Profilin 1 Antibody

Images

 
Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.
Immunohistochemistry: Profilin 1 Antibody [NBP1-19344] - Staining of Profilin in mouse thymus.
Simple Western: Profilin 1 Antibody [NBP1-19344] - Simple Western lane view shows a specific band for Profilin 1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.37 mg/ml

Profilin 1 Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal portion of human Profilin 1 (within residues 100-140). [Swiss-Prot# P07737]
Localization
Cytoplasm, cytoskeleton.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PFN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Simple Western 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This Profilin 1 antibody is useful for Immunohistochemistry on formalin fixed paraffin embedded sections and Western blot, where a band is seen at approx. 14 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Lysate (NB820-59177)
Control Peptide
Profilin 1 Peptide (NBP1-19344PEP)
Publications
Read Publication using
NBP1-19344 in the following applications:

Reactivity Notes

Human, bovine, rat, and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.37 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Profilin 1 Antibody

  • PFN1
  • PROF1
  • Profilin 1
  • Profilin I
  • profilin-1

Background

Profilin-1 (PFN1) is a widely expressed actin-binding protein that implicates in diverse cellular activities ranging from actin polymerization regulation to control of gene transcription. PFN1 facilitate actin polymerization and cell movement by catalyzing the exchange of actin-bound ADP for ATP, and by liberating actin monomer (G-actin) from its sequestering protein, thymosin-beta4; both processes leading to unidirectional addition of G-actin to elongating actin filaments. PFN1 binds actin for impacting cytoskeletal structures, and interestingly, at high concentrations, PFN1 prevents polymerization of actin, and vice versa. PFN1-PIP2 binding inhibits the formation of IP3 and DG, and PFN1 also inhibits AR/HTT aggregation via G-actin binding. Indeed, PFN1 phosphorylation at Ser-138 site has been shown to reduce its affinity for G-actin that follows inhibition of PFN1/HTT interactions and AR/HTT aggregation. PFN1 has long been considered an essential control element for actin polymerization and cell migration, however, recent data suggests an inhibitory action of PFN1 on motility of certain types of carcinoma cells. PFN1 gene disruption causes gross impairment of development and embryonic lethality in insects/mice models abd defects in PFN1 have been linked to amyotrophic lateral sclerosis 18 (ALS18)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Profilin 1 Antibody (NBP1-19344)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Human
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Product General Protocols

View specific protocols for Profilin 1 Antibody (NBP1-19344): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Profilin 1 Antibody (NBP1-19344). (Showing 1 - 1 of 1 FAQ).

  1. We have stained human oral tissue with your PFN1 monoclonal antibody NBP1-19344. Is the antibody specific to PFN1 or does it also bind to PFN2, and if so how well?
    • Unfortunately we have not tested NBP1-19344 against purified PFN2. I have however aligned the immunogen against the human PFN2 protein for you, and found the homology to be just 45%. We usually suggest that a sequence homology of greater than 80% is indicative of likely binding, although without further testing being carried out I cannot say for certain that this antibody would not recognise PFN2.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-19344AF405
Alexa Fluor 488 Labeled NBP1-19344AF488
Alexa Fluor 647 Labeled NBP1-19344AF647
Alexa Fluor 700 Labeled NBP1-19344AF700
Biotin Labeled NBP1-19344B
DyLight 405 Labeled NBP1-19344V
DyLight 488 Labeled NBP1-19344G
DyLight 550 Labeled NBP1-19344R
DyLight 650 Labeled NBP1-19344C
FITC Labeled NBP1-19344F
HRP Labeled NBP1-19344H

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Blogs on Profilin 1.

Profiling the Profilin 1 Antibody
Profilin-1, or Pfn-1, is a small actin-binding protein which plays an essential role controlling the growth of microfilaments. Profilin 1 and Profilin 2 have similar biochemical properties but are expressed in different tissues. The Profilin 1 antibod...  Read full blog post.

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Bioinformatics

Gene Symbol PFN1
Entrez