Skip to main content
Novus Biologicals uses cookies to provide you with a great website experience. By continuing to use this website you acknowledge this and agree to our cookie policy.
OK
Learn More
Support: 1-888-506-6887
Items in Cart (
0
)
[X]
Quick Order
Account
Our Guarantee
Antibodies
Primary Antibodies
Conjugated Antibodies
Sample Size Antibodies
KO Validated Antibodies
Antibody Sampler Packs
Antibody Pairs
Secondary Antibodies
Isotype Control Antibodies
Proteins and Peptides
Peptides & Proteins
Blocking Peptides
Biologically Active Proteins
Lysates and Cell Lines
Lysates
Knockout 293T Cell Lysates
Knockout HeLa Cell Lysates
Reporter Cell Lines
Stem Cell Lines
Kits
Antibody Labeling Kits
ChIP Kits
ELISA Kits
Flow Cytometry Kits
Exosome Kits
Activators and Inhibitors
Inhibitors
Activators
Support Products
IHC Tissue Slides
IHC Microarrays
Western Blot Gels & Buffers
Western Blot Membranes
RNAi
All Support Products
Bulk and Custom
Products
Our Protocols
Resources
Protocols/Troubleshooting Guides
Posters and Literature
Calculators
FAQs
Blogs
Diseases
Whitepapers
Antibody Concierge
Tools
Flow Cytometry Panel Builder
Laverne - Bioinformatics Tool
Application
Western Blotting
ICC
IHC
ELISA
Interviews
Faculty
Postdoc
Resources
View Pathways
By Research Area
Apoptosis
Autophagy
Cancer
Cell Biology
Cellular Markers
Epigenetics
Epitope Tags
Hypoxia
Immunology
Neuroscience
Stem Cells
By Pathway
AKT
Apoptosis
Autophagy
Hedgehog
Hippo
MAPK/ERK
Necroptosis
NFKB Signaling
p38 MAPK
TGF Beta
TLR
Research Areas
Get Rewards
Customer Care
100% Guarantee
Risk Free Testing
Novus Rewards Program
Publication Rewards Program
New Lab Discount
Scholarship
Travel Grants
Promotions
Customer Care
Novus Careers
About us
Careers
Collaborations
Events
Novus Story
Our Team
Philanthropy
Press Releases
Why choose Novus
About Us
Contact Us
Customer Service
Distributors
Technical Support
Contact
PPAR alpha/NR1C1
Filter Results
Clear All
clear
Category
Primary Antibodies
(11)
RNAi
(5)
Lysates
(3)
Peptides and Proteins
(3)
Kits
(1)
clear
Protein/Target
PPAR alpha / NR1C1
(23)
clear
Applications
Western Blot
(14)
Immunocytochemistry / Immunofluorescence
(8)
ELISA
(6)
Flow Cytometry
(5)
-------------- All A - Z ---------------
Affinity Purification
(1)
Antibody Competition
(2)
Chromatin Immunoprecipitation
(4)
CyTOF-ready
(2)
ELISA
(6)
Flow Cytometry
(5)
Functional
(1)
Gel Shift
(1)
Gel Super Shift Assays
(1)
Immunocytochemistry / Immunofluorescence
(2)
Immunocytochemistry / Immunofluorescence
(8)
Immunohistochemistry
(5)
Immunohistochemistry-Paraffin
(3)
Immunoprecipitation
(3)
Protein Array
(1)
RNA Inhibition
(5)
RNAi sequence position
(5)
Western Blot
(14)
clear
Reactivity
Human
(22)
Mouse
(7)
Rat
(5)
Bovine
(3)
-------------- All A - Z ---------------
Bovine
(3)
Canine
(3)
Hamster
(2)
Human
(22)
Insect
(1)
Monkey
(1)
Mouse
(7)
Porcine
(2)
Rabbit
(1)
Rat
(5)
Rhesus Macaque
(1)
clear
Citation
1 or more (6)
clear
Reviews
5 (2)
clear
Host
Rabbit
(6)
Mouse
(5)
clear
Available Conjugates
Unconjugated
(15)
Alexa Fluor 405
(1)
Alexa Fluor 488
(1)
Alexa Fluor 647
(1)
-------------- All A - Z ---------------
Alexa Fluor 405
(1)
Alexa Fluor 488
(1)
Alexa Fluor 647
(1)
Alexa Fluor 700
(1)
Allophycocyanin
(1)
Biotin
(1)
DyLight 350
(1)
DyLight 405
(1)
DyLight 488
(1)
DyLight 550
(1)
DyLight 650
(1)
DyLight 680
(1)
DyLight 755
(1)
FITC
(1)
HRP
(1)
Janelia Fluor 549
(1)
Janelia Fluor 646
(1)
PE
(1)
PerCP
(1)
Unconjugated
(15)
clear
Format
Azide and BSA Free
(1)
clear
Modification
p Ser12
(1)
clear
Clonality
Polyclonal
(6)
Monoclonal
(5)
clear
Clone
3B6 / PPAR
(1)
5D10C3
(1)
5D10E10
(1)
H0723
(1)
OTI1E8
(1)
clear
Related Diseases
Atherosclerosis
(23)
Cardiovascular Diseases
(23)
Coronary Artery Disease
(23)
Diabetes Mellitus
(23)
-------------- All A - Z ---------------
Atherosclerosis
(23)
Cardiovascular Diseases
(23)
Coronary Artery Disease
(23)
Diabetes Mellitus
(23)
Diabetes Mellitus, Non-insulin-dependent
(23)
Dyslipidemias
(23)
Fatty Liver
(23)
Heart Diseases
(23)
Hypertensive Disease
(23)
Hypertrophy
(23)
Inflammation
(23)
Insulin Resistance
(23)
Insulin Sensitivity
(23)
Malignant Neoplasms
(23)
Myocardial Ischemia
(23)
Neoplasms
(23)
Obesity
(23)
Steatohepatitis
(23)
Steatosis
(23)
Tissue Adhesions
(23)
clear
Images Type
Western Blot
(12)
Immunocytochemistry / Immunofluorescence
(6)
Flow Cytometry
(4)
Immunohistochemistry-Paraffin
(2)
-------------- All A - Z ---------------
Western Blot
(12)
Immunocytochemistry / Immunofluorescence
(6)
Flow Cytometry
(4)
Immunohistochemistry-Paraffin
(2)
Chromatin Immunoprecipitation
(1)
ELISA
(1)
Immunoprecipitation
(1)
clear
Isotype
IgG
(6)
IgG1
(3)
IgG2a
(1)
IgG2b
(1)
clear
Related Pathways
Aging
(23)
Cell Activation
(23)
Cell Adhesion
(23)
Cell Differentiation
(23)
-------------- All A - Z ---------------
Aging
(23)
Cell Activation
(23)
Cell Adhesion
(23)
Cell Differentiation
(23)
Cell Proliferation
(23)
Energy Homeostasis
(23)
Fatty Acid Beta-oxidation
(23)
Fatty Acid Oxidation
(23)
Fatty Acid Transport
(23)
Glucose Homeostasis
(23)
Immune Response
(23)
Inflammatory Response
(23)
Methylation
(23)
Parturition
(23)
Pathogenesis
(23)
Response To Fenofibrate
(23)
Secretion
(23)
Tissue Development
(23)
Translation
(23)
Transport
(23)
clear
Preservative
0.05% Sodium Azide
(4)
0.02% Sodium Azide
(3)
0.01% Sodium Azide
(2)
No Preservative
(2)
0.01% Thimerosal
(1)
0.1% Sodium Azide
(1)
clear
Protein Or Peptide
Recombinant Protein Antigen
(2)
Recombinant Protein
(1)
clear
Research Areas
mTOR Pathway
(22)
Lipid and Metabolism
(19)
Transcription Factors and Regulators
(19)
Cancer
(14)
Phospho Specific
(3)
clear
Has Sample Size
No
(20)
Yes
(3)
clear
Promotional Item
No
(23)
Sort By:
Best Match
Products (A-Z)
Newest
Publications
Species Glossary
At
=
A. thaliana
SyHa
=
Golden Syrian Hamster
All
=
All Species
Ha
=
Hamster
An
=
Animal
Hu
=
Human
ArHa
=
Armenian Hamster
I
=
Insect
Av
=
Avian
Ll
=
Llama
Bb
=
Baboon
Ma
=
Mammal
Ba
=
Bacteria
Mk
=
Monkey
Bv
=
Bovine
Mu
=
Mouse
Ca
=
Canine
Multi
=
Multi-species
Ce
=
C. Elegans
NA
=
Non-species specific
Ch
=
Chicken
Or
=
Orangutan
ChHa
=
Chinese Hamster
Pl
=
Plant
Cm
=
Cynomolgus Monkey
Pm
=
Primate
Do
=
Donkey
Po
=
Porcine
Dr
=
Drosophilia
Rb
=
Rabbit
Ec
=
E. Coli
Rt
=
Rat
Eq
=
Equine
RM
=
Rhesus Macaque
Fe
=
Feline
Sh
=
Sheep
Ft
=
Ferret
Vb
=
Vertebrate
Fi
=
Fish
Vi
=
Virus
Fu
=
Fungi
Xp
=
Xenopus
Ge
=
Gerbil
Ye
=
Yeast
GP
=
Guinea Pig
Ze
=
Zebrafish
Gt
=
Goat
Note: Mouseover a species abbreviation on the product page to display the fullname.
Products (23)
Full Site
page
of
2
PPARA Products By Gene Name
23 Results for "PPARA"
List
Grid
NB300-537
PPAR alpha/NR1C1 Antibody (3B6/PPAR)
(1 Review)
(9 Publications)
100 μg
NB300-537
Reactivity:
Hu
,
Mu
,
Rt
,
Bv
,
Rb
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Bv
,
Rb
Applications:
WB
,
ChIP
,
Flow
,
GS
,
ICC/IF
,
IHC
,
IHC-P
,
IP
Applications:
WB
,
ChIP
,
Flow
,
GS
,
ICC/IF
,
IHC
,
IHC-P
,
IP
Host:
Mouse Monoclonal
Host:
Mouse Monoclonal
Conjugate:
Unconjugated
Available Conjugates:
Conjugates
Conjugate
Catalog #
Availability
Size
Price
Alexa Fluor 405
NB300-537AF405
Alexa Fluor 488
NB300-537AF488
Alexa Fluor 647
NB300-537AF647
Alexa Fluor 700
NB300-537AF700
Allophycocyanin
NB300-537APC
Biotin
NB300-537B
DyLight 350
NB300-537UV
DyLight 405
NB300-537V
DyLight 488
NB300-537G
DyLight 550
NB300-537R
DyLight 650
NB300-537C
DyLight 680
NB300-537FR
DyLight 755
NB300-537IR
FITC
NB300-537F
HRP
NB300-537H
Janelia Fluor 549
NB300-537JF549
Janelia Fluor 646
NB300-537JF646
PE
NB300-537PE
PerCP
NB300-537PCP
compare
(select up to 3 products from this page to compare)
NB600-636
PPAR alpha/NR1C1 Antibody
(6 Publications)
0.1 mg
NB600-636
Reactivity:
Hu
,
Mu
,
Rt
,
Bv
,
Ca
,
Ha
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Bv
,
Ca
,
Ha
Applications:
WB
,
ChIP
,
ELISA
,
Flow
,
ICC/IF
,
IHC
,
IHC-P
Applications:
WB
,
ChIP
,
ELISA
,
Flow
,
ICC/IF
,
IHC
,
IHC-P
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
NBP1-03288
PPAR alpha/NR1C1 Antibody
(2 Reviews)
(2 Publications)
0.05 mg
NBP1-03288
Reactivity:
Hu
,
Mu
,
Rt
,
Po
,
Bv
,
Ca
,
Ha
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Po
,
Bv
,
Ca
,
Ha
Applications:
WB
,
ELISA
,
ICC/IF
,
IHC
,
IHC-P
Applications:
WB
,
ELISA
,
ICC/IF
,
IHC
,
IHC-P
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
PP-H0723-00
PPAR alpha/NR1C1 Antibody (H0723) [Unconjugated]
(3 Publications)
100 μg
Reactivity:
Hu
Species Glossary
Reactivity:
Hu
Applications:
WB
,
IP
,
ChIP
,
ELISA
,
GS
Applications:
WB
,
IP
,
ChIP
,
ELISA
,
GS
Host:
Mouse Monoclonal
Host:
Mouse Monoclonal
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
NBP1-31834
PPAR alpha/NR1C1 Antibody
0.1 ml
NBP1-31834
Reactivity:
Hu
,
Mu
,
Rt
,
I
,
Po
,
Ca
,
RM
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
I
,
Po
,
Ca
,
RM
Applications:
WB
,
ChIP
,
ICC/IF
,
IP
,
ChIP
Applications:
WB
,
ChIP
,
ICC/IF
,
IP
,
ChIP
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
NBP2-01211
PPAR alpha/NR1C1 Antibody (OTI1E8)
0.1 ml
NBP2-01211
Reactivity:
Hu
,
Mu
Species Glossary
Reactivity:
Hu
,
Mu
Applications:
WB
,
ICC/IF
Applications:
WB
,
ICC/IF
Host:
Mouse Monoclonal
Host:
Mouse Monoclonal
Conjugate:
Unconjugated
Available Formulations
Formulations
Formulation
Catalog #
Availability
Price
Azide and BSA Free
NBP2-73567
compare
(select up to 3 products from this page to compare)
NBP2-52538
PPAR alpha/NR1C1 Antibody (5D10C3)
0.1 mg
NBP2-52538
Reactivity:
Hu
,
Mk
Species Glossary
Reactivity:
Hu
,
Mk
Applications:
WB
,
ELISA
,
Flow
,
IHC
,
CyTOF-ready
,
ICC/IF
Applications:
WB
,
ELISA
,
Flow
,
IHC
,
CyTOF-ready
,
ICC/IF
Host:
Mouse Monoclonal
Host:
Mouse Monoclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
NB120-3484
PPAR alpha/NR1C1 [p Ser12] Antibody
100μg
NB120-3484
Reactivity:
Hu
,
Mu
Species Glossary
Reactivity:
Hu
,
Mu
Applications:
WB
,
Flow
,
ICC/IF
Applications:
WB
,
Flow
,
ICC/IF
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
NBP2-52537
PPAR alpha/NR1C1 Antibody (5D10E10)
0.025 mg
NBP2-52537
Reactivity:
Hu
Species Glossary
Reactivity:
Hu
Applications:
WB
,
ELISA
,
Flow
,
IHC
,
CyTOF-ready
,
ICC/IF
Applications:
WB
,
ELISA
,
Flow
,
IHC
,
CyTOF-ready
,
ICC/IF
Host:
Mouse Monoclonal
Host:
Mouse Monoclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
NBP2-57777
PPAR alpha/NR1C1 Antibody
100 ul
NBP2-57777
Reactivity:
Hu
Species Glossary
Reactivity:
Hu
Applications:
WB
,
ICC/IF
Applications:
WB
,
ICC/IF
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
H00005465-P01
Recombinant Human PPAR alpha/NR1C1 Protein
(1 Publication)
10 μg
H00005465-P01
Reactivity:
Hu
Species Glossary
Reactivity:
Hu
Applications:
WB
,
ELISA
,
PA
,
AP
Applications:
WB
,
ELISA
,
PA
,
AP
compare
(select up to 3 products from this page to compare)
NBP2-58507
PPAR alpha/NR1C1 Antibody
100 ul
NBP2-58507
Reactivity:
Hu
,
Mu
,
Rt
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
Applications:
ICC/IF
Applications:
ICC/IF
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
page
of
2
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Western-Blot-NB300-537-img0006.jpg "Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.")
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Flow-Cytometry-NB300-537-img0007.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Western-Blot-NB300-537-img0002.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Flow-Cytometry-NB300-537-img0003.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Flow-Cytometry-NB300-537-img0004.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Flow-Cytometry-NB300-537-img0005.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Western-Blot-NB600-636-img0014.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NB600-636] - Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary antibody only with NIH/3T3 cells. Anti-PPAR alpha antibody was used at 10 ug/mL, 1h at RT degrees. Secondary antibody: Anti-RABBIT IgG DyLight (R) 488 Conjugated Preadsorbed at 5 ug/ml for 1 h at RT. Staining: PPAR as green fluorescent signal with DAPI (blue) nuclear counterstain.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-636-img0021.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NB600-636] - Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventricle (exit point of progenitor olfactory neurons); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunohistochemistry-Paraffin-NB600-636-img0007.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Lane 1: NIH/3T3. Load: 10 ug per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 30 min. Predicted/Observed size: 50 kDa for PPAR Alpha.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody-Western Blot-NB600-636-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NB600-636] - Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 ug/mL overnight at 4C. Secondary antibody: Alexa Fluor 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-636-img0012.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NB600-636] - Mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunohistochemistry-Paraffin-NB600-636-img0006.jpg)
![ELISA: PPAR alpha/NR1C1 Antibody [NB600-636] - ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-ELISA-NB600-636-img0019.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-03288] - Affinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kD band corresponding to PPAR alpha present in a 3T3 whole cell lysate.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Western-Blot-NBP1-03288-img0006.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-03288] - Affinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kD band corresponding to PPAR alpha present in a 3T3 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NBP1-03288] - PPAR alpha Antibody [NBP1-03288] - Staining of human primary macrophages using NBP1-03288 at a dilution of 1/100. Photo courtesy of a product review by verified customer.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03288-img0007.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NBP1-03288] - PPAR alpha Antibody [NBP1-03288] - Immunohistochemistry using anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunohistochemistry-Paraffin-NBP1-03288-img0008.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NBP1-03288] - Staining of human liver.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunohistochemistry-Paraffin-NBP1-03288-img0005.jpg)
![Western Blot: PPAR alpha Antibody [NBP1-31834] - Sample (30 ug of whole cell lysate) A: Rat primary hepatocyte 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/PPAR alpha Antibody-Western Blot-NBP1-31834-img0002.jpg "Western Blot: PPAR alpha Antibody [NBP1-31834] - Sample (30 ug of whole cell lysate) A: Rat primary hepatocyte 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Chromatin Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Chromatin-Immunoprecipitation-(ChIP)-NBP1-31834-img0006.jpg)
![Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 ug of PPAR alpha antibody 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunoprecipitation-NBP1-31834-img0004.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (1E8) [NBP2-01211] - analysis of PPAR alpha in HeLa cell lysate (25ug) using anti-PPAR alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(1E8)-Western-Blot-NBP2-01211-img0004.jpg "Western Blot: PPAR alpha/NR1C1 Antibody (1E8) [NBP2-01211] - analysis of PPAR alpha in HeLa cell lysate (25ug) using anti-PPAR alpha antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha Antibody (1E8) [NBP2-01211] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPAR alpha.](http://images.novusbio.com/fullsize/PPAR alpha Antibody (1E8)-Immunocytochemistry-Immunofluorescence-NBP2-01211-img0002.jpg)
![Western Blot: PPAR alpha Antibody (1E8) [NBP2-01211] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAR alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAR alpha.](http://images.novusbio.com/fullsize/PPAR alpha Antibody (1E8)-Western Blot-NBP2-01211-img0001.jpg)
![Western Blot: PPAR alpha Antibody (1E8) [NBP2-01211] Analysis of extracts (35ug) from 9 different cell lines by using anti-PPAR alpha monoclonal antibody.](http://images.novusbio.com/fullsize/PPAR alpha Antibody (1E8)-Western Blot-NBP2-01211-img0003.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(5D10C3)-Western-Blot-NBP2-52538-img0002.jpg "Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)")
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(5D10C3)-Flow-Cytometry-NBP2-52538-img0001.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody (5D10C3)-Western Blot-NBP2-52538-img0003.jpg)
![Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 [p Ser12] Antibody-Western Blot-NB120-3484-img0004.jpg "Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3484-img0003.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of PPAR alpha [pSer12] was done on MCF7 cells.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Flow-Cytometry-NB120-3484-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of U-87 MG cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3484-img0002.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10E10) [NBP2-52537] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(5D10E10)-Western-Blot-NBP2-52537-img0002.jpg "Western Blot: PPAR alpha/NR1C1 Antibody (5D10E10) [NBP2-52537] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)")
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (5D10E10) [NBP2-52537] - Analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(5D10E10)-Flow-Cytometry-NBP2-52537-img0001.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10E10) [NBP2-52537] - Analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody (5D10E10)-Western Blot-NBP2-52537-img0003.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NBP2-57777] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody-Western Blot-NBP2-57777-img0002.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NBP2-57777] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NBP2-57777] - Staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57777-img0001.jpg)
![Recombinant Human PPAR alpha/NR1C1 Protein [H00005465-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005465-P01-1.jpg "Recombinant Human PPAR alpha/NR1C1 Protein [H00005465-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NBP2-58507] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/PPAR alpha-NR1C1 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58507-img0001.jpg "Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NBP2-58507] - Staining of human cell line A-431 shows localization to nucleoplasm.")