DIAPH1 Antibody

Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.66 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Immunohistochemistry: DIAPH1 Antibody [NB600-231] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Diaphanous used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB

Western Blot: DIAPH1 Antibody [NB600-231] - Whole cell lysate (50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 ug/ml.

Immunohistochemistry-Paraffin: DIAPH1 Antibody [NB600-231] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-Diaphanous1 used at a dilution of 1:100.

• Reactivity: Hu
• Applications: WB, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

DIAPH1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1200 and the C-terminus (residue 1248) of human Diaphanous Homolog 1 using the numbering given in entry NP_005210.1 (GeneID 1729).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DIAPH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Knockdown Validated
  • Western Blot 1:5000-1:15000
• Application Notes: By Western Blot this antibody recognizes a band at ~205 kDa, representing Diaphanous 1. Epitope Retrieval is not recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DIAPH1 Antibody

• DIA1
• diaphanous homolog 1 (Drosophila)
• hDIA1
• homolog) 1
• LFHL1FLJ25265

Background

Diaphanous 1 is a member of the Formin homology (FH) family of cytoskeletal organizer proteins. It is required for cytokinesis and transcriptional activation of the serum response factor. It has also been suggested to contribute to localized actin polymerization and stress fiber formation, both by recruiting profiling to sites of Rho action and by cooperating with ROCK. The human Diaphanous 1 gene is associated with Premature Ovarian Failure (POF) and Nonsyndromic Deafness (DFNA1), an autosomal dominant, fully penetrant progressive hearing loss.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DIAPH1 Antibody (NB600-231)(2)

Publications using NB600-231

• Lartey J, Smith M, Pawade J et al. Up-regulation of myometrial RHO effector proteins (PKN1 and DIAPH1) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor. Biol Reprod 2007-06-01 [PMID: 17301291]
• Jewett TJ, Fischer ER, Mead DJ et al. Chlamydial TARP is a bacterial nucleator of actin. Proc Natl Acad Sci U S A 2006-10-01 [PMID: 17028176]

Bioinformatics

• Gene Symbol: DIAPH1
• Entrez:
  • 1729(Human)
• Uniprot:
  • O60610(Human)