Hu,
Mu,
Rt,
Bv,
Ca,
HaSpecies Glossary
Reactivity: Hu, Mu, Rt, Bv, Ca, Ha
WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody (3B6-PPAR)-Western Blot-NB300-537-img0006.jpg "Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.")
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Flow-Cytometry-NB300-537-img0005.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Western-Blot-NB300-537-img0002.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Flow-Cytometry-NB300-537-img0003.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Flow-Cytometry-NB300-537-img0004.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Western-Blot-NB600-636-img0014.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NB600-636] - Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary antibody only with NIH/3T3 cells. Anti-PPAR alpha antibody was used at 10 ug/mL, 1h at RT degrees. Secondary antibody: Anti-RABBIT IgG DyLight (R) 488 Conjugated Preadsorbed at 5 ug/ml for 1 h at RT. Staining: PPAR as green fluorescent signal with DAPI (blue) nuclear counterstain.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-636-img0021.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NB600-636] - Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventricle (exit point of progenitor olfactory neurons); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunohistochemistry-Paraffin-NB600-636-img0007.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Lane 1: NIH/3T3. Load: 10 ug per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 30 min. Predicted/Observed size: 50 kDa for PPAR Alpha.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody-Western Blot-NB600-636-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NB600-636] - Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 ug/mL overnight at 4C. Secondary antibody: Alexa Fluor 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-636-img0012.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NB600-636] - Mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunohistochemistry-Paraffin-NB600-636-img0006.jpg)
![ELISA: PPAR alpha/NR1C1 Antibody [NB600-636] - ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-ELISA-NB600-636-img0019.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-03288] - Affinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kD band corresponding to PPAR alpha present in a 3T3 whole cell lysate.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Western-Blot-NBP1-03288-img0006.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-03288] - Affinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kD band corresponding to PPAR alpha present in a 3T3 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NBP1-03288] - PPAR alpha Antibody [NBP1-03288] - Staining of human primary macrophages using NBP1-03288 at a dilution of 1/100. Photo courtesy of a product review by verified customer.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03288-img0007.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NBP1-03288] - PPAR alpha Antibody [NBP1-03288] - Immunohistochemistry using anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunohistochemistry-Paraffin-NBP1-03288-img0008.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NBP1-03288] - Staining of human liver.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunohistochemistry-Paraffin-NBP1-03288-img0005.jpg)
![Western Blot: PPAR alpha Antibody [NBP1-31834] - Sample (30 ug of whole cell lysate) A: Rat primary hepatocyte 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/PPAR alpha Antibody-Western Blot-NBP1-31834-img0002.jpg "Western Blot: PPAR alpha Antibody [NBP1-31834] - Sample (30 ug of whole cell lysate) A: Rat primary hepatocyte 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Chromatin Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Chromatin-Immunoprecipitation-(ChIP)-NBP1-31834-img0006.jpg)
![Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 ug of PPAR alpha antibody 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunoprecipitation-NBP1-31834-img0004.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (1E8) [NBP2-01211] - analysis of PPAR alpha in HeLa cell lysate (25ug) using anti-PPAR alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(1E8)-Western-Blot-NBP2-01211-img0004.jpg "Western Blot: PPAR alpha/NR1C1 Antibody (1E8) [NBP2-01211] - analysis of PPAR alpha in HeLa cell lysate (25ug) using anti-PPAR alpha antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha Antibody (1E8) [NBP2-01211] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPAR alpha.](http://images.novusbio.com/fullsize/PPAR alpha Antibody (1E8)-Immunocytochemistry-Immunofluorescence-NBP2-01211-img0002.jpg)
![Western Blot: PPAR alpha Antibody (1E8) [NBP2-01211] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAR alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAR alpha.](http://images.novusbio.com/fullsize/PPAR alpha Antibody (1E8)-Western Blot-NBP2-01211-img0001.jpg)
![Western Blot: PPAR alpha Antibody (1E8) [NBP2-01211] Analysis of extracts (35ug) from 9 different cell lines by using anti-PPAR alpha monoclonal antibody.](http://images.novusbio.com/fullsize/PPAR alpha Antibody (1E8)-Western Blot-NBP2-01211-img0003.jpg)
![Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 [p Ser12] Antibody-Western Blot-NB120-3484-img0004.jpg "Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3484-img0003.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of PPAR alpha [pSer12] was done on MCF7 cells.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Flow-Cytometry-NB120-3484-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of U-87 MG cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3484-img0002.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(5D10C3)-Western-Blot-NBP2-52538-img0002.jpg "Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)")
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(5D10C3)-Flow-Cytometry-NBP2-52538-img0001.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody (5D10C3)-Western Blot-NBP2-52538-img0003.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10E10) [NBP2-52537] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(5D10E10)-Western-Blot-NBP2-52537-img0002.jpg "Western Blot: PPAR alpha/NR1C1 Antibody (5D10E10) [NBP2-52537] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)")
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (5D10E10) [NBP2-52537] - Analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(5D10E10)-Flow-Cytometry-NBP2-52537-img0001.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10E10) [NBP2-52537] - Analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody (5D10E10)-Western Blot-NBP2-52537-img0003.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NBP2-57777] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody-Western Blot-NBP2-57777-img0002.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NBP2-57777] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NBP2-57777] - Staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57777-img0001.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [H00005465-B01P] - Analysis of PPARA expression in transfected 293T cell line by PPARA polyclonal antibody. Lane 1: PPARA transfected lysate(51.48 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody-Western Blot-H00005465-B01P-img0004.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [H00005465-B01P] - Analysis of PPARA expression in transfected 293T cell line by PPARA polyclonal antibody. Lane 1: PPARA transfected lysate(51.48 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [H00005465-B01P] - Analysis of purified antibody to PPARA on HepG2 cell. (antibody concentration 20 ug/ml)](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunocytochemistry-Immunofluorescence-H00005465-B01P-img0003.jpg)
