Perforin Antibody (CB5.4)

Images

 
Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 ...read more
Flow Cytometry: Perforin Antibody (CB5.4) [NBP1-97512] - Analysis of 106 Jurkat cells using Perforin (mouse) Monoclonal Antibody (CB5.4), DyLight488 Conjugate at a concentration of 50 ug/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
CB5.4
Clonality
Monoclonal
Host
Rat
Concentration
1.0 mg/ml

Perforin Antibody (CB5.4) Summary

Immunogen
Recombinant mouse perforin (aa 98-534).
Specificity
Recognizes mouse perforin (region aa 402-534).
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
PRF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This antbody is useful in Immunocytochemistry, Immunohistochemistry (frozen sections, paraffin sections), Immunoprecipitation and Western Blot.
Publications
Read Publications using
NBP1-97512 in the following applications:

Alternate Names for Perforin Antibody (CB5.4)

  • Cytolysin
  • FLH2
  • HPLH2
  • HPLH2lymphocyte pore forming protein
  • Lymphocyte pore-forming protein
  • MGC65093
  • P1PFN1
  • perforin 1 (pore forming protein)
  • perforin-1
  • PFPcytolysin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perforin Antibody (NBP1-97512)(5)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Perforin Antibody (NBP1-97512) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-97512AF405
Alexa Fluor 488 Labeled NBP1-97512AF488
Alexa Fluor 647 Labeled NBP1-97512AF647
Alexa Fluor 700 Labeled NBP1-97512AF700
Allophycocyanin Labeled NBP1-97512APC
DyLight 350 Labeled NBP1-97512UV
DyLight 405 Labeled NBP1-97512V
DyLight 405LS Labeled NBP1-97512V3
DyLight 488 Labeled NBP1-97512G
DyLight 680 Labeled NBP1-97512FR
DyLight 755 Labeled NBP1-97512IR
PE Labeled NBP1-97512PE
PerCP Labeled NBP1-97512PCP

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Blogs on Perforin.

Perforin a Protective Serial Killer
Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Perforin Antibodies for Detecting Immune System Diseases
 Perforin is a calcium-dependent pore forming cytolytic protein. Perforin is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20nm in diameter on target membranes. Killer lymphocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol PRF1

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