Profilin 2 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP3-00301] - Immunochemical staining of human Profilin 2 in human skeletal muscle with NBP3-00301 (1:10000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP3-00301] - Immunochemical staining of human Profilin 2 in human kidney with NBP3-00301 (1:10000, formalin-fixed paraffin embedded sections).

Western Blot: Profilin 2 Antibody [NBP3-00301] - NBP3-00301 at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:15 kDa Observed band size:13 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Profilin 2 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human Profilin 2 (Accession#: AAH18049.1; Met1-Phe140)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PFN2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunohistochemistry-Paraffin 1:2500-1:10000
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Profilin 2 Antibody - Azide and BSA Free

• D3S1319E
• PFL
• profilin 2
• Profilin II
• profilin-2

Background

Profilin 2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals by binding to actin (1:1 ratio) and so affecting the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Highly expressed in skeletal muscle, kidney and brain and less strongly in placenta, heart, liver and lung.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFN2