Profilin 2 Antibody

Images

 
Western Blot: Profilin 2 Antibody [NBP1-31629] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 2 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-31629] - Profilin 2 antibody detects PFN2 protein at cytoplasm on human colon carcinoma.
Western Blot: Profilin 2 Antibody [NBP1-31629] - Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Profilin 2 Antibody [NBP1-31629] - Sample(30 ug whole cell lysate)A: A431 B: H1299 12% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Profilin 2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Profilin 2. The exact sequence is proprietary.
Localization
Cytoplasm, cytoskeleton
Predicted Species
Bovine (92%), Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PFN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Profilin 2 Antibody

  • D3S1319E
  • PFL
  • profilin 2
  • Profilin II
  • profilin-2

Background

Profilin 2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals by binding to actin (1:1 ratio) and so affecting the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Highly expressed in skeletal muscle, kidney and brain and less strongly in placenta, heart, liver and lung.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to Profilin 2 Antibody (NBP1-31629). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Profilin 2.

Profiling the Profilin 1 Antibody
Profilin-1, or Pfn-1, is a small actin-binding protein which plays an essential role controlling the growth of microfilaments. Profilin 1 and Profilin 2 have similar biochemical properties but are expressed in different tissues. The Profilin 1 antibod...  Read full blog post.

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Bioinformatics

Gene Symbol PFN2