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HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">
AB-108-C, open histograms), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody F0108). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank">
HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF017). A specific band was detected for Podocalyxin at approximately 160 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.
![Western Blot Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Podocalyxin_AF1556_Western_Blot_12581.jpg)
![N/A Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af1658_human-podocalyxin-affinity-purified-polyclonal-ab-2682025114751.jpg)
![Immunocytochemistry Podocalyxin Like Antibody (192703) [Unconjugated]](http://images.novusbio.com/images/antibody/Podocalyxin_MAB1556_Immunocytochemistry_6569.jpg)
![Bioactivity Podocalyxin Like [Unconjugated]](http://images.novusbio.com/fullsize/protein/Podocalyxin_1658PD_1053.jpg)
![Bioactivity Podocalyxin Like [Unconjugated]](http://images.novusbio.com/fullsize/protein/Podocalyxin_1556PD_1561.jpg)
![Immunohistochemistry Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Podocalyxin_AF1556_Immunohistochemistry_11445.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-412024104815.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-4120241048246.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-41202410484320.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-41202410484311.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-41202410485429.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-41202410484394.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-41202410482425.jpg)
![Western Blot Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-4120241048113.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-41202410481116.jpg)
![Flow Cytometry Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-flow-cytometry-342024203321..jpg)
![Immunohistochemistry Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-22820241715324.jpg)
![Immunohistochemistry Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-812025557275.jpg)
![Western Blot Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-western-blot-121220258222419.jpg)
![Western Blot Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-western-blot-12122025825316.jpg)
![Western Blot Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-western-blot-121220258452119.jpg)
![Western Blot Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-western-blot-121220258452116.jpg)
![Western Blot Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Podocalyxin_AF1658_Western_Blot_12580.jpg)
![Immunohistochemistry Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Podocalyxin_AF1658_Immunohistochemistry_6712.jpg)
![Knockout Validated Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Podocalyxin_AF1658_Knockout_Validated_21955.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1658_human-podocalyxin-affinity-purified-polyclonal-ab-41202410482423.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1658_human-podocalyxin-affinity-purified-polyclonal-ab-41202410484369.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1658_human-podocalyxin-affinity-purified-polyclonal-ab-41202410481165.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1658_human-podocalyxin-affinity-purified-polyclonal-ab-4120241049425.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1658_human-podocalyxin-affinity-purified-polyclonal-ab-4120241049446.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1658_human-podocalyxin-affinity-purified-polyclonal-ab-4120241048543.jpg)
![Western Blot Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1658_human-podocalyxin-affinity-purified-polyclonal-ab-western-blot-12122025857415.jpg)
![Western Blot Podocalyxin Like Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1658_human-podocalyxin-affinity-purified-polyclonal-ab-western-blot-121220259163810.jpg)
![Immunohistochemistry Podocalyxin Like Antibody (192703) [Unconjugated]](http://images.novusbio.com/images/antibody/Podocalyxin_MAB1556_Immunohistochemistry_24137.jpg)
![Immunohistochemistry Podocalyxin Like Antibody (192703) [Unconjugated]](http://images.novusbio.com/fullsize/mab1556_mouse-podocalyxin-mab-clone-192703-immunohistochemistry-1212202575047.jpg)
