Podocalyxin Like Antibodies: Novus Biologicals

AF1556

Podocalyxin Like Antibody [Unconjugated]

(3 Reviews)

100 μg

AF1556

HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Defects in TRAF3IP1 mutants are mediated by MAP4.(a) Lateral views of WT zebrafish embryos injected with map4 morpholino at 48 hpf and phenotype distribution in WT embryos injected with control or map4 morpholino. (b) Lateral views of elipsa zebrafish embryos injected with map4 morpholino at 48 hpf and phenotype distribution in elipsa mutant embryos injected with control or map4 morpholino (data shown as combined result of n=3 independent experiments). Scale bars, 1 mm. (c) Relative expression of Map4 normalized to that of Hprt was analysed by qPCR in control and Traf3ip1-KD mIMCD3 cells stably expressing GFP or GFP-IFT54 mutants and Map4 shRNA. (d) Control and Traf3ip1-KD/ Map4-KD mIMCD3 cells expressing either GFP or IFT54-GFP fusions were fixed in MeOH and stained for acetylated alpha -tubulin (red) and gamma -tubulin (light blue). Scale bar, 10 μm. (e) Six hours after Ca2+ switch, mIMCD3 cells grown until confluence on filters were fixed with 4% PFA and stained for the apical marker Gp135 (red). Scale bar, 10 μm. (f) Percentage of normal spheroids of control and Traf3ip1-KD/ Map4-KD mIMCD3 cells expressing either GFP or IFT54-GFP fusions grown on Matrigel for 5 days (mean ± s.d., n≥100 spheroids from 3 independent experiments, ***P≤0,0001, *P<0.012, Bonferonni's multiple-comparison test). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Gdf2 deletion decreases tumor perfusion and maturation in the E0771 mammary cancer model. E0771 cells were injected in the 4th mammary gland of WT and Gdf2−/− mice and tumor vascularization was analyzed 9 days after tumor detection. a Representative images of the tumors stained for podocalyxin (red), lectin (green) and cell nuclei (blue, Hoechst). Scale bar 50 μm. b Vascular density quantified by podocalyxin positive area (% of tumor area) and (c) assessment of vessel diameter using Ferret’s theorem (WT n = 7, Gdf2−/−n = 13, 1 representative experiment out of 2). d Quantification of vessel perfusion by lectin staining (% area of lectin/podocalyxin) (WT n = 8, Gdf2−/− n = 7, 1 representative experiment out of 3). e Representative images of the tumors stained for podocalyxin (red), alpha -smooth muscle actin ( alpha -SMA) (green) and cell nuclei (blue, Hoechst). Scale bar 100 μm. f alpha -SMA staining quantification (% area of alpha -SMA/podocalyxin) (WT n = 8, Gdf2−/− n = 7, 1 representative experiment out of 3). b, c, d, f Data are the median ± interquartile range. Statistical analysis: Mann-Whitney test. *p ≤ 0.05 and **p ≤ 0.01 significantly different Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30165893), licensed under a CC-BY license. Not internally tested by R&D Systems.

Endothelial beta -catenin GOF does not affect the ECM of astrocytic endfeet and ECs within the subfornical organ (SFO).Striatal BBB-vessel showing a polarized distribution of Lama2 and Aqp4 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Striatal BBB-vessel showing a polarized distribution of ColIV (green) but no Meca32 (white) in ECs (D). Coronal overview SFO, rectangular inset demarcates area for higher magnification in F (E); white dashed lines show Meca32+, red dashed lines show Meca32 vessels (F). Dashed lines outline SFO vessels; scale bars show 2 µm (A), 50 µm (B), 10 µm (C), 2.5 µm (D), 50 µm (E), 10 µm (F). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30932814), licensed under a CC-BY license. Not internally tested by R&D Systems.

TRAF3IP1 mutations lead to epithelialization and polarity defects.(a) mIMCD3 cells grown until confluence on filters were subjected to Ca2+-free medium to disrupt the tight junctions. Six hours after Ca2+ addition, cells were analysed by immunofluorescence using the apical marker Gp135 (red) and beta -catenin (light blue) to stain the cell junctions. Scale bar, 10 μm. (b) Following Ca2+ switch, tight junction re-formation was assessed by measurement of trans-epithelial resistance (TER) at different time points (mean ± s.e.m. of n=5 independent experiments, two-way ANOVA; NS: not-significant, ***P<0.001 at 6 h). (c) Height of mIMCD3 cells grown on filters measured as the distance from the base to the top of the cells (GFP staining, not shown; mean ± s.d. of n≥20, from 3 independent experiments, ***P<0.001, Bonferonni's multiple-comparison test). (d) Expression of the apical marker Gp135 was analysed by Western blot with alpha -tubulin as a loading control. (e) mIMCD3 cells grown in matrigel 3D matrix to form spheroids were stained for ZO1 (tight junctions, red) and analysed by confocal microscopy. Arrows indicate ZO-1 at the apical junctions, while arrow heads point to mislocalized ZO-1. Equatorial sections of representative spheres are shown for each cell line. Scale bars, 10 μm. (f) Percentage of abnormal spheroids (no/small lumen filled with cells) (mean ± s.d., n=80 spheroids from 2 independent experiments, ***P≤0,001, **P<0.002, Bonferonni's multiple-comparison test). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Bmp10 conditional deletion has no impact on tumor growth, angiogenesis and lung metastasis in the E0771 mammary cancer model. a Schematic representation of the experimental protocol for Bmp10 specific deletion and E0771 cells implantation. Tamoxifen was injected in all 3-week-old mice; 3 weeks later, E0771 cells were injected and tumor growth was analyzed for 3 weeks. b Plasmatic levels of BMP10 in control (CTL, n = 15) and Bmp10 conditional KO (Bmp10-cKO, n = 15) mice assessed by ELISA at the end of the experiment. c Tumor growth was assessed by caliper measurement every 2 to 3 days after tumor detection (CTL n = 7, Bmp10-cKO n = 8, 1 representative experiment out of 3). d Representative images of the tumors stained for podocalyxin (red), lectin (green) and cell nuclei (blue, Hoechst). Scale bar 50 μm. e Vascular density quantified by podocalyxin surface area (% of tumor area) and (f) Quantification of vessel perfusion by lectin staining (% area of lectin/podocalyxin) (CTL n = 7, Bmp10-cKO n = 8, 1 representative experiment out of 3). g Total area, (h) number and (i) mean size of lung metastases per mice bearing metastases (CTL n = 10, Bmp10-cKO n = 9, 2 experiments). c Data are the mean ± SEM. Statistical analysis: Two-way matched ANOVA. b, e, f, g, h, i Data are the median ± interquartile range. Statistical analysis: Mann-Whitney test. ****p ≤ 0.001 significantly different Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30165893), licensed under a CC-BY license. Not internally tested by R&D Systems.

Endothelial beta -catenin GOF does not affect astrocytic endfoot polarization of alpha -dystroglycan ( alpha -Dag) and Kir4.1 within the subfornical organ (SFO).Striatal BBB-vessel showing a polarized distribution of alpha -Dag and Kir4.1 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Dashed lines outline SFO vessels. Scale bar show 2 µm (A), 50 µm (B), 10 µm (C). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30932814), licensed under a CC-BY license. Not internally tested by R&D Systems.

F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

Neuro-2A cells were stained with Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556, filled histogram) or isotype control antibody (Catalog # 4-001-A, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Improved survival&blood–brain barrier function post pneumococcal infection in mice by anti-HIF-1 alpha treatment using echinomycin. e Immunofluorescence staining for HIF-1 alpha , BBB permeability&junctional markers in the echinomycin&vehicle groups at the survival end point. Left panel shows that echinomycin treatment leads to a reduction in HIF-1 alpha -positive nuclei including in ECs co-stained for podocalyxin, a vascular marker which was unchanged by the treatment. Middle panel displays reduced vascular permeability to fibrinogen in the echinomycin group as indicated by stronger intravascular signal compared to the vehicle-treated mice. Increased expression of tight junction proteins—occludin (middle panel)&claudin-5 (right top panel)—in echinomycin-treated mice indicate improved BBB function. Tight junction-associated ZO-1, adherens junction marker VE-Cadherin,&endothelial cell adhesion molecule CD31 unchanged (middle, bottom right panel). There was also no difference in S. pneumoniae (Spn) staining (right top panel) between the two groups. Scale bar 10 μm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32529267), licensed under a CC-BY license. Not internally tested by R&D Systems.

miR-671-5p aggravates beta -catenin-induced podocyte injury while miR-671-5p inhibitor ameliorates it in vitro. (A–C) Representative Western blot (A) and graphic presentations of WT1 (B) and podocalyxin (C) were presented. MPC5 cells were transfected with miR-671-5p mimics (miR-671-5p) or/and beta -catenin expression plasmid (pDel-beta -cat) for 24 h *p < 0.05 versus pcDNA3 controls; †p < 0.05 versus pDel-beta -cat (n = 3). (D–H) Representative Western blot (D) and graphic presentations of podocalyxin (E), nephrin (F), ZO-1 (G) and WT1 (H) were presented. MPC5 cells were transfected with beta -catenin expression plasmid (pDel-beta -cat) or/and miR-671-5p inhibitor (AntimiR-671-5p) for 24 h *p < 0.05 (n = 3). (I) Representative micrographs show the expression of ZO-1 in different groups as indicated. MPC5 cells were transfected with beta -catenin expression plasmid (pDel-beta -cat) and miR-671-5p mimics (miR-671-5p)/miR-671-5p inhibitor (AntimiR-671-5p) for 24 h, respectively. Scale bar, 50 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35111054), licensed under a CC-BY license. Not internally tested by R&D Systems.

Overexpression of miR-671-5p impairs but knockdown of miR-671-5p protects podocyte integrity in vitro. Mouse podocytes (MPC5) were transfected with miR-671-5p mimics (miR-671-5p) or negative control (miR-Ctrl) for 24 h. (A) qRT-PCR analysis shows the relative levels of miR-671-5p after transfection. *p < 0.05 (n = 3). (B–E) Representative Western blot (B) and graphic presentations of WT1 (C), ZO-1 (D) and podocalyxin (E) were presented. *p < 0.05 (n = 3). (F) Representative micrographs show the expression and distribution of ZO-1 in podocytes after miR-671-5p overexpression. Scale bar, 50 µm. (G–K) Inhibition of miR-671-5p protects podocyte integrity. MPC5 cells were transfected with miR-671-5p inhibitor (AntimiR-671-5p) or control (AntimiR-Ctrl) or for 24 h. Representative Western blot (G) and graphic presentations of WT1 (H), nephrin (I), podocalyxin (J) and ZO-1 (K) were presented. *p < 0.05 (n = 3). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35111054), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of miR-671-5p reduces proteinuria and renal fibrotic lesions in ADR nephropathy. (A) Experimental design. Red Arrows indicate the time of ADR injection. Green arrows indicate the different time points of antagomir injections. (B) qRT-PCR analysis shows that miR-671-5p level was increased in ADR group compared with control, and injections of antimiR-671-5p decreased miR-671-5p level. *p < 0.05 versus normal controls; †p < 0.05 versus ADR (n = 5–6). (C) Inhibition of miR-671-5p reduces proteinuria in ADR nephropathy. Urinary albumin levels were assessed in mice at 2 weeks after ADR injection and expressed as mg/mg creatinine. *p < 0.05 versus normal controls; †p < 0.05 versus ADR (n = 5–6). (D–G) Representative Western blots (D) and graphic presentations of WT1 (E), podocalyxin (F) and nephrin (G) were presented. *p < 0.05 versus normal controls, †p < 0.05 versus ADR alone (n = 5–6). (H) Immunofluorescence staining shows that antimiR-671-5p preserved renal podocalyxin expression in ADR nephropathy. Arrow indicate positive staining. Scale bar, 20 µm. (I,J) Representative Western blots (I) and graphic presentations of fibronectin and alpha -SMA (J) were presented. *p < 0.05 versus normal controls, †p < 0.05 versus ADR alone (n = 5–6). (K) Representative micrographs show that antimiR-671-5p inhibited alpha -SMA expression (upper panel) and renal fibrotic lesions (bottom panel) in different groups as indicated. Scale bar, 50 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35111054), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1556U-100UG
Alexa Fluor 405	AF1556V-100UG
Alexa Fluor 488	AF1556G-100UG
Alexa Fluor 532	AF1556X-100UG
Alexa Fluor 594	AF1556T-100UG
Alexa Fluor 647	AF1556R-100UG
Alexa Fluor 700	AF1556N-100UG
Alexa Fluor 750	AF1556S-100UG
Alexa Fluor Plus 405	AF1556AFP405-100UG
Alexa Fluor Plus 488	AF1556AFP488-100UG
Alexa Fluor Plus 555	AF1556AFP555-100UG
Alexa Fluor Plus 594	AF1556AFP594-100UG
Alexa Fluor Plus 647	AF1556AFP647-100UG
Alexa Fluor Plus 680	AF1556AFP680-100UG
Allophycocyanin	AF1556A
Biotin	BAF1556
CoraFluor 1	AF1556CL1
DyLight 350	AF1556D
DyLight 405	AF1556E
DyLight 488	AF1556K
DyLight 550	AF1556L
DyLight 594	AF1556M
DyLight 650	AF1556W
DyLight 680	AF1556Y
DyLight 755	AF1556Z
FITC	AF1556F
HRP	AF1556H
Janelia Fluor 525	AF1556JF525
Janelia Fluor 549	AF1556I
Janelia Fluor 646	AF1556J
Janelia Fluor 669	AF1556JF669
PE	AF1556P
PerCP	AF1556C
mFluor Violet 450 SE	AF1556MFV450
mFluor Violet 500 SE	AF1556MFV500
mFluor Violet 610 SE	AF1556MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1556-SP

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(select up to 3 products from this page to compare)

AF1658

Podocalyxin Like Antibody [Unconjugated]

(42 Publications)

100 μg

AF1658

AB-108-C, open histograms), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody F0108). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

AB-108-C, open histograms), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody F0108). View our protocol for Staining Membrane-associated Proteins." alt="MDA-MB-231 (orange) or MG-63 (blue) human cell lines were stained with Goat Anti-Human Podocalyxin Polyclonal Antibody (Catalog # AF1658, filled histograms) or isotype control antibody AB-108-C, open histograms), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody F0108). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Podocalyxin was detected in immersion fixed paraffin-embedded sections of human kidney using 5 µg/mL Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for Podocalyxin at approximately 160 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Podocalyxin/PODXL knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Podocalyxin at approximately 160 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Genetic Strategies Validation. </b>Podocalyxin promotes lumenogenesis in epiblast spheroids.(a) Immunoblot for podocalyxin protein in two representative PODXL−/− hPSC mutant clones (m1 and m2), compared with CRISPR/Cas9 non-mutant wild-type clones (WT) or cells subjected to scrambled (scr) or podocalyxin (pod) siRNA knockdown. (b) Brightfield images of sandwiched parental ESCs were compared with two mutant or two WT CRISPR/Cas9 clones. (c) Cavitated spheroids as a percentage of total colonies. Data from pools of WT or mutant cell lines were averaged to determine group means (AVG, n≥9) and P values. (d) Podocalyxin and ZO-1 immunofluorescence in naive and primed hLR5 hPSCs or (e) mESCs and EpiSCs. (f) Confocal z-sections of undifferentiated hPSCs showing localization of ZO-1 and beta CAT in unmodified (WT) or PODXL−/− colonies. (g) Filamentous actin (f-actin) and occludin (OCLN) immunofluorescence in undifferentiated WT or PODXL−/− clones. (h) Averaged TEER measurements in WT or PODXL−/− monolayers (n≥3). Scale bars, 50 μm or (f,g) 20 μm. Error bars, s.e.m. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26493500), licensed under a CC-BY license. Not internally tested by R&D Systems.

Junctional complexes are disrupted in hPSC-derived PODXL−/− podocyte-like cells.(a) Confocal optical section of adult human kidney. Podocalyxin is expressed in podocytes and peritubular capillaries, but is absent from tubules (white dotted lines). Auto, autofluorescence. (b) Crumbs3 expression in hPSC-derived kidney organoids (confocal red channel) and human kidney tissue (far right panel, immunohistochemistry). (c) Confocal optical sections showing distributions of ZO-1 with podocalyxin or (d) beta CAT in hPSC-derived podocyte-like cell clusters. Arrowheads highlight tracks of junctional complexes between podocyte-like cells. (e) Confocal sections of wild-type or PODXL−/− podocyte-like cell clusters in tubular organoids. (f) Gap widths between adjacent podocyte-like cell nuclei in these cell lines (n≥100 gaps pooled from two experiments). Scale bars, 50 μm. Error bars, s.e.m. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26493500), licensed under a CC-BY license. Not internally tested by R&D Systems.

hPSCs form cavitated spheroids in 3D culture.(a) Schematic of spheroid-to-organoid culture protocol. (b) Phase contrast images of ESCs in sandwich (3D) or monolayer (2D) cultures. Consecutive days are shown, with d0 indicating the time point immediately before sandwiching. (c) Confocal optical sections showing PODXL, ZO-1 and beta CAT immunofluorescence through a representative spheroid with cavity. Vertical distance from top to bottom row is shown at left. (d) Representative brightfield images of hPSCs in 3D cultures that were dissociated (coloured frames) and passaged (matching coloured arrows). Dashed arrows represent serial passages in the 3D condition. Lower row shows cells plated into 2D cultures from dissociated spheroids from each passage. (e) Hematoxylin and eosin-stained sections of teratomas generated from hPSC serial 3D passages p3, p6 and p9 showing pigmented epithelium (ectoderm), cartilage (mesoderm) and glandular epithelium (endoderm). (f) Cell number (average of duplicate counts for each time point, or AVG of all five time points shown in the last column) in 2D and 3D cultures 72 h after plating. (g) Representative immunofluorescence images showing OCT4 and sex-determining region Y box-2 (SOX2) or tumor rejection antigen 1–60 (TRA-1-60) and NANOG localization in p3 and p7 serially sandwiched hPSCs. Scale bars, 100 μm. Error bars, s.e.m. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26493500), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reg family proteins stimulated PSCs activation contributing to fibrosis in chronic pancreatitis. (a) Immunofluorescence analysis of alpha-smooth muscle actin ( alpha SMA, an activated PSCs marker, red) in the pancreas sections of indicated genotypes at 2 weeks, and 4 weeks (n = 3–4 mice). Nuclei were counterstained by DAPI (blue). (b) The percentage of epithelial cells with alpha SMA positive signals. Results represent mean ± SEM (n = 3–4 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. **P < 0.01 (c) Cropped images of western blot gels of Amylase, alpha SMA, Desmin, Reg1, Reg2, Reg3b of the pancreas of indicated genotypes at 0.5 day after birth(P0.5), 1w and 4w, p44/42 MAPK (ERK 1/2) and Akt were used as the loading control (n = 3 mice). The samples derived from the same experiment and gels were processed in parallel. Images of the entire gels are presented in Supplementary Fig. 5–7. The right panel showed the respective densitometric quantification analysis of the relative intensity of alpha SMA. Densitometric quantification analysis of other proteins is presented in Supplementary Fig. 4.Results represent mean ± SEM (n = 3 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. ns: not significant; #P < 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37500741), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1658U-100UG
Alexa Fluor 405	AF1658V-100UG
Alexa Fluor 488	AF1658G-100UG
Alexa Fluor 532	AF1658X-100UG
Alexa Fluor 594	AF1658T-100UG
Alexa Fluor 647	AF1658R-100UG
Alexa Fluor 700	AF1658N-100UG
Alexa Fluor 750	AF1658S-100UG
Alexa Fluor Plus 405	AF1658AFP405-100UG
Alexa Fluor Plus 488	AF1658AFP488-100UG
Alexa Fluor Plus 555	AF1658AFP555-100UG
Alexa Fluor Plus 594	AF1658AFP594-100UG
Alexa Fluor Plus 647	AF1658AFP647-100UG
Alexa Fluor Plus 680	AF1658AFP680-100UG
Allophycocyanin	AF1658A
Biotin	BAF1658
CoraFluor 1	AF1658CL1
DyLight 350	AF1658D
DyLight 405	AF1658E
DyLight 488	AF1658K
DyLight 550	AF1658L
DyLight 594	AF1658M
DyLight 650	AF1658W
DyLight 680	AF1658Y
DyLight 755	AF1658Z
FITC	AF1658F
HRP	AF1658H
Janelia Fluor 525	AF1658JF525
Janelia Fluor 549	AF1658I
Janelia Fluor 646	AF1658J
Janelia Fluor 669	AF1658JF669
PE	AF1658P
PerCP	AF1658C
mFluor Violet 450 SE	AF1658MFV450
mFluor Violet 500 SE	AF1658MFV500
mFluor Violet 610 SE	AF1658MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1658-SP

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MAB1556

Podocalyxin Like Antibody (192703) [Unconjugated]

(2 Reviews)

(40 Publications)

100 μg

MAB1556

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Podocalyxin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Podocalyxin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Podocalyxin was detected in perfusion fixed frozen sections of mouse kidney tissue using Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) at 1.7 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Microglia in contact with retinal vessels. (a) Retinal vessels and microglia invasion 6 months after irradiation. Retina flat mounts stained with anti-podocalyxin (in red) to visualize luminal membrane of endothelial cells, anti-Iba1 (in green) to localize microglial cells or macrophages and Dapi (in blue) to stain nuclei. Scale bars represent 50 μm. (b) 3D-reconstruction of microglia invasion. Images from retina flat mounts stained with lectin (in green) to show vessel walls and anti-Iba1 (purple) to visualize microglial cells and macrophages. Scale bars represent 20 μm on the upper row, and 5 μm on the bottom row. Confocal microscopy images processed with IMARIS, Oxford Instrument, UK. Image collected and cropped by CiteAb from the following open publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-024-03151-2), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1556U-100UG
Alexa Fluor 405	FAB1556V-100UG
Alexa Fluor 488	FAB1556G-100UG
Alexa Fluor 532	FAB1556X
Alexa Fluor 594	FAB1556T-100UG
Alexa Fluor 647	FAB1556R-100UG
Alexa Fluor 700	FAB1556N-100UG
Alexa Fluor 750	FAB1556S-100UG
Alexa Fluor Plus 405	FAB1556AFP405-100UG
Alexa Fluor Plus 488	FAB1556AFP488-100UG
Alexa Fluor Plus 555	FAB1556AFP555-100UG
Alexa Fluor Plus 594	FAB1556AFP594-100UG
Alexa Fluor Plus 647	FAB1556AFP647-100UG
Alexa Fluor Plus 680	FAB1556AFP680-100UG
Allophycocyanin	FAB1556A
Allophycocyanin/Cy7	FAB1556APCCY7
Biotin	FAB1556B
CoraFluor 1	FAB1556CL1
DyLight 350	FAB1556D
DyLight 405	FAB1556E
DyLight 488	FAB1556K
DyLight 550	FAB1556L
DyLight 594	FAB1556M
DyLight 650	FAB1556W
DyLight 680	FAB1556Y
DyLight 755	FAB1556Z
FITC	FAB1556F
HRP	FAB1556H
Janelia Fluor 525	FAB1556JF525
Janelia Fluor 549	FAB1556I
Janelia Fluor 646	FAB1556J
Janelia Fluor 669	FAB1556JF669
PE/Atto594	FAB1556PEATT594
PE/Cy5.5	FAB1556PECY55
PE/Cy7	FAB1556PECY7
PerCP	FAB1556C
Phycoerythrin	FAB1556P
mFluor Violet 450 SE	FAB1556MFV450
mFluor Violet 500 SE	FAB1556MFV500
mFluor Violet 610 SE	FAB1556MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1556P
Carrier Free	FAB1556A
Carrier Free	MAB1556-SP
Carrier Free	FAB1556V-100UG
Carrier Free	FAB1556U-100UG
Carrier Free	FAB1556T-100UG
Carrier Free	FAB1556S-100UG
Carrier Free	FAB1556R-100UG
Carrier Free	FAB1556G-100UG
Carrier Free	FAB1556N-100UG

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MAB1658

Podocalyxin Like Antibody (222328) [Unconjugated]

(1 Review)

(25 Publications)

100 μg

MAB1658

MAB003, open histograms), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

MAB003, open histograms), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins." alt="MDA-MB-231 (orange) or MG-63 (blue) human cell lines were stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histograms) or isotype control antibody (MAB003, open histograms), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomerular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Podocalyxin was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomerular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Live-tracking of NPC rosette morphogenesis. (A–C) Live imaging of 1196a hiPSC expressing Lifeact-GFP (A) or PODXL-GFP (C) for 12 h after colonies were allowed to reattach for 2 h. (B) Confocal images of PODXL-GFP NPC rosettes stained with anti-PODXL. GFP localization mirrors endogenous PODXL localization. Note that, while a uniform prominent GFP signal is initially seen throughout the apical surface of the recently plated colony, at later time points, GFP-enriched regions are concentrated at the constricted centers of the rosettes (lumen). It is likely that, as the colony expands rapidly, the apical surface area of non-rosette cells dramatically increases, resulting in reduced GFP signal intensity per unit area. Scales as indicated. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33178701), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1658U-100UG
Alexa Fluor 405	FAB1658V-100UG
Alexa Fluor 488	FAB1658G-100UG
Alexa Fluor 532	FAB1658X
Alexa Fluor 594	FAB1658T-100UG
Alexa Fluor 647	FAB1658R-100UG
Alexa Fluor 700	FAB1658N-100UG
Alexa Fluor 750	FAB1658S-100UG
Alexa Fluor Plus 405	FAB1658AFP405-100UG
Alexa Fluor Plus 488	FAB1658AFP488-100UG
Alexa Fluor Plus 555	FAB1658AFP555-100UG
Alexa Fluor Plus 594	FAB1658AFP594-100UG
Alexa Fluor Plus 647	FAB1658AFP647-100UG
Alexa Fluor Plus 680	FAB1658AFP680-100UG
Allophycocyanin/Cy7	FAB1658APCCY7
Biotin	FAB1658B
CoraFluor 1	FAB1658CL1
DyLight 350	FAB1658D
DyLight 405	FAB1658E
DyLight 488	FAB1658K
DyLight 550	FAB1658L
DyLight 594	FAB1658M
DyLight 650	FAB1658W
DyLight 680	FAB1658Y
DyLight 755	FAB1658Z
FITC	FAB1658F
HRP	FAB1658H
Janelia Fluor 525	FAB1658JF525
Janelia Fluor 549	FAB1658I
Janelia Fluor 646	FAB1658J
Janelia Fluor 669	FAB1658JF669
PE/Atto594	FAB1658PEATT594
PE/Cy5.5	FAB1658PECY55
PE/Cy7	FAB1658PECY7
Phycoerythrin	FAB1658P
mFluor Violet 450 SE	FAB1658MFV450
mFluor Violet 500 SE	FAB1658MFV500
mFluor Violet 610 SE	FAB1658MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1658G-100UG
Carrier Free	FAB1658P
Carrier Free	MAB1658-SP
Carrier Free	FAB1658R-100UG
Carrier Free	FAB1658V-100UG
Carrier Free	FAB1658U-100UG
Carrier Free	FAB1658T-100UG
Carrier Free	FAB1658S-100UG
Carrier Free	FAB1658N-100UG

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NBP2-25219

Podocalyxin Like Antibody (3D3)

(9 Publications)

0.1 mg

NBP2-25219

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Like Antibody (3D3).

Flow Cytometry: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Surface staining of NCCIT cells using Podocalyxin antibody at 1 ug/10^6 cells. A FITC-conjugated anti mouse Ig secondary antibody was used along with this antibody .

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Formalin-paraffin angiosarcoma stained with Podocalyxin Ab (3D3).

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Like Antibody (3D3).

Reactivity:

Hu, Rt, Rb

Species Glossary

Reactivity: Hu, Rt, Rb

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

ABX-164A

Alt Catalog# ABX-164A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33108AF350
Alexa Fluor 405	NBP2-33108AF405
Alexa Fluor 488	NBP2-33108AF488
Alexa Fluor 532	NBP2-33108AF532
Alexa Fluor 594	NBP2-33108AF594
Alexa Fluor 647	NBP2-33108AF647
Alexa Fluor 700	NBP2-33108AF700
Alexa Fluor 750	NBP2-33108AF750
Alexa Fluor Plus 405	NBP2-33108AFP405
Alexa Fluor Plus 488	NBP2-33108AFP488
Alexa Fluor Plus 555	NBP2-33108AFP555
Alexa Fluor Plus 594	NBP2-33108AFP594
Alexa Fluor Plus 647	NBP2-33108AFP647
Alexa Fluor Plus 680	NBP2-33108AFP680
Allophycocyanin	NBP2-33108APC
Allophycocyanin/Cy7	NBP2-33108APCCY7
Biotin	NBP2-33108B
CoraFluor 1	NBP2-33108CL1
DyLight 350	NBP2-33108UV
DyLight 405	NBP2-33108V
DyLight 488	NBP2-33108G
DyLight 550	NBP2-33108R
DyLight 594	NBP2-33108DL594
DyLight 650	NBP2-33108C
DyLight 680	NBP2-33108FR
DyLight 755	NBP2-33108IR
FITC	NBP2-33108F
HRP	NBP2-33108H
Janelia Fluor 525	NBP2-33108JF525
Janelia Fluor 549	NBP2-33108JF549
Janelia Fluor 646	NBP2-33108JF646
Janelia Fluor 669	NBP2-33108JF669
PE	NBP2-33108PE
PE/Atto594	NBP2-33108PEATT594
PE/Cy5.5	NBP2-33108PECY55
PE/Cy7	NBP2-33108PECY7
PerCP	NBP2-33108PCP
mFluor Violet 450 SE	NBP2-33108MFV450
mFluor Violet 500 SE	NBP2-33108MFV500
mFluor Violet 610 SE	NBP2-33108MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33108
IHC-Prediluted	NBP2-45167

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NB110-41503

Podocalyxin Like Antibody - BSA Free

(1 Review)

(2 Publications)

0.1 ml

NB110-41503

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-41503AF350
Alexa Fluor 405	NB110-41503AF405
Alexa Fluor 488	NB110-41503AF488
Alexa Fluor 532	NB110-41503AF532
Alexa Fluor 594	NB110-41503AF594
Alexa Fluor 647	NB110-41503AF647
Alexa Fluor 700	NB110-41503AF700
Alexa Fluor 750	NB110-41503AF750
Alexa Fluor Plus 405	NB110-41503AFP405
Alexa Fluor Plus 488	NB110-41503AFP488
Alexa Fluor Plus 555	NB110-41503AFP555
Alexa Fluor Plus 594	NB110-41503AFP594
Alexa Fluor Plus 647	NB110-41503AFP647
Alexa Fluor Plus 680	NB110-41503AFP680
Allophycocyanin	NB110-41503APC
Allophycocyanin/Cy7	NB110-41503APCCY7
Biotin	NB110-41503B
CoraFluor 1	NB110-41503CL1
DyLight 350	NB110-41503UV
DyLight 405	NB110-41503V
DyLight 488	NB110-41503G
DyLight 550	NB110-41503R
DyLight 594	NB110-41503DL594
DyLight 650	NB110-41503C
DyLight 680	NB110-41503FR
DyLight 755	NB110-41503IR
FITC	NB110-41503F
HRP	NB110-41503H
Janelia Fluor 525	NB110-41503JF525
Janelia Fluor 549	NB110-41503JF549
Janelia Fluor 646	NB110-41503JF646
Janelia Fluor 669	NB110-41503JF669
PE	NB110-41503PE
PerCP	NB110-41503PCP
mFluor Violet 450 SE	NB110-41503MFV450
mFluor Violet 500 SE	NB110-41503MFV500
mFluor Violet 610 SE	NB110-41503MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-41503SS

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NBP1-83348

Podocalyxin Like Antibody - BSA Free

(8 Publications)

0.1 ml

NBP1-83348

Western Blot: Podocalyxin Like Antibody [NBP1-83348] - Analysis in human kidney tissue.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Analysis in human kidney and liver tissues. Corresponding Podocalyxin Like RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.

Staining of human rectum shows strong membranous positivity in endothelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-83348-25ul

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NBP2-45362

Podocalyxin Like Antibody (OTI2C6)

0.1 ml

NBP2-45362

Immunohistochemistry: Podocalyxin Antibody (2C6) [NBP2-45362] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Podocalyxin Antibody (2C6) [NBP2-45362] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Podocalyxin Antibody (2C6) [NBP2-45362] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Podocalyxin Antibody (2C6) [NBP2-45362] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Podocalyxin Antibody (2C6) [NBP2-45362] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Podocalyxin Antibody (2C6) [NBP2-45362] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Podocalyxin Antibody (2C6) [NBP2-45362] - Analysis of Adenocarcinoma of Human endometrium tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Podocalyxin Antibody (2C6) [NBP2-45362] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Podocalyxin Antibody (2C6) [NBP2-45362] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73511AF350
Alexa Fluor 405	NBP2-73511AF405
Alexa Fluor 488	NBP2-73511AF488
Alexa Fluor 532	NBP2-73511AF532
Alexa Fluor 594	NBP2-73511AF594
Alexa Fluor 647	NBP2-73511AF647
Alexa Fluor 700	NBP2-73511AF700
Alexa Fluor 750	NBP2-73511AF750
Alexa Fluor Plus 405	NBP2-73511AFP405
Alexa Fluor Plus 488	NBP2-73511AFP488
Alexa Fluor Plus 555	NBP2-73511AFP555
Alexa Fluor Plus 594	NBP2-73511AFP594
Alexa Fluor Plus 647	NBP2-73511AFP647
Alexa Fluor Plus 680	NBP2-73511AFP680
Allophycocyanin	NBP2-73511APC
Biotin	NBP2-73511B
CoraFluor 1	NBP2-73511CL1
DyLight 350	NBP2-73511UV
DyLight 405	NBP2-73511V
DyLight 488	NBP2-73511G
DyLight 550	NBP2-73511R
DyLight 594	NBP2-73511DL594
DyLight 650	NBP2-73511C
DyLight 680	NBP2-73511FR
DyLight 755	NBP2-73511IR
FITC	NBP2-73511F
HRP	NBP2-73511H
Janelia Fluor 525	NBP2-73511JF525
Janelia Fluor 549	NBP2-73511JF549
Janelia Fluor 646	NBP2-73511JF646
Janelia Fluor 669	NBP2-73511JF669
PE	NBP2-73511PE
PerCP	NBP2-73511PCP
mFluor Violet 450 SE	NBP2-73511MFV450
mFluor Violet 500 SE	NBP2-73511MFV500
mFluor Violet 610 SE	NBP2-73511MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73511

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NBP2-45169

Podocalyxin Like Antibody (2A4)

(1 Publication)

0.1 mg

NBP2-45169

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (2A4) [NBP2-45169] - Human Angiosarcoma stained with Podocalyxin Monoclonal Antibody (2A4).

Reactivity:

Hu, Rt, Rb

Species Glossary

Reactivity: Hu, Rt, Rb

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-47750-0.1mg
IHC-Prediluted	NBP2-48344

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NBP2-52903

Podocalyxin Like Antibody (CL0284) - BSA Free

0.1 ml

NBP2-52903

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0284) [NBP2-52903] - Staining in human kidney and liver tissues. Corresponding PODXL RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0284) [NBP2-52903] - Staining of human kidney shows strong membranous positivity in cells in glomeruli, as well as endothelial cells.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0284) [NBP2-52903] - Staining of human fallopian tube shows strong positivity in apical membranes in glandular cells.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0284) [NBP2-52903] - Staining of human rectum shows moderate membranous positivity in endothelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43980AF350
Alexa Fluor 405	NBP3-43980AF405
Alexa Fluor 488	NBP3-43980AF488
Alexa Fluor 532	NBP3-43980AF532
Alexa Fluor 594	NBP3-43980AF594
Alexa Fluor 647	NBP3-43980AF647
Alexa Fluor 700	NBP3-43980AF700
Alexa Fluor 750	NBP3-43980AF750
Alexa Fluor Plus 405	NBP3-43980AFP405
Alexa Fluor Plus 488	NBP3-43980AFP488
Alexa Fluor Plus 555	NBP3-43980AFP555
Alexa Fluor Plus 594	NBP3-43980AFP594
Alexa Fluor Plus 647	NBP3-43980AFP647
Alexa Fluor Plus 680	NBP3-43980AFP680
Allophycocyanin	NBP3-43980APC
Biotin	NBP3-43980B
CoraFluor 1	NBP3-43980CL1
DyLight 350	NBP3-43980UV
DyLight 405	NBP3-43980V
DyLight 488	NBP3-43980G
DyLight 550	NBP3-43980R
DyLight 594	NBP3-43980DL594
DyLight 650	NBP3-43980C
DyLight 680	NBP3-43980FR
DyLight 755	NBP3-43980IR
FITC	NBP3-43980F
HRP	NBP3-43980H
Janelia Fluor 525	NBP3-43980JF525
Janelia Fluor 549	NBP3-43980JF549
Janelia Fluor 646	NBP3-43980JF646
Janelia Fluor 669	NBP3-43980JF669
PE	NBP3-43980PE
PerCP	NBP3-43980PCP
mFluor Violet 450 SE	NBP3-43980MFV450
mFluor Violet 500 SE	NBP3-43980MFV500
mFluor Violet 610 SE	NBP3-43980MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43980
BSA Free	NBP2-52903-25ul

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NBP2-52912

Podocalyxin Like Antibody (CL0308) - BSA Free

0.1 ml

NBP2-52912

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Staining of human fallopian tube shows moderate positivity in apical membranes of glandular cells.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Staining of human rectum shows weak to moderate membranous positivity in endothelial cells.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0308) [NBP2-52912] - Staining of human liver shows no positivity in hepatocytes as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43981AF350
Alexa Fluor 405	NBP3-43981AF405
Alexa Fluor 488	NBP3-43981AF488
Alexa Fluor 532	NBP3-43981AF532
Alexa Fluor 594	NBP3-43981AF594
Alexa Fluor 647	NBP3-43981AF647
Alexa Fluor 700	NBP3-43981AF700
Alexa Fluor 750	NBP3-43981AF750
Alexa Fluor Plus 405	NBP3-43981AFP405
Alexa Fluor Plus 488	NBP3-43981AFP488
Alexa Fluor Plus 555	NBP3-43981AFP555
Alexa Fluor Plus 594	NBP3-43981AFP594
Alexa Fluor Plus 647	NBP3-43981AFP647
Alexa Fluor Plus 680	NBP3-43981AFP680
Allophycocyanin	NBP3-43981APC
Biotin	NBP3-43981B
CoraFluor 1	NBP3-43981CL1
DyLight 350	NBP3-43981UV
DyLight 405	NBP3-43981V
DyLight 488	NBP3-43981G
DyLight 550	NBP3-43981R
DyLight 594	NBP3-43981DL594
DyLight 650	NBP3-43981C
DyLight 680	NBP3-43981FR
DyLight 755	NBP3-43981IR
FITC	NBP3-43981F
HRP	NBP3-43981H
Janelia Fluor 525	NBP3-43981JF525
Janelia Fluor 549	NBP3-43981JF549
Janelia Fluor 646	NBP3-43981JF646
Janelia Fluor 669	NBP3-43981JF669
PE	NBP3-43981PE
PerCP	NBP3-43981PCP
mFluor Violet 450 SE	NBP3-43981MFV450
mFluor Violet 500 SE	NBP3-43981MFV500
mFluor Violet 610 SE	NBP3-43981MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43981
BSA Free	NBP2-52912-25ul

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NBP2-52904

Podocalyxin Like Antibody (CL0285) - BSA Free

0.1 ml

NBP2-52904

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0285) [NBP2-52904] - Staining of human kidney shows strong membranous positivity in cells in glomeruli, as well as endothelial cells.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0285) [NBP2-52904] - Staining of human fallopian tube shows strong positivity in apical membranes in glandular cells.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0285) [NBP2-52904] - Staining of human rectum shows moderate membranous positivity in endothelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43854AF350
Alexa Fluor 405	NBP3-43854AF405
Alexa Fluor 488	NBP3-43854AF488
Alexa Fluor 532	NBP3-43854AF532
Alexa Fluor 594	NBP3-43854AF594
Alexa Fluor 647	NBP3-43854AF647
Alexa Fluor 700	NBP3-43854AF700
Alexa Fluor 750	NBP3-43854AF750
Alexa Fluor Plus 405	NBP3-43854AFP405
Alexa Fluor Plus 488	NBP3-43854AFP488
Alexa Fluor Plus 555	NBP3-43854AFP555
Alexa Fluor Plus 594	NBP3-43854AFP594
Alexa Fluor Plus 647	NBP3-43854AFP647
Alexa Fluor Plus 680	NBP3-43854AFP680
Allophycocyanin	NBP3-43854APC
Biotin	NBP3-43854B
CoraFluor 1	NBP3-43854CL1
DyLight 350	NBP3-43854UV
DyLight 405	NBP3-43854V
DyLight 488	NBP3-43854G
DyLight 550	NBP3-43854R
DyLight 594	NBP3-43854DL594
DyLight 650	NBP3-43854C
DyLight 680	NBP3-43854FR
DyLight 755	NBP3-43854IR
FITC	NBP3-43854F
HRP	NBP3-43854H
Janelia Fluor 525	NBP3-43854JF525
Janelia Fluor 549	NBP3-43854JF549
Janelia Fluor 646	NBP3-43854JF646
Janelia Fluor 669	NBP3-43854JF669
PE	NBP3-43854PE
PerCP	NBP3-43854PCP
mFluor Violet 450 SE	NBP3-43854MFV450
mFluor Violet 500 SE	NBP3-43854MFV500
mFluor Violet 610 SE	NBP3-43854MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43854
BSA Free	NBP2-52904-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Podocalyxin Like Antibodies

Podocalyxin Like Antibody [Unconjugated]

Podocalyxin Like Antibody [Unconjugated]

Podocalyxin Like Antibody (192703) [Unconjugated]

Podocalyxin Like Antibody (222328) [Unconjugated]

Podocalyxin Like Antibody (3D3)

Podocalyxin Like Antibody - BSA Free

Podocalyxin Like Antibody - BSA Free

Podocalyxin Like Antibody (OTI2C6)

Podocalyxin Like Antibody (2A4)

Podocalyxin Like Antibody (CL0284) - BSA Free

Podocalyxin Like Antibody (CL0308) - BSA Free

Podocalyxin Like Antibody (CL0285) - BSA Free