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Podocalyxin Like Antibody [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-41503AF350
Alexa Fluor 405
NB110-41503AF405
Alexa Fluor 488
NB110-41503AF488
Alexa Fluor 532
NB110-41503AF532
Alexa Fluor 594
NB110-41503AF594
Alexa Fluor 647
NB110-41503AF647
Alexa Fluor 700
NB110-41503AF700
Alexa Fluor 750
NB110-41503AF750
Allophycocyanin/Cy7
NB110-41503APCCY7
Biotin
NB110-41503B
DyLight 350
NB110-41503UV
DyLight 405
NB110-41503V
DyLight 488
NB110-41503G
DyLight 550
NB110-41503R
DyLight 594
NB110-41503DL594
DyLight 650
NB110-41503C
DyLight 680
NB110-41503FR
DyLight 755
NB110-41503IR
FITC
NB110-41503F
HRP
NB110-41503H
Janelia Fluor 549
NB110-41503JF549
PE
NB110-41503PE
PE/Atto594
NB110-41503PEATT594
PE/Cy5.5
NB110-41503PECY55
PE/Cy7
NB110-41503PECY7
Unconjugated
NB110-41503
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Podocalyxin Like Antibody [Janelia Fluor® 646] Summary

Immunogen
A synthetic peptide within an internal region (residues 100-200) of the human Podocalyxin protein. [Swiss-Prot: O00592] This immunogen should be far enough away from any glycosylation site.
Localization
Membrane
Marker
Podocytes /Apical Marker, Endothelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PODXL
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Flow (Intracellular)
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Podocalyxin Like Antibody [Janelia Fluor® 646]

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like
  • PODXL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: HLA DRB4 Antibody (4C8) [H00003126-M01] - Analysis of monoclonal antibody to HLA-DRB4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.
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TNF-alpha
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
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Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM288).
Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DRB1 Antibody (SPM288).
Flow Cytometry: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (SPM288).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Reductase/DHFR Antibo ...
H00001719-M01
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Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Analysis of monoclonal antibody to DHFR on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - DHFR monoclonal antibody (M01), clone 2B10 Analysis of DHFR expression in HeLa.
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Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
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Nephrin Antibody
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AF3159
Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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HLA B Antibody
(2G7B10)
NBP2-61871
Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.
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CD34 Antibody (MEC
14.7)
NB600-1071
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FABP8 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Human FABP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5866) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunocytochemistry/Immunofluorescence: RPLP2 Antibody [NBP2-15104] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
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HLA DRB5 Antibody
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Western Blot: HLA DRB5 Antibody [NBP2-14093] - Analysis in human cell line HDLM-2.
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Cytochrome c
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Caspase-3 Antibody
(31A1067) - (Pro and ...
NB100-56708
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.
Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
(1 Review)
(153 Publications)
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
(1 Review)
(6 Publications)
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Applications: WB, IHC, IHC-P
Podocalyxin Like
Antibody [Janelia Flu ...
NB110-41503JF646
Podocalyxin Like Antibody [Janelia Fluor® 646]
Species: Hu, Mu
Applications: WB, Flow, Flow-IC, ICC/IF

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-41503AF350
Alexa Fluor 405 NB110-41503AF405
Alexa Fluor 488 NB110-41503AF488
Alexa Fluor 532 NB110-41503AF532
Alexa Fluor 594 NB110-41503AF594
Alexa Fluor 647 NB110-41503AF647
Alexa Fluor 700 NB110-41503AF700
Alexa Fluor 750 NB110-41503AF750
Allophycocyanin/Cy7 NB110-41503APCCY7
Biotin NB110-41503B
DyLight 350 NB110-41503UV
DyLight 405 NB110-41503V
DyLight 488 NB110-41503G
DyLight 550 NB110-41503R
DyLight 594 NB110-41503DL594
DyLight 650 NB110-41503C
DyLight 680 NB110-41503FR
DyLight 755 NB110-41503IR
FITC NB110-41503F
HRP NB110-41503H
Janelia Fluor 549 NB110-41503JF549
Janelia Fluor 646 NB110-41503JF646
PE NB110-41503PE
PE/Atto594 NB110-41503PEATT594
PE/Cy5.5 NB110-41503PECY55
PE/Cy7 NB110-41503PECY7
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol PODXL