (N2-11) Summary

Immunogen
The oligopeptide CNATLDPRSFLL from human Thrombin Receptor. [Swiss-Prot# P25116]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
F2R
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31885791).
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-71770 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PAR1/Thrombin Receptor Antibody (N2-11)

  • Cf2r
  • CF2RHTR
  • coagulation factor II (thrombin) receptor
  • Coagulation factor II receptor
  • F2R
  • HTR
  • PAR1
  • PAR1PAR-1protease-activated receptor 1
  • Protease-Activated Receptor 1
  • Thrombin receptor
  • ThrR
  • TRGPC
  • TRproteinase-activated receptor 1

Background

Thrombin, the main effector protease of coagulation cascade, is a most potent platelet activator and its actions on platelets are mediated by Thrombin Receptors (also called platelet protease-activated receptors, PARs), which are GPCR members of 7-transmembrane domain receptor superfamily. PARs subtypes includes: PAR-1, PAR-2, PAR-3, and PAR-4, and in addition to platelets, they are also located in smooth muscle cells, endothelial cells, and fibroblasts. PAR1 or thrombin receptor (F2R) is a prototypical GPCR activated by thrombin's lowest concentration compared to other PARs and after cleavage of the extreme N-terminal peptide, the exposed tethered ligand of PAR1 activates itself for participation in diverse physiological processes including coagulation, inflammation, and vascular homeostasis. PAR1 knockout in mouse leads to death of pups in midgestation due to impaired vascular development as well as severe bleeding, whereas endothelial cell-specific reintroduction of PAR1 can rescue the phenotype. PAR1 has emerged as a target for anti-platelet therapies which are useful for ACS (acute coronary syndromes), stable coronary disease, ischemic atherothrombotic stroke, and peripheral arterial disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PAR1/Thrombin Receptor Antibody (NBP1-71770)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-71770 Applications Species
Chen R, Cao X, Luo W et al. Dabigatran Suppresses PAR-1/SphK/S1P Activation of Astrocytes in Experimental Autoimmune Encephalomyelitis Model Front Mol Neurosci Jun 30 2020 [PMID: 32694981]
Nishimura F, Mogami H, Moriuchi K et al. Mechanisms of thrombin-Induced myometrial contractions: Potential targets of progesterone PLoS ONE May 4 2020 [PMID: 32365105] (IHC-P, Human) IHC-P Human
Kim MJ, Park KH, Lee JY et al. Weisheng-Tang Ameliorates Acute Ischemic Brain Damage in Mice by Maintaining Blood-Brain Barrier Integrity Oxid Med Cell Longev Dec 3 2019 [PMID: 31885791] (IHC-Fr, Mouse) IHC-Fr Mouse
Komori M, Ago T, Wakisaka Y, et al. Early initiation of a factor Xa inhibitor can attenuate tissue repair and neurorestoration after middle cerebral artery occlusion Brain Res. May 16 2019 [PMID: 31103522] (IHC, Mouse) IHC Mouse
Choi CI, Yoon H, Drucker KL et al. The Thrombin Receptor Restricts Subventricular Zone Neural Stem Cell Expansion and Differentiation Sci Rep Jun 19 2018 [PMID: 29921916] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Kim HN, Kim YR, Ahn SM et al. Protease activated receptor-1 antagonist ameliorates the clinical symptoms of experimental autoimmune encephalomyelitis via inhibiting breakdown of blood brain barrier. J. Neurochem. 2015 Aug 18 [PMID: 26285165] (WB, Mouse) WB Mouse
Gonda K, Watanabe TM, Ohuchi N, Higuchi H. In vivo nano-imaging of membrane dynamics in metastatic tumor cells using quantum dots. J Biol Chem;285(4):2750-7. 2010 Jan 22. [PMID: 19917603] (ICC/IF, Mouse) ICC/IF Mouse

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PAR1/Thrombin Receptor Antibody (NBP1-71770). (Showing 1 - 1 of 1 FAQs).

  1. Would you be able to confirm whether or not the peptide immunogen used to generate NBP1-71770 - PAR1/Thrombin Receptor Antibody (N2-11) is available for purchase?
    • Unfortunately for NBP1-71770, this product is a licensed monoclonal and we do not have authority to provide the immunizing peptide for purchase.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-71770AF350
Alexa Fluor 405 NBP1-71770AF405
Alexa Fluor 488 NBP1-71770AF488
Alexa Fluor 532 NBP1-71770AF532
Alexa Fluor 594 NBP1-71770AF594
Alexa Fluor 647 NBP1-71770AF647
Alexa Fluor 700 NBP1-71770AF700
Alexa Fluor 750 NBP1-71770AF750
Biotin NBP1-71770B
DyLight 350 NBP1-71770UV
DyLight 405 NBP1-71770V
DyLight 488 NBP1-71770G
DyLight 550 NBP1-71770R
DyLight 594 NBP1-71770DL594
DyLight 650 NBP1-71770C
DyLight 680 NBP1-71770FR
DyLight 755 NBP1-71770IR
FITC NBP1-71770F
HRP NBP1-71770H
Janelia Fluor 549 NBP1-71770JF549
Janelia Fluor 646 NBP1-71770JF646

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Bioinformatics

Gene Symbol F2R
Entrez
Uniprot