MARK2 [p Thr595] Antibody

Western Blot: MARK2 [p Thr595] Antibody [NBP1-78028] - Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 ug per lane. Used at a dilution of 1:500 for overnight at 4C.

Western Blot: MARK2 [p Thr595] Antibody [NBP1-78028] - Lane 1: 293T cells untransfected. Lane 2: 293T cells transfected with MARK2 and PKC-zeta. Lane 3: 293T cells transfected with MARK2. Load: 35 ug per lane. Used at a dilution of 1:1000 for overnight at 4C.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

MARK2 [p Thr595] Antibody Summary

• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to Thr595.
• Modification: p Thr595
• Specificity: This affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MARK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:250-1:1000
  • ELISA 1:1000-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blot. Expect a band at approximately 83 kDa corresponding - MARK2 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Cross reactivity with MARK2 from other sources has not been tested.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MARK2 [p Thr595] Antibody

• EC 2.7.11
• EC 2.7.11.1
• ELKL motif kinase 1
• ELKL motif kinase
• EMK-1
• EMK1PAR-1
• MAP/microtubule affinity-regulating kinase 2MGC99619
• PAR1 homolog
• Par1b
• Ser/Thr protein kinase PAR-1B
• serine/threonine protein kinase EMK
• serine/threonine-protein kinase MARK2

Background

This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be co-expressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MARK2 Antibody (NBP1-78028) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Anonymous WB Rat 10/29/2015

Summary

• Application: Western Blot
• Sample Tested: Rat Sertoli cell lysate
• Species: Rat
• Lot: 19270

Blocking

• Blocking Details: 5% (w/v) non-fat milk in TBST for 1h @RT

Primary Anitbody

• Dilution Ratio: 1:500, O/N @4C

Details

• Detection Notes: ECL, ImageQuant™ LAS 4000 mini, Exposure time: 2 min

Bioinformatics

• Gene Symbol: MARK2