Angiopoietin-like Protein 2/ANGPTL2 Antibody [Unconjugated]

Western blot shows lysates of mouse uterus tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse Angiopoietin‑like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1444) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin‑like Protein 2/ANGPTL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Angiopoietin-like Protein 2/ANGPTL2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Angiopoietin-like 2
  Pro23-His493
  Accession # Q9R045
• Specificity: Detects mouse Angiopoietin-like Protein 2/ANGPTL2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) ANGPTL6 and rmANGPTL1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANGPTL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 2/ANGPTL2 Antibody [Unconjugated]

• angiopoietin-like 2
• Angiopoietin-like Protein 2
• angiopoietin-related protein 2
• ANGPTL2
• ANGRP2
• ARP2HARP
• MGC8889

Background

Angiopoietinlike2 (ANGPTL2), also known as ANGRP2, is a secreted glycoprotein structurally related to Angiopoietin. It does not bind the Tie1 or Tie2 receptors. ANGPTL2 induces sprouting but is not mitogenic in endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-like Protein 2/ANGPTL2 Antibody (AF1444)(2)

Publications using AF1444

• GK Arora, A Gupta, S Narayanan, T Guo, P Iyengar, RE Infante Cachexia-associated adipose loss induced by tumor-secreted leukemia inhibitory factor is counterbalanced by decreased leptin JCI Insight, 2018-07-26;3(14):. 2018-07-26 [PMID: 30046014] (Western Blot, Mouse)
• Kanda A, Noda K, Oike Y, Ishida S Angiopoietin-like protein 2 mediates endotoxin-induced acute inflammation in the eye. Lab Invest, 2012-08-06;92(11):1553-63. 2012-08-06 [PMID: 22868908] (IHC-P, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ANGPTL2
• Uniprot:
  • Q9R045()