MARK2 [p Thr595] Antibody

Images

 
Western Blot: MARK2 [p Thr595] Antibody [NBP1-78028] - Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 ug per lane. Used at a dilution of ...read more
Western Blot: MARK2 [p Thr595] Antibody [NBP1-78028] - Lane 1: 293T cells untransfected. Lane 2: 293T cells transfected with MARK2 and PKC-zeta. Lane 3: 293T cells transfected with MARK2. Load: 35 ug per lane. Used at a ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MARK2 [p Thr595] Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595.
Modification
p Thr595
Specificity
This affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MARK2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:1000
  • ELISA 1:1000-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Expect a band at approximately 83 kDa corresponding - MARK2 by western blotting in the appropriate cell lysate or extract.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-78028 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MARK2 [p Thr595] Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • ELKL motif kinase 1
  • ELKL motif kinase
  • EMK-1
  • EMK1PAR-1
  • MAP/microtubule affinity-regulating kinase 2MGC99619
  • PAR1 homolog
  • Par1b
  • Ser/Thr protein kinase PAR-1B
  • serine/threonine protein kinase EMK
  • serine/threonine-protein kinase MARK2

Background

This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be co-expressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for MARK2 Antibody (NBP1-78028) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-78028:
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Images Ratings Applications Species Date Details
Western Blot MARK2 NBP1-78028
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reviewed by:
Anonymous
WB Rat 10/29/2015
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Summary

ApplicationWestern Blot
Sample TestedRat Sertoli cell lysate
SpeciesRat
Lot19270

Blocking

Blocking Details5% (w/v) non-fat milk in TBST for 1h @RT

Primary Anitbody

Dilution Ratio1:500, O/N @4C

Details

Detection NotesECL, ImageQuant™ LAS 4000 mini, Exposure time: 2 min

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MARK2 Antibody (NBP1-78028) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for MARK2 Antibody (NBP1-78028)

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Anonymous
10/29/2015
Application: WB
Species: Rat

Bioinformatics

Gene Symbol MARK2