Dynamin Antibody (3G4B6)

Images

 
Genetic Strategies: Knockout Validated: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. ...read more
Simple Western: Dynamin Antibody (3G4B6) [NB110-60491] - Simple Western lane view shows a specific band for Dynamin in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the ...read more
Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).
Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491] - Antibody-bead conjugates were prepared by adding 1.0 ug of antibody to 500 ul of PBS with 0,01% triton X-100 in a microcentrifuge tube and 30ul of protein G ...read more
Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.
Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [NB110-60491] - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using ...read more
Flow Cytometry: Dynamin Antibody (3G4B6) [NB110-60491] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Knockout Validated: Dynamin Antibody (3G4B6) [NB110-60491] - U2OS WT and DNM1 KO cells were labelled with a green or a far red fluorescence dye, respectively. WT and KO cells were mixed and plated to a 1 :1 ratio on ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP, KO
Clone
3G4B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Dynamin Antibody (3G4B6) Summary

Immunogen
Purified recombinant fragment of human Dynamin expressed in E. coli. [UniProt# Q05193]
Localization
Cytoplasm. Cytoplasm > cytoskeleton
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
DNM1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1 ug per million cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200- 1:1000
  • Immunohistochemistry-Paraffin 1:200- 1:1000
  • Immunoprecipitation
  • Knockout Validated
  • Simple Western 1:50
  • Western Blot 1:500- 1:2000
Application Notes
This Dynamin (3G4B6) antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Dynamin Antibody (3G4B6)

  • DNM
  • DNM1
  • dynamin 1
  • Dynamin
  • dynamin-1
  • EC 3.6.5.5

Background

Dynamin (Dynamin-1, Dyn1), with 864-amino acid protein (about 95kDa), belongs to the dynamin family. Dynamin (neuron-specific), Dynamin-2 (ubiquitously expressed), and Dynamin-3 (expressed only in the testis, brain, and lung), constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C. Dynamin is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. Dynamin is also a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that Dynamin gene expression is induced during neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DNM1
Entrez
Uniprot