Podocalyxin Like Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Podocalyxin Like Antibody [Biotin] Summary

Immunogen
A synthetic peptide within an internal region (residues 100-200) of the human Podocalyxin protein. [Swiss-Prot# O00592] This immunogen should be far enough away from any glycosylation site.
Localization
Membrane
Marker
Podocytes /Apical Marker, Endothelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PODXL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Podocalyxin antibody is useful for Immunocytochemistry/Immunofluorescence, Flow Cytometry and Western blot analysis. In WB a band is observed at ~52 kDa. In ICC/IF membrane staining was observed.
Readout System

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Podocalyxin Like Antibody [Biotin]

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like
  • PODXL

Background

Podocalyxin is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiologic role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB110-41503G
DyLight 550 Labeled NB110-41503R
DyLight 650 Labeled NB110-41503C
HRP Labeled NB110-41503H

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Bioinformatics

Gene Symbol PODXL
Entrez