RhoA Antibody

Images

 
Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to ...read more
Immunohistochemistry: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to ...read more
Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to ...read more
Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to ...read more
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and ...read more
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and ...read more
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and ...read more
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at ...read more
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on mouse brain (cryo section) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

RhoA Antibody Summary

Immunogen
A synthetic peptide as a part of human, rat, mouse, chicken, dog, bovine, frog, zebrafish, orangutan Transforming protein RhoA (Arha, Arha2) conjugated to an immunogenic carrier protein was used as the immunogen.
Specificity
RhOA.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RHOA
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:300-1:3000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:300-1:3000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
RhoA Lysate (NBL1-15349)
RhoA Lysate (NBP2-65874)
Reviewed Applications
Read 1 Review rated 3
using
NB100-91273 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for RhoA Antibody

  • h12
  • oncogene RHO H12
  • ras homolog gene family, member A
  • Rho cDNA clone 12
  • RHO12
  • RhoA
  • RHOH12ARH12ARHAAplysia ras-related homolog 12
  • small GTP binding protein RhoA
  • transforming protein RhoA

Background

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RhoA Antibody (NB100-91273) (0)

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Review for RhoA Antibody (NB100-91273) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-91273:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot RhoA NB100-91273
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reviewed by:
Anonymous
WB Mouse 05/09/2017
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Summary

ApplicationWestern Blot
Sample Testedmouse embryonic fibroblast cell line, Sample Amount: 40 ug
SpeciesMouse
LotRb0322-2003308-WS

Comments

CommentsMEFs cultured in 10% FBS in DMEM for 48 hours

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RhoA Antibody (NB100-91273) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for RhoA Antibody (NB100-91273)

Discover related pathways, diseases and genes to RhoA Antibody (NB100-91273). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for RhoA Antibody (NB100-91273)

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Pathways for RhoA Antibody (NB100-91273)

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PTMs for RhoA Antibody (NB100-91273)

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Research Areas for RhoA Antibody (NB100-91273)

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HIF-1 beta - activating gene transcription in response to hypoxia
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A good helper on validating your FLOW and IHC data - Rabbit IgG Isotype Control
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Anonymous
05/09/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol RHOA
Entrez
Uniprot