Stathmin 1 Antibody - BSA Free

Western Blot: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: Stathmin 1 Antibody [NBP1-76798] - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-76798] - Stathmin 1 antibody was tested in PC12 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-76798] - Human brain tissue with op18 antibody at 2.5 ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, ChHa, Pm
• Applications: WB, ELISA, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Stathmin 1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an C-terminal portion of the human Stathmin 1 protein (between residues 100-149) [UniProt P16949]
• Localization: Cytoplasm
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%), Primate (100%), Chinese Hamster (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STMN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Paraffin 1:100 - 1:250
  • Knockout Validated
  • Western Blot 0.5-1 ug/ml
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Stathmin 1 Peptide (NBP1-76798PEP)

Reactivity Notes

Predicted to react in bovine, primate, pig, Chinese Hamster and canine based on 100% sequence identity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Stathmin 1 Antibody - BSA Free

• C1orf215
• chromosome 1 open reading frame 215
• Lag
• Leukemia-associated phosphoprotein p18
• metablastin
• MGC138869
• Oncoprotein 18
• Op18
• OP18MGC138870
• phosphoprotein 19
• Phosphoprotein p19
• PP17
• PP19
• PR22
• prosolin
• Protein Pr22
• SMNLAP18FLJ32206
• stathmin 1
• stathmin 1/oncoprotein 18
• stathmin
• transmembrane protein C1orf215

Background

Stathmin 1 (STMN1), also known as leukemia-associated phosphoprotein p18, metablastin, oncoprotein 18 (op18), phosphoprotein p19, prosolin, protein Pr22, and pp17, is a member of the stathmin family that encodes a ubiquitous cytosolic phosphoprotein that functions as an intracellular relay for regulatory signals of the cellular environment. STMN1 is involved in the regulation of the microtubule filament system by destabilizing microtubules. In addition, STMN1 has resently been implicated as a potential target of the ASK1-p38 MAP kinase cascade. Phosphorylation at STMN1 Ser-16 may be required for axon formation during neurogenesis. The STMN1 gene has been linked to the control of the learned and innate fear responses. STMN1 is highly expressed in a wide variety of human malignancies, including leukemia, prostate cancer, ovarian carcinoma, and breast carcinoma; suggesting that STMN1 may be an ideal target for anti-cancer therapeutics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Stathmin 1 Antibody (NBP1-76798)(1)

Publication using NBP1-76798

• Bhatia HS, Brunner AD, OztUrk F et al. Spatial proteomics in three-dimensional intact specimens Cell 2022-12-22 [PMID: 36563667]

Bioinformatics

• Gene Symbol: STMN1
• Entrez:
  • 3925(Human)
  • 16765(Mouse)
• Uniprot:
  • P16949()