Stathmin 1 Antibody

Images

 
Genetic Strategies: Knockout Validated: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. ...read more
Western Blot: Stathmin 1 Antibody [NBP1-76798] - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Stathmin 1 Antibody [NBP1-76798] - Stathmin 1 antibody was tested in PC12 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-76798] - Human brain tissue with op18 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, ChHa, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Stathmin 1 Antibody Summary

Immunogen
A synthetic peptide made to an C-terminal portion of the human Stathmin 1 protein (between residues 100-149) [UniProt P16949]
Localization
Cytoplasm
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%), Primate (100%), Chinese Hamster (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STMN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Paraffin 1:100 - 1:250
  • Knockout Validated
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Stathmin 1 Knockout HeLa Cell Lysate
Control Peptide
Stathmin 1 Peptide (NBP1-76798PEP)

Reactivity Notes

Human, mouse and rat. Predicted to react in bovine, primate, pig, Chinese Hamster and canine based on 100% sequence identity.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Stathmin 1 Antibody

  • C1orf215
  • chromosome 1 open reading frame 215
  • Lag
  • Leukemia-associated phosphoprotein p18
  • metablastin
  • MGC138869
  • Oncoprotein 18
  • Op18
  • OP18MGC138870
  • phosphoprotein 19
  • Phosphoprotein p19
  • PP17
  • PP19
  • PR22
  • prosolin
  • Protein Pr22
  • SMNLAP18FLJ32206
  • stathmin 1
  • stathmin 1/oncoprotein 18
  • stathmin
  • transmembrane protein C1orf215

Background

Stathmin 1 (STMN1), also known as leukemia-associated phosphoprotein p18, metablastin, oncoprotein 18 (op18), phosphoprotein p19, prosolin, protein Pr22, and pp17, is a member of the stathmin family that encodes a ubiquitous cytosolic phosphoprotein that functions as an intracellular relay for regulatory signals of the cellular environment. STMN1 is involved in the regulation of the microtubule filament system by destabilizing microtubules. In addition, STMN1 has resently been implicated as a potential target of the ASK1-p38 MAP kinase cascade. Phosphorylation at STMN1 Ser-16 may be required for axon formation during neurogenesis. The STMN1 gene has been linked to the control of the learned and innate fear responses. STMN1 is highly expressed in a wide variety of human malignancies, including leukemia, prostate cancer, ovarian carcinoma, and breast carcinoma; suggesting that STMN1 may be an ideal target for anti-cancer therapeutics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-76798
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Applications: WB, ELISA, ICC/IF, IHC, IHC-P, KO

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Stathmin 1 Antibody (NBP1-76798) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-76798AF350
Alexa Fluor 405 NBP1-76798AF405
Alexa Fluor 488 NBP1-76798AF488
Alexa Fluor 532 NBP1-76798AF532
Alexa Fluor 594 NBP1-76798AF594
Alexa Fluor 647 NBP1-76798AF647
Alexa Fluor 700 NBP1-76798AF700
Alexa Fluor 750 NBP1-76798AF750
Biotin NBP1-76798B
DyLight 350 NBP1-76798UV
DyLight 405 NBP1-76798V
DyLight 488 NBP1-76798G
DyLight 550 NBP1-76798R
DyLight 594 NBP1-76798DL594
DyLight 650 NBP1-76798C
DyLight 680 NBP1-76798FR
DyLight 755 NBP1-76798IR
FITC NBP1-76798F
HRP NBP1-76798H
Janelia Fluor 549 NBP1-76798JF549
Janelia Fluor 646 NBP1-76798JF646

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Bioinformatics

Gene Symbol STMN1
Entrez
Uniprot