PINK1 Antibody

Knockdown Validated: PINK1 Antibody [BC100-494] - HEK293 cells were co-transfected with PINK1 siRNA (#1 or #2) or scrambled siRNA (scrambled) and untagged wild-type (WT) or Ser65Ala (S65A) mutant Parkin as indicated using TransFectin reagent (Bio-Rad). 48 hrs post-transfection, cells were treated with/without 10 uM CCCP for 3 h. 0.25 mg of 1% Triton whole-cell lysate were subjected to immunoprecipitation with GST-Parkin antibody (S966C) covalently coupled to protein G Sepharose and then immunoblotted with anti-phospho-Ser65 antibody in the presence of dephosphorylated peptide. 5% of the IP was immunoblotted with total anti-Parkin antibody. 0.25 mg of whole-cell lysates were immunoprecipitated with anti-PINK1 antibody (S085D) and immunoblotted with anti-PINK1 antibody. Representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (//rsob.royalsocietypublishing.org/cgi/doi/10.1098/rsob.120080) licensed under a CC-BY licence.

Western Blot: PINK1 Antibody [BC100-494] - Pathogenic mutants of Parkin are subjected to Ser65 phosphorylation. Phos-tag Western blotting for Parkin and Western blotting for PINK1 were performed using Parkin WT and a series of pathogenic mutants. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep01002) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: PINK1 Antibody [BC100-494] - Immunocytochemistry of PINK1 antibody (BC100-494 Lot G). HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with BC100-494 at 20 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Fluor488 anti-rabbit secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-mouse DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP.

Western Blot: PINK1 Antibody [BC100-494] - Alteration of mitochondria and PD associated proteins in SH-SY5Y cells with telomere removal by CRISPR-Cas9. Representative Western blot of PGC-1alpha, NRF1, and PINK1 in SH-SY5Y cells transfected with either gTel or gCont (72 h). beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/18/10/2093), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: PINK1 Antibody [BC100-494] - Rabbit heart tissue. Image from verified customer review.

Western Blot: PINK1 Antibody [BC100-494] - Western blot image of PINK1 antibody (BC100-494) in multiple cells lines. Human HeLa (lane 1), Mouse NIH-3T3 (lane 2), L929 (lane 3) and Rat PC12 (lane 4) whole cell protein were separated by SDS-PAGE on a 7.5% polyacrylamide gel. Protein was transferred to PVDF membrane and probed with 2 ug/ml BC100-494 in 1% BSA and detected with an HRP-conjugated anti-rabbit secondary antibody using chemiluminescence. Observed molecular weight ~55 kDa (arrowhead).

Western Blot: PINK1 Antibody [BC100-494] - Analysis of PINK1 in mouse liver and hypatocytes using PINK1 antibody. Image from verified customer review. Observed molecular weight ~55 kDa.

Knockout Validated: PINK1 Antibody [BC100-494] - Characterisation of mitochondrial content and function in PINK1-/- platelets. Cropped immunoblots showing PINK1 expression in 10 uM CCCP (6 hours) treated WT, but not KO platelets by IP (upper panel) with loading control of IP inputs by blotting for PINK1 (lower panel). Uncropped blots for PINK1 are provided in Supplementary Fig. S1b. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32716-4), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Dr, Rb
• Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PAGE, PEP-ELISA, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PINK1 Antibody Summary

• Immunogen: PINK1 antibody was developed using a synthetic peptide made to the human PINK1 protein sequence (between residues 175-250). [Swiss-Prot Q9BXM7]
• Localization: Localizes mostly in mitochondrion and the 2 proteolytic processed fragments (Topological domain 111-581) of 55 kDa and 48 kDa localize mainly in cytosol.
• Specificity: Human PINK1 Antibody will be reactive to isoform 2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PINK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Peptide ELISA 1:100-1:2000
  • SDS-Page
  • Knockdown Validated
  • Knockout Validated
• Application Notes: NOTE: It's recomended to use 1-5% w/v BSA in TBS with 0.1% Tween-20 for all incubations in WB. This PINK1 antibody can be used for ICC, Peptide ELISA and Western blot, where specific bands are seen at 48, 55 and 63 kDa. In WB, this antibody has been used in valinomycin and CCCP treated HeLa whole cell lysate. Use in IP reported in scientific literature (PMID 22078885) Use in paraffin-sections reported in scientific literature (PMID 25083992). Use in Immunoblotting reported in multiple pieces of scientific literature. Use in SDS-Page reported in scientific literature (PMID: 27846363). Use in Knockout Validated reported in scientific literature (PMID: 31066324). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31908016).
• Theoretical MW: 62.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  PINK1 Protein (BC100-494PEP)

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 30237395).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PINK1 Antibody

• Anti-PINK1 Antibody
• BRPK Antibody
• BRPK
• EC 2.7.11.1
• FLJ27236
• Mitochondrial Antibody
• PARK6
• Parkinson disease (autosomal recessive) 6
• PINK1 Antibody
• PINK1 mouse
• PINK1 polyclonal
• PINK1
• protein kinase BRPK
• PTEN Induced Kinase 1
• PTEN induced putative kinase 1
• PTEN-induced putative kinase protein 1
• serine/threonine-protein kinase PINK1, mitochondrial

Background

Phosphatase and Tensin Homolog (PTEN) is a tumor suppressor which acts as an antagonist to phosphatidylinositol 3-kinase (PI3K) signaling. PTEN exerts enzymatic activity as a phosphatidylinositol-3,4,5-trisphosphate (PIP3) phosphatase, opposing PI3K activity by reducing availability of PIP3 to proliferating cells. Loss of PTEN function leads to elevated PIP3 and increased activation of PI3K/AKT signaling in many types of cancer.

PINK1 (PTEN induced putative kinase 1) protein contains a N-terminal mitochondrial targeting sequence, putative transmembrane helix, linker region, serine (Ser65)/threonine (Thr257) kinase domain and C-terminal segment. PINK1 is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function. In damaged (depolarized) mitochondria, PINK1 becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface.

When PINK1 is imported into the cell, mitochondrial processing peptidase, presenilin-associated rhomboid-like protease and AFG3L2 cleave PINK1 and tag it for the ubiquitin-proteasome pathway, keeping low PINK1 protein expression at basal conditions (1,2). Accumulation of PINK1 in mitochondria indicate damage. PINK1 maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) (3). PINK1 has a theoretical molecular weight of 63 kDa and undergoes proteolytic processing to generate at least two cleaved forms (55 kDa and 42 kDa).

Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by 1) PINK-mediated phosphorylation of PARK2 at serine 65, and 2) PARK2 interaction with phosphorylated ubiquitin (also phosphorylated by PINK1 on serine 65) (4,5). There is a strong interplay between Parkin and PINK1, where loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by Parkin (2,4,5). Mutations in either Parkin or PINK1 alter mitochondrial turnover, resulting in the accumulation of defective mitochondria and, ultimately, neurodegeneration in Parkinson's disease. Mutations in the PINK1 gene located within the PARK6 locus on chromosome 1p35-p36 have been identified in patients with early-onset Parkinson's disease (6).

References

1.Rasool, S., Soya, N., Truong, L., Croteau, N., Lukacs, G. L., & Trempe, J. F. (2018). PINK1 autophosphorylation is required for ubiquitin recognition. EMBO Rep, 19(4). doi:10.15252/embr.201744981

2.Shiba-Fukushima, K., Arano, T., Matsumoto, G., Inoshita, T., Yoshida, S., Ishihama, Y.,... Imai, Y. (2014). Phosphorylation of mitochondrial polyubiquitin by PINK1 promotes Parkin mitochondrial tethering. PLoS Genet, 10(12), e1004861. doi:10.1371/journal.pgen.1004861

3.Vives-Bauza, C., Zhou, C., Huang, Y., Cui, M., de Vries, R. L., Kim, J.,... Przedborski, S. (2010). PINK1-dependent recruitment of Parkin to mitochondria in mitophagy. Proc Natl Acad Sci U S A, 107(1), 378-383. doi:10.1073/pnas.0911187107

4.McWilliams, T. G., Barini, E., Pohjolan-Pirhonen, R., Brooks, S. P., Singh, F., Burel, S.,... Muqit, M. M. K. (2018). Phosphorylation of Parkin at serine 65 is essential for its activation in vivo. Open Biol, 8(11). doi:10.1098/rsob.180108

5.Exner, N., Treske, B., Paquet, D., Holmstrom, K., Schiesling, C., Gispert, S.,... Haass, C. (2007). Loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by parkin. J Neurosci, 27(45), 12413-12418. doi:10.1523/jneurosci.0719-07.2007

6.Valente, E. M., Bentivoglio, A. R., Dixon, P. H., Ferraris, A., Ialongo, T., Frontali, M.,... Wood, N. W. (2001). Localization of a novel locus for autosomal recessive early-onset parkinsonism, PARK6, on human chromosome 1p35-p36. Am J Hum Genet, 68(4), 895-900. doi:10.1086/319522

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PINK1 Antibody (BC100-494)(156)

Publications using BC100-494

• Wang X, Feng L, Xin M et al. Mechanisms underlying astrocytic connexin-43 autophagy degradation during cerebral ischemia injury and the effect on neuroinflammation and cell apoptosis Biomed. Pharmacother. Apr 28 2020 [PMID: 32361163] (WB, Mouse)
• Nikolaou PE, Efentakis P, Qourah FA et al. Chronic Empaglifozin treatment reduces myocardial infarct size in non-diabetic mice through STAT-3 mediated protection on microvascular endothelial cells and reduction of oxidative stress Antioxid. Redox Signal. Apr 16 2020 [PMID: 32295413]
• Sridevi Gurubaran I, Viiri J, Koskela A et Al. Mitophagy in the Retinal Pigment Epithelium of Dry Age-Related Macular Degeneration Investigated in the NFE2L2/PGC-1 alpha -/- Mouse Model Int J Mol Sci Mar 13 2020 [PMID: 32183173] (Mouse)
• Bhupana Jn, Huang Bt, Liou Gg et Al. Gas7 knockout affects PINK1 expression and mitochondrial dynamics in mouse cortical neurons FASEB Bioadv Mar 1 2020 [PMID: 32161906] (Mouse)
• Franco-Iborra S, Plaza-Zabala A, Montpeyo M et al. Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease Autophagy Feb 24 2020 [PMID: 32093570]
• Sarkar A, Stellrecht CM, Vangapandu HV et al. Ataxia telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma Haematologica Feb 6 2020 [PMID: 32029507]
• Igarashi R, Yamashita SI, Yamashita T et al. Gemcitabine induces Parkin-independent mitophagy through mitochondrial-resident E3 ligase MUL1-mediated stabilization of PINK1 Sci Rep Jan 30 2020 [PMID: 32001742] (WB, ICC/IF, Human)
• Park SY, Koh HC FUNDC1 regulates receptor-mediated mitophagy independently of the PINK1/Parkin-dependent pathway in rotenone-treated SH-SY5Y cells Food Chem. Toxicol. Jan 27 2020 [PMID: 32001317] (WB, Human)
• Furlong RM, O'Keeffe GW, O'Neill C, Sullivan AM Alterations in alpha-synuclein and PINK1 expression reduce neurite length and induce mitochondrial fission and Golgi fragmentation in midbrain neurons Neurosci. Lett. Jan 21 2020 [PMID: 31978495] (ICC/IF, Rat)
• Ren YZ, Zhang BZ, Zhao XJ, Zhang ZY Resolvin D1 ameliorates cognitive impairment following traumatic brain injury via protecting astrocytic mitochondria J. Neurochem. Jan 17 2020 [PMID: 31951012] (WB, Mouse)
• Gillmore T Mitochondrial Dynamics in Mesenchymal Cells in Placental Development and Preeclampsia Thesis
• Riis S, Murray JB, O'Connor R IGF-1 Signalling Regulates Mitochondria Dynamics and Turnover through a Conserved GSK-3 beta-Nrf2-BNIP3 Pathway Cells Jan 8 2020 [PMID: 31936236] (Human, Mouse)
• Huang Y, Gu C, Wang Q, et al. The protective effort of GPCR kinase 2 interacting protein 1 in neurons via promoting Beclin1 Parkin induced mitophagy at the early stage of spinal cord ischemia reperfusion injury FASEB j. Dec 27 2019 [PMID: 31908016] (WB, ICC/IF, IHC-F, Mouse)
• Qu F, Wang P, Zhang K et al. Manipulation of Mitophagy by "All-in-One" nanosensitizer augments sonodynamic glioma therapy Autophagy Nov 9 2019 [PMID: 31674265] (ICC/IF, Mouse)
• Niu K, Fang H, Chen Z et al. USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy Autophagy Aug 26 2019 [PMID: 31432739] (WB, KD, Human)
• Miyai T, Vasanth S, Melangath G et al. Activation of PINK1-Parkin-Mediated Mitophagy Degrades Mitochondrial Quality Control Proteins in Fuchs Endothelial Corneal Dystrophy Am. J. Pathol. Jul 27 2019 [PMID: 31361992]
• Tomita K, Takashi Y, Ouchi Y et al. Lipid peroxidation increases hydrogen peroxide permeability leading to cell death in cancer cell lines that lack mtDNA Cancer Sci. Sep 1 2019 [PMID: 31314163] (ICC/IF, Human)
• Guardia-Laguarta C, Liu Y, Lauritzen KH et al. PINK1 Content in Mitochondria is Regulated by ER-Associated Degradation J. Neurosci. Jul 12 2019 [PMID: 31300519]
• Martin-Maestro P, Gargini R, Garcia E et al. Mitophagy Failure in APP and Tau Overexpression Model of Alzheimer's Disease J. Alzheimers Dis. Jun 24 2019 [PMID: 31256128]
• Lin Q, Li S, Jiang N et al. PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation Redox Biol. 2019 Jun 11 [PMID: 31229841] (ICC/IF, WB, Mouse)
• Martin-Maestro P, Sproul A, Martinez H et al. Autophagy Induction by Bexarotene Promotes Mitophagy in Presenilin 1 Familial Alzheimer's Disease iPSC-Derived Neural Stem Cells Mol. Neurobiol. Jun 16 2019 [PMID: 31203573]
• Yan C, Gong L, Chen L et al. PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis Autophagy. Jun 16 2019 [PMID: 31177901] (WB, IP, Human)
• Ganzleben I, He GW, Gunther C et al. PGAM5 is a key driver of mitochondrial dysfunction in experimental lung fibrosis Cell. Mol. Life Sci. Jun 5 2019 [PMID: 31168659]
• Warnez-Soulie J, Macia M, Lac S et al. Tumor protein 53-induced nuclear protein 1 deficiency alters mouse gastrocnemius muscle function and bioenergetics in vivo Physiol Rep May 1 2019 [PMID: 31124296] (WB, Mouse)
• Cheng G, Zhang Q, Pan J et al. Targeting lonidamine to mitochondria mitigates lung tumorigenesis and brain metastasis Nat Commun May 17 2019 [PMID: 31101821] (WB, Human)
• Livingston MJ, Wang J, Zhou J et al. Clearance of damaged mitochondria via mitophagy is important to the protective effect of ischemic preconditioning in kidneys Autophagy May 22 2019 [PMID: 31066324] (ICC/IF, KO, WB, Human)
• Audesse, AJ;Dhakal, S;Hassell, LA;Gardell, Z;Nemtsova, Y;Webb, AE; FOXO3 directly regulates an autophagy network to functionally regulate proteostasis in adult neural stem cells PLoS Genet. Apr 1 2019 [PMID: 30973875] (WB, Mouse)
• Eid N, Ito Y, Horibe A et al. Ethanol-Induced Mitochondrial Damage in Sertoli Cells is Associated with Parkin Overexpression and Activation of Mitophagy Cells Mar 25 2019 [PMID: 30934625] (IHC-P, Rat)
• Park JS, Lee DH, Lee YS et al. Dual roles of ULK1 (unc-51 like autophagy activating kinase 1) in cytoprotection against lipotoxicity Autophagy Mar 25 2019 [PMID: 30907226] (WB, Human)
• Cairo M, Campderros L, Gavalda-Navarro A et al. Parkin controls brown adipose tissue plasticity in response to adaptive thermogenesis EMBO Rep. Mar 13 2019 [PMID: 30867164] (WB, Mouse)
• Park H, Chung KM, An HK et al. Parkin Promotes Mitophagic Cell Death in Adult Hippocampal Neural Stem Cells Following Insulin Withdrawal Front Mol Neurosci. 2019 Feb 21 [PMID: 30853892] (ICC/IF, Human)
• McWilliams TG, Barini E, Pohjolan-Pirhonen R et al. Phosphorylation of Parkin at serine 65 is essential for its activation in vivo. Open Biol. [PMID: 30404819] (Human)
• Kim HJ, Joe Y, Rah SY et al. Carbon monoxide-induced TFEB nuclear translocation enhances mitophagy/mitochondrial biogenesis in hepatocytes and ameliorates inflammatory liver injury. Cell Death Dis. Oct 17 2018 [PMID: 30333475] (WB, Mouse)
• Civiletto G, Dogan SA, Cerutti R et al. Rapamycin rescues mitochondrial myopathy via coordinated activation of autophagy and lysosomal biogenesis. EMBO Mol Med. Oct 11 2018 [PMID: 30309855] (WB, Mouse)
• Araya J, Tsubouchi k, Sato N et al. PRkN-regulated mitophagy and cellular senescence during COPD pathogenesis. Autophagy. Oct 5 2018 [PMID: 30290714] (WB, Human)
• Walsh TG, van den Bosch MTJ, Lewis KE et al. Loss of the mitochondrial kinase PINK1 does not alter platelet function. Sci Rep Sep 26 2018 [PMID: 30258205] (WB, Mouse)
• Sun X, Duan Y, Qin C et al. Distinct multilevel misregulations of Parkin and PINK1 revealed in cell and animal models of TDP-43 proteinopathy. Cell Death Dis Sep 20 2018 [PMID: 30237395] (WB, Drosophila)
• Basso V, Marchesan E, Peggion C et al. Regulation of Endoplasmic Reticulum-Mitochondria contacts by Parkin via Mfn2. Pharmacol. Res. Sep 13 2018 [PMID: 30219582] (WB, Mouse)
• Thomas HE, Zhang Y, Stefely JA et al. Mitochondrial Complex I Activity Is Required for Maximal Autophagy Cell Rep Aug 28 2018 [PMID: 30157433] (WB, Human)
• Kumar A, Shaha C. SESN2 facilitates mitophagy by helping Parkin translocation through ULK1 mediated Beclin1 phosphorylation Sci Rep 2018 Jan 12 [PMID: 29330382] (WB, Human)
• Morris HR The PINK1-Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells bioRxiv Jan 1 2020 (WB, Human)
• Xu G, Li T, Chen J et al. Autosomal dominant retinitis pigmentosa-associated gene PRPF8 is essential for hypoxia-induced mitophagy through regulating ULK1 mRNA splicing Autophagy Aug 13 2018 [PMID: 30103670] (WB, Human)
• Yakhine-Diop SMS, Niso-Santano M, Rodriguez-Arribas M et al. Impaired Mitophagy and Protein Acetylation Levels in Fibroblasts from Parkinson's Disease Patients. Mol. Neurobiol. Jul 21 2018 [PMID: 30032424] (Human)
• Marcassa E, Kallinos A, Jardine J et al. Dual role of USP30 in controlling basal pexophagy and mitophagy EMBO Rep. Jun 12 2018 [PMID: 29895712] (Human)
• Sun Y, Yao X, Zhang QJ et al. Beclin-1-Dependent Autophagy Protects the Heart During Sepsis Circulation May 31 2018 [PMID: 29853517] (Mouse)
• Gu X, Qi Y, Feng Z et al. Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway. Toxicol. Lett. Apr 13 2018 [PMID: 29660402] (WB)
• Hawk MA, Gorsuch CL, Fagan P, Lee C. RIPK1-mediated induction of mitophagy compromises the viability of extracellular-matrix-detached cells. Nat. Cell Biol. 2018 Mar 01 [PMID: 29459781] (WB)
• Biel TG, Rao VA. Mitochondrial dysfunction activates lysosomal-dependent mitophagy selectively in cancer cells. Oncotarget. 2018 Jan 02 [PMID: 29416672] (WB, Human)
• Bueno M, Brands J, Voltz L et al. ATF3 represses PINK1 gene transcription in lung epithelial cells to control mitochondrial homeostasis Aging Cell 2018 Jan 24 [PMID: 29363258] (WB, Mouse)
• Goiran T, Duplan E, Rouland L et al. Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation Cell Death Differ. May 1 2018 [PMID: 29352272] (WB, Mouse)
• Greiner GF, Conraux C, Collard M, Picart P. [Value of pendular rotational methods in the study of vestibular reequilibration]. Rev Otoneuroophtalmol 2018 [PMID: 29290944] (WB, Human)
• Yoo L, Chung KC. The ubiquitin E3 ligase CHIP promotes proteasomal degradation of the serine/threonine protein kinase PINK1 during staurosporine-induced cell death. J. Biol. Chem. 2017 Dec 14 [PMID: 29242192] (Human)
• Sorrentino V, Romani M, Mouchiroud L et al. Enhancing mitochondrial proteostasis reduces amyloid-B proteotoxicity. Nature 2017 Dec 14 [PMID: 29211722] (Human)
• Mizumura K, Justice MJ, Schweitzer KS et al. Sphingolipid regulation of lung epithelial cell mitophagy and necroptosis during cigarette smoke exposure. FASEB J. 2017 Dec 01 [PMID: 29196503] (Mouse)
• Tang C, Han H, Yan M et al. PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against Renal ischemia-reperfusion injury Autophagy. 2017 Nov 26 [PMID: 29172924] (WB)
• Martin-Maestro P, Gargini R, A Sproul A et al. Mitophagy Failure in Fibroblasts and iPSC-Derived Neurons of Alzheimer's Disease-Associated Presenilin 1 Mutation Front Mol Neurosci 2017 Sep 29 [PMID: 28959184] (Human)
• Martinez-Vicente M, Franco-Iborra S, Plaza-Zabala A et al. Impairment of neuronal mitophagy by mutant huntingtin bioRxiv May 24 2018
• Kravic B, Harbauer AB, Romanello V et al. In mammalian skeletal muscle, phosphorylation of TOMM22 by protein kinase CSNK2/CK2 controls mitophagy Autophagy. 2017 Nov 22 [PMID: 29165030] (Mouse)
• Kim H, Ham S, Jo M et al. CRISPR-Cas9 Mediated Telomere Removal Leads to Mitochondrial Stress and Protein Aggregation Int J Mol Sci. 2017 Oct 03 [PMID: 28972555] (WB, Human)
• Bartolome A, Garcia-Aguilar A, Asahara SI et al. MTORC1 Regulates both General Autophagy and Mitophagy Induction after Oxidative Phosphorylation Uncoupling Mol. Cell. Biol. 2017 Sep 11 [PMID: 28894028] (WB)
• Xiao B, Goh JY, Xiao L et al. Reactive oxygen species trigger Parkin/PINK1 pathway-dependent mitophagy by inducing mitochondrial recruitment of Parkin J. Biol. Chem. 2017 Aug 28 [PMID: 28848050] (WB)
• Zhang C, Liu Z, Bunker E et al. Sorafenib Targets the Mitochondrial Electron Transport Chain Complexes and ATP Synthase to Activate the PINK1-Parkin Pathway and Modulate Cellular Drug Response J. Biol. Chem. 2017 Jul 03 [PMID: 28673964] (WB)
• Ando M, Fiesel FC, Hudec R et al. The PINK1 p.I368N mutation affects protein stability and ubiquitin kinase activity. Mol Neurodegener. Apr 24 2017 [PMID: 28438176] (WB, ICC/IF, Human)
• Orr AL, Rutaganira FU, de Roulet D et al. Long-term oral kinetin does not protect against alpha-synuclein-induced neurodegeneration in rodent models of Parkinson's disease. Neurochem. Int. Apr 20 2017 [PMID: 28434973] (WB)
• Martinez A, Lectez B, Ramirez J et al. Quantitative proteomic analysis of Parkin substrates in Drosophila neurons. Mol Neurodegener. 2017 Apr 11 [PMID: 28399880] (WB, Human)
• Gelmetti V, DeRosa P, Torosantucci L et al. PINK1 and BECN1 relocalize at mitochondria-associated membranes during mitophagy and promote ER-mitochondria tethering and autophagosome formation. Autophagy. Dec 23 2016 [PMID: 28368777] (ICC/IF, WB, IP, Human)
• Wang J, Zhu S, Wang Y et al. Miro2 supplies a platform for Parkin translocation to damaged mitochondria Science Bulletin 2019 Jun 01 (IP, WB, Knockdown, Mouse, Human)
• Sato S, Furuya N. Induction of PINK1/Parkin-Mediated Mitophagy. Methods Mol. Biol. Mar 31 2017 [PMID: 28361482]
• Berndsen K, Lis P, Yeshaw W et al. PPM1H phosphatase counteracts LRRK2 signaling by selectively dephosphorylating Rab proteins bioRxiv Jul 22 2019
• Li GB, Fu RQ, Shen HM et al. Polyphyllin I induces mitophagic and apoptotic cell death in human breast cancer cells by increasing mitochondrial PINK1 levels. Oncotarget. Feb 7 2017 [PMID: 28060722] (WB, Human)
• Wettengel J, Reautschnig P, Geisler S et al. Harnessing human ADAR2 for RNA repair-Recoding a PINK1 mutation rescues mitophagy. Nucleic Acids Research Oct 7 2016 [PMID: 27907896] (IHC, Human)
• Callegari S, Oeljeklaus S, Warscheid B et al. Phospho-ubiquitin-PARK2 complex as a marker for mitophagy defects. Autophagy. Nov 15 2016 [PMID: 27846363]
• Jamil S, Hojabrpour P, Duronio V. The small molecule 2-phenylethynesulfonamide induces covalent modification of p53. Biochem. Biophys. Res. Commun. Nov 7 2016 [PMID: 27833016] (WB, Human)
• Wai T, Saita S, Nolte H et al. The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L. EMBO Rep. Oct 13 2016 [PMID: 27737933]
• Mannam P, Rauniyar N, Lam TT et al. MKK3 influences mitophagy and is involved in cigarette smoke-induced inflammation. Free Radic Biol Med 2016 Dec [PMID: 27717867]
• Zhang T, Xue L, Li L et al. BNIP3 Suppresses PINK1 Proteolytic Cleavage to Promote Mitophagy. J. Biol. Chem. Aug 15 2016 [PMID: 27528605] (IP, ICC/IF)
• Xu Y, Zhi F, Shao N et al. Cytoprotection against Hypoxic and/or MPP+ Injury: Effect of delta-Opioid Receptor Activation on Caspase 3. Int J Mol Sci. Aug 9 2016 [PMID: 27517901] (WB)
• Kim J, Fiesel FC, Belmonte KC et al. miR-27a and miR-27b regulate autophagic clearance of damaged mitochondria by targeting PTEN-induced putative kinase 1 (PINK1) Mol Neurodegener 2016 Jul 26 [PMID: 27456084] (WB)
• Akabane S, Matsuzaki K, Yamashita SI et al. Constitutive Activation of PINK1 leads to Proteasome-Mediated and Non-apoptotic Cell Death Independently of Mitochondrial Autophagy. J. Biol. Chem. Jun 14 2016 [PMID: 27302064] (Human)
• Scott TL, Wicker CA, Suganya R et al. Polyubiquitination of apurinic/apyrimidinic endonuclease 1 by Parkin. Mol. Carcinog. May 5 2016 [PMID: 27148961]
• Berezhnov AV, Soutar MP, Fedotova EI et al. Intracellular pH Modulates Autophagy and Mitophagy. J Biol Chem 2016 Apr 15 [PMID: 26893374]
• Martin-Maestro P, Gargini R, Perry G et al. PARK2 enhancement is able to compensate mitophagy alterations found in sporadic Alzheimer's disease. Hum. Mol. Genet. 2015 Dec 31 [PMID: 26721933] (WB, ICC/IF, Human)
• Qu D, Hage A, Don-Carolis K et al. Bag2 Mediated Regulation of Pink1 is Critical for Mitochondrial Translocation of Parkin and Neuronal Survival. J. Biol. Chem. 2015 Nov 04 [PMID: 26538564] (WB, IP, Human)
• Ivankovic D, Chau KY, Schapira AH, Gegg ME. Mitochondrial and lysosomal biogenesis are activated following PINK1/parkin-mediated mitophagy. J. Neurochem. 2015 Oct 28 [PMID: 26509433] (WB, Human)
  
  Details:
  PINK1 antibody was used for WB analysis of 10 uM carbonylcyanide m-chlorophenylhydrazone /CCCP treated SH-SY5Y cells which were expressing scrambled shRNA or PINK1shRNA namely 117-2, 118-3, and 118-4 (Fig 3a).
• Fiesel FC, Ando M, Hudec R et al. (Patho-)physiological relevance of PINK1-dependent ubiquitin phosphorylation. EMBO Rep 2015 Sep [PMID: 26162776] (WB)
• Srivastava A, McGinniss J, Wong Y et al. MKK3 deletion improves mitochondrial quality Free Radic. Biol. Med. 2015 Jun 25 [PMID: 26119780] (WB, Mouse)
• Meissner C, Lorenz H, Hehn B, Lemberg MK. Intramembrane protease PARL defines a negative regulator of PINK1- and PARK2/Parkin-dependent mitophagy Autophagy. 2015 Jun 23 [PMID: 26101826] (WB, Human)
• Park S, Choi SG, Yoo SM et al. Pyruvate stimulates mitophagy via PINK1 stabilization Cell. Signal. 2015 Jun 09 [PMID: 26071202] (WB, Human)
• Peng M, Ostrovsky J, Kwon YJ et al. Inhibiting cytosolic translation and autophagy improves health in mitochondrial disease Hum. Mol. Genet. 2015 Jun 03 [PMID: 26041819] (WB, Human)
• Lim GG, Chua DS, Basil AH et al. Cytosolic PTEN-INDUCED PUTATIVE KINASE 1 is Stabilized By NF-kB Pathway and Promotes Non-Selective Mitophagy. J. Biol. Chem. 2015 May 18 [PMID: 25987559] (ICC/IF, IP, Human)
• Okatsu K, Koyano F, Kimura M et al. Phosphorylated ubiquitin chain is the genuine Parkin receptor J. Cell Biol. 2015 Apr 13 [PMID: 25847540] (WB, Human)
• Yue M, Hinkle KM, Davies P et al. Progressive dopaminergic alterations and mitochondrial abnormalities in LRRK2 G2019S knock-in mice Neurobiol. Dis. 2015 Mar 31 [PMID: 25836420] (WB, Mouse)
• Seillier M, Pouyet L, N'Guessan P et al. Defects in mitophagy promote redox-driven metabolic syndrome in the absence of TP53INP1 EMBO Mol Med 2015 Mar 30 [PMID: 25828351] (WB, Human, Mouse)
• Han JY, Kang MJ, Kim KH et al. Nitric Oxide Induction of Parkin Translocation in PTEN-Induced Putative Kinase 1 (PINK1) Deficiency: Functional Role of Neuronal Nitric Oxide Synthase During Mitophagy J. Biol. Chem. 2015 Feb 25 [PMID: 25716315] (WB, Human)
• Jabir MS, Hopkins L, Ritchie ND et al. Mitochondrial damage contributes to Pseudomonas aeruginosa activation of the inflammasome and is downregulated by autophagy. Autophagy 2015 [PMID: 25700738] (WB)
• Bronner DN. Cells on Fire: Regulation of the NLRP3 inflammasome via endoplasmic reticulum and mitochondrial crosstalk. Thesis. 2015 (WB, Mouse)
• Holmstrom K. The Influence of the PD-associated Gene Products PINK1 and HtrA2/Omi on Mitochondrial Integrity Dissertation http://nbn-resolving.de/urn:nbn:de:bsz:21-opus-48153 2010 May 21 (WB, Human)
• Palubinsky AM, Stankowski JN, Kale AC et al. CHIP is an Essential Determinant of Neuronal Mitochondrial Stress Signaling Antioxid. Redox Signal. 2015 Jan 20 [PMID: 25602369] (WB, ICC/IF, Mouse, Rat)
  
  Details:
  PINK1 antibody used for WB on whole brain lysates of PND35 CHIP/C-terminus of HSC70-interacting protein knockout mouse model (FIG 2A) and on whole cell lysates of neuronal rat cultures (DIV 21-25) harvested at different recovery time frames following exposure to oxygen-glucose deprivation/OGD (FIG 3A). PINK1 antibody was also used at 1:500 dilution for ICC-IF application on neuronal rat cultures (DIV 21-25) subjected to 90 minutes of OGD followed by 6 hours of recovery (FIG. 4C).
• Bueno M, Lai Y, Romero Y et al. PINK1 deficiency impairs mitochondrial homeostasis and promotes lung fibrosis. J. Clin. Invest. 2014 Dec 22 [PMID: 25562319] (WB, Mouse)
• Aerts L, Craessaerts K, De Strooper B, Morais VA. PINK1 Catalytic Activity is Regulated by Phosphorylation on Serines 228 and 402. J. Biol. Chem. 2014 Dec 19 [PMID: 25527497] (WB)
• Bifsha P, Yang J, Fisher RA, Drouin J. Rgs6 is Required for Adult Maintenance of Dopaminergic Neurons in the Ventral Substantia Nigra. PLoS Genet. 2014 Dec 01 [PMID: 25501001] (IHC-P, Mouse)
• Shiba-Fukushima K, Arano T, Matsumoto G et al. Phosphorylation of Mitochondrial Polyubiquitin by PINK1 Promotes Parkin Mitochondrial Tethering. PLoS Genet. 2014 Dec 01 [PMID: 25474007] (WB, Human)
• Lee S, Zhang C, Liu X. Role of Glucose Metabolism and ATP in Maintaining PInK1 Levels During Parkin-mediated Mitochondrial Damage Responses. J. Biol. Chem. 2014 Nov 17 [PMID: 25404737] (WB, Human, Mouse)
• Shiba-Fukushima K, Inoshita T, Hattori N et al. Lysine 63-Linked Polyubiquitination Is Dispensable for Parkin-Mediated Mitophagy. J. Biol. Chem. 2014 Oct 21 [PMID: 25336644] (WB, Human)
• Choi Hk, Choi Y, Kang H et al. PInK1 positively regulates HDAC3 to suppress dopaminergic neuronal cell death. Hum. Mol. Genet. 2014 Oct 09 [PMID: 25305081] (IP, Human)
• Grenier K, Kontogiannea M, Fon EA. Short Mitochondrial ARF Triggers Parkin/PINK1-Dependent Mitophagy. J Biol Chem. 2014 Sep 12 [PMID: 25217637]
• Duan Xiaoling, Tong Jade, Xu Qin et al. Upregulation of human PINK1 gene expression by NFkappaB signalling. Mol Brain. 2014 Aug 11 [PMID: 25108683] (WB)
• Mizumura K, Cloonan SM, Nakahira K et al. Mitophagy-dependent necroptosis contributes to the pathogenesis of COPD. J Clin. Invest. 2014 Aug 01 [PMID: 25083992] (IHC-P, Human)
• Rossin F, D'Eletto M, Falasca L et al. Transglutaminase 2 ablation leads to mitophagy impairment associated with a metabolic shift towards aerobic glycolysis. Cell Death Differ 2015 Mar [PMID: 25060553] (WB)
• Fiesel FC, Moussaud-LamodiEre EL, Ando M, Springer W A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently J Cell Sci. 2014 Aug 14 [PMID: 24928900] (ICC/IF, KD, Human)
• Montaigne D, Marechal X, Coisne A et al. Myocardial Contractile Dysfunction is Associated with Impaired Mitochondrial Function and Dynamics in Type 2 Diabetic but not in Obese Patients. Circulation. 2014 Jun 13 [PMID: 24928681] (WB, Human)
• Choubey V, Cagalinec M, Liiv J et al. BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria. Autophagy 2014 Jun [PMID: 24879156] (WB)
• Patil KS, Basak I, Lee S et al. PARk13 regulates PINk1 and subcellular relocation patterns under oxidative stress in neurons. J. Neurosci. Res. 2014 May 02 [PMID: 24798695] (WB, Human)
• Koyano F, Okatsu K, Kosako H et al. Ubiquitin is phosphorylated by PINk1 to activate parkin. Nature. 2014 Apr 30 [PMID: 24784582] (WB, Human, Mouse)
• Parganlija D, Klinkenberg M, Dominguez-Bautista J et al. Loss of PINK1 Impairs Stress-Induced Autophagy and Cell Survival. PLoS ONE 2014 Apr 22 [PMID: 24751806] (WB, Human)
• Kane LA, Lazarou M, Fogel AI et al. PINK1 phosphorylates ubiquitin to activate Parkin E3 ubiquitin ligase activity. J. Cell Biol. 2014 Apr 28 [PMID: 24751536] (WB, Human)
• Yamano K, Fogel AI, Wang C et al. Mitochondrial Rab GAPs govern autophagosome biogenesis during mitophagy Elife. 2014 Feb 24 [PMID: 24569479] (WB, KD, Human)
• McLelland GL, Soubannier V, Chen CX, Fon EA. Parkin and PINK1 function in a vesicular trafficking pathway regulating mitochondrial quality control. EMBO J. 2014 Feb 21 [PMID: 24446486] (WB, Human)
• Zhou ZD, Refai FS, Xie SP et al. Mutant PINK1 upregulates tyrosine hydroxylase and dopamine levels, leading to vulnerability of dopaminergic neurons. Free Radic Biol Med 2013 Dec 25 [PMID: 24374372] (WB, ICC/IF, Human)
• Okatsu K, Uno M, Koyano F et al. A dimeric PINK1-containing complex on depolarized mitochondria stimulates Parkin recruitment. J Biol Chem. 2013 Nov 4 [PMID: 24189060] (WB, Human)
• Gomez-Sanchez R, Gegg ME, Bravo-San Pedro JM et al. Mitochondrial impairment increases FL-PINK1 levels by calcium-dependent gene expression. Neurobiol. Dis. 2014 Feb 1 [PMID: 24184327] (WB, ICC/IF, Human)
• Dagda RK, Pien I, Wang R et al. Beyond the mitochondrion: cytosolic PINK1 remodels dendrites through Protein Kinase A. J Neurochem. 2013 Oct 23 [PMID: 24151868] (WB, Human)
• Jin SM, Youle RJ. The accumulation of misfolded proteins in the mitochondrial matrix is sensed by PINK1 to induce PARK2/Parkin-mediated mitophagy of polarized mitochondria. Autophagy 2013 Nov 1 [PMID: 24149988] (WB)
• Yamano K, Youle RJ. PINK1 is degraded through the N-end rule pathway. Autophagy 2013 Nov 1 [PMID: 24121706]
• Hoshino A, Mita Y, Okawa Y et al. Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart. Nat Commun 2013 Aug 6 [PMID: 23917356] (WB, Mouse)
• Murata H, Sakaguchi M, Kataoka K, Huh NH. SARM1 and TRAF6 bind to and stabilize PINK1 on depolarized mitochondria. Mol Biol Cell 2013 Jul 24 [PMID: 23885119] (WB, Human)
• Iguchi M, Kujuro Y, Okatsu K et al. Parkin-catalyzed Ubiquitin-Ester Transfer Is Triggered by PINK1-dependent Phosphorylation. J Biol Chem 2013 Jul 26 [PMID: 23754282] (WB, Mouse)
• Koyano F, Okatsu K, Ishigaki S et al. The principal PINK1 and Parkin cellular events triggered in response to dissipation of mitochondrial membrane potential occur in primary neurons. Genes Cells 2013 Jun 10 [PMID: 23751051] (WB, Mouse)
• Narendra DP, Wang C, Youle RJ, Walker JE. PINK1 rendered temperature sensitive by disease-associated and engineered mutations Hum Mol Genet 2013 Mar 15 [PMID: 23459931] (WB, Human)
• Saita S, Shirane M, Nakayama KI. Selective escape of proteins from the mitochondria during mitophagy. Nat Commun 2013 Jan 29 [PMID: 23361001] (WB, Mouse)
• Lazarou Michael, Narendra Derek P, Jin Seok Min et al. PINK1 drives Parkin self-association and HECT-like E3 activity upstream of mitochondrial binding. J Cell Biol 2013 [PMID: 23319602] (WB, Human)
• Song S, Jang S, Park J et al. Characterization of phosphatase and tensin homolog (PTEN)-induced putative kinase 1 (PINK1) mutations associated with Parkinson's disease in mammalian cells and Drosophila J Biol Chem 2013 Jan 9 [PMID: 23303188] (ICC/IF, WB, Human)
• Shiba-Fukushima K, Imai Y, Yoshida S et al. PINK1-mediated phosphorylation of the Parkin ubiquitin-like domain primes mitochondrial translocation of Parkin and regulates mitophagy Sci Rep 2012 [PMID: 23256036] (WB, Mouse)
• Wood-Kaczmar A, Deas E, Wood NW, Abramov AY. The Role of the Mitochondrial NCX in the Mechanism of Neurodegeneration in Parkinson's Disease Adv Exp Med Biol 2013 [PMID: 23224884] (ICC/IF, Human)
• Sekine S, Kanamaru Y, Koike M et al. Rhomboid Protease PARL Mediates the Mitochondrial Membrane Potential Loss-induced Cleavage of PGAM5 J Biol Chem 2012 Oct 5 [PMID: 22915595]
• Okatsu K, Oka T, Iguchi M et al. PINK1 autophosphorylation upon membrane potential dissipation is essential for Parkin recruitment to damaged mitochondria Nat Commun 2012 Aug 21 [PMID: 22910362] (WB, ICC/IF, Human, Mouse)
• Kondapalli C, Kazlauskaite A, Zhang N et al. PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65 Open Biol 2012 May [PMID: 22724072] (WB, Human)
• Becker D, Richter J, Tocilescu MA et al. Pink1 kinase and its membrane potential (Delta-psi)-dependent cleavage product both localize to outer mitochondrial membrane by unique targeting mode. J Biol Chem. 2012 Jun 29 [PMID: 22547060] (WB, ICC/IF, Human)
• Wang X, Winter D, Ashrafi G et al. PINK1 and Parkin target Miro for phosphorylation and degradation to arrest mitochondrial motility. Cell. 2011 Nov 11 [PMID: 22078885] (WB, IP, Human)
• Mitsuhashi S, Hatakeyama H, Karahashi M et al. Muscle choline kinase beta defect causes mitochondrial dysfunction and increased mitophagy Hum Mol Genet 2011 Oct [PMID: 21750112] (IHC, ICC/IF, Mouse)
• Meissner C, Lorenz H, Weihofen A et al. The mitochondrial intramembrane protease PARL cleaves human Pink1 to regulate Pink1 trafficking. J Neurochem. 2011 Jun. [PMID: 21426348] (WB, Human)
• Imai Y, Kanao T, Sawada T et al. The loss of PGAM5 suppresses the mitochondrial degeneration caused by inactivation of PINK1 in Drosophila. PLoS Genet. 2010 Dec 2 [PMID: 21151955] (WB, ICC/IF, Human)
• Lin W, Kang UJ. Structural determinants of PINK1 topology and dual subcellular distribution. BMC Cell Biol 2010 Nov 22 [PMID: 21092208]
• Kamp F, Exner N, Lutz AK et al. Inhibition of mitochondrial fusion by alpha-synuclein is rescued by PINK1, Parkin and DJ-1. EMBO J. 2010 Oct 20 [PMID: 20842103] (WB, Human)
• Geisler S, Holmstrom KM, Treis A et al. The PINK1/Parkin-mediated mitophagy is compromised by PD-associated mutations. Autophagy 2010 Oct [PMID: 20798600] (WB, Human)
• Cui J, Yao Q, Li S et al. Glutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family. Science;329(5996):1215-8. 2010 Sep 3. [PMID: 20688984] (WB, Human)
• Irrcher I, Aleyasin H, Seifert EL et al. Loss of the Parkinson's disease-linked gene DJ-1 perturbs mitochondrial dynamics Hum Mol Genet 2010 Oct 1 [PMID: 20639397] (WB, Mouse)
• M Cui, X Tang, W V Christian et al. Perturbations in Mitochondrial Dynamics Induced by Human Mutant PINK1 Can Be Rescued by the Mitochondrial Division Inhibitor mdivi-1. J Biol Chem; 285 (15): 11740-11752. 2010 [PMID: 20164189] (WB, Rat)
• Gegg ME, Cooper JM, Schapira AH, Taanman JW. Silencing of PINK1 expression affects mitochondrial DNA and oxidative phosphorylation in dopaminergic cells. PLoS One 2009 [PMID: 19270741] (WB, Human)
• Liu W, Vives-Bauza C, Acin-Perez- R et al. PINK1 defect causes mitochondrial dysfunction, proteasomal deficit and alpha-synuclein aggregation in cell culture models of Parkinson's disease. PLoS One;4(2):e4597. 2009 [PMID: 19242547] (IP, WB, Human)
• Weihofen A, Thomas KJ, Ostaszewski BL et al. Pink1 forms a multiprotein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking. Biochemistry 2009 Mar 10 [PMID: 19152501] (WB)
• Zhou C, Huang Y, Shao Y et al. The kinase domain of mitochondrial PINK1 faces the cytoplasm. Proc Natl Acad Sci U S A;105(33):12022-7. 2008 Aug 19. [PMID: 18687899] (ICC/IF, WB, Human)
• Lin W, Kang UJ. Characterization of PINK1 processing, stability, and subcellular localization J Neurochem 2008 Jul [PMID: 18397367] (WB, Human)
• Weihofen, A, B Ostaszewski et al. Pink1 Parkinson mutations, the Cdc37/Hsp90 chaperones and Parkin all influence the maturation or subcellular distribution of Pink1. Hum Mol Genet;17(4):602-16. 2008 Feb 15. [PMID: 18003639] (WB, Human)
• Exner, N et al. Loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by parkin. J Neurosci;27(45):12413-8. 2007 Nov 7. [PMID: 17989306] (WB, Human)
• Lin W, Kang UJ. Characterization of PINK1 processing, stability, and subcellular localization. J Neurochem;106(1):464-74. 2008 Jul. [PMID: 1839736] (Human)

Reviews for PINK1 Antibody (BC100-494) (11)

Average Rating: 3.7

(Based on 11 reviews)

We have 11 reviews tested in 4 species: Human, Mouse, Human, Rat, Mouse, Rabbit.

reviewed by: Anonymous WB Mouse 03/23/2019

Summary

• Application: Western Blot
• Sample Tested: mouse spinal cord
• Species: Mouse
• Lot: G-1

Comments

• Comments: Blocked with 5% BSA in PSB-T. Primary antibody at 1:1000 overnight at 4 degrees followed by secondary antibody at 1:10,000 for 1 hour at room temperature.

reviewed by: Anonymous WB Mouse 12/21/2018

Summary

• Application: Western Blot
• Sample Tested: mouse vascular smooth muscle cell
• Species: Mouse
• Lot: M

reviewed by: seulgee lee Rabbit 04/24/2018

Summary

• Sample Tested: Heart tissue
• Species: Rabbit
• Lot: G-1

reviewed by: Elena Marcassa WB Human 02/05/2018

Summary

• Application: Western Blot
• Sample Tested: hTERT RPE-1 cell line from ATCC,U2OS cell line,IMCD3, hTertRPE1, HDF, SHSY5Y whole cell lysates
• Species: Human
• Lot: K-5

reviewed by: Anonymous ELISA Human, Rat, Mouse 01/29/2018

Summary

• Application: ELISA
• Sample Tested: mouse corneal whole-mount immunofluorescence staining,ELISA Sample Tested
• Species: Human, Rat, Mouse
• Lot: AB_10127658

reviewed by: Anonymous WB Human 01/20/2017

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human

reviewed by: Anonymous WB Mouse 08/06/2016

Summary

• Application: Western Blot
• Species: Mouse
• Lot: PO number: E0513646
• File: View PDF

reviewed by: Anonymous WB Human 03/31/2016

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human

Blocking

• Blocking Details: 5% milk in PBS, followed by a rinse in PBS-tween, 30min at room temperature with rocking

Primary Anitbody

• Dilution Ratio: 1/1000 in 4% BSA in PBS, incubation for 1h at room temperature and mild rocking

Secondary Antibody

• Secondary Description: Anti-Rabbit IgG (H+L), HRP Conjugate
• Secondary Manufacturer Cat#: w4011 (Promega)
• Secondary Concentration: 1/5000 in 5% milk in PBS

Details

• Detection Notes: FluorChem M

Comments

• Comments: Occasionally observe a non-specific band at ~17kD

reviewed by: Shireen Sarraf WB Human 07/05/2011

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate, Sample Amount: 60ug
• Species: Human
• Comments: This is one of the better PINK1 antibodies available, although there are a lot of background bands and the PINK1 signal is not strong. It is easier to observe the PINK1 bands if the non-specific bands are cut-off before antibody incubation. There is a strong band around 29kd.

Blocking

• Blocking Details: Blocking Buffer: Li-COR blocking buffer, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:1000, Incubation Dilution Buffer: Li-COR blocking buffer, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

• Secondary Description: Secondary Ab: Li-COR IRdye 800CW, Secondary Ab Dilution Ratio: 1:10000
• Secondary Manufacturer Cat#: 926-32211

Details

• Detection Notes: Detection:Li-COR, Pos.Cnt’l: Chemical treatment, Neg.Cnt’l:PINK1 siRNA, Bands:67,55,45kDa(specific)&116,70,29kDa(non-specific)

Comments

• Comments: This is one of the better PINK1 antibodies available, although there are a lot of background bands and the PINK1 signal is not strong. It is easier to observe the PINK1 bands if the non-specific bands are cut-off before antibody incubation. There is a strong band around 29kd.

reviewed by: Adam Elwi WB Human 11/10/2010

Summary

• Application: Western Blot
• Sample Tested: HeLa whole cell lysate, Sample Amount: 20ug
• Species: Human

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: anti-Rabbit IgG HRP-conjugated, Secondary Ab Dilution Ratio: 1:2000

Details

• Detection Notes: Detection Method: Amersham ECL, Negative Control: HeLa K/O PINK1, Specific Bands: expected

reviewed by: William Lin WB Human 05/17/2010

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: A32
• Pub Med ID: 18397367
• File: View PDF

Blocking

• Blocking Details: Blocking buffer: 5% milk; Blocking concentration: 1:1000; Blocking time: 1 hour at room temp; Blocking temp: 25C Celsius

Primary Anitbody

• Dilution Ratio: Primary antibody dilution: 1:1000; Incubation time: overnight; Incubation temp: 4C Celsius; Incubation buffer: 5% milk; Sample amount: 30ug

Secondary Antibody

• Secondary Description: mouse HRP
• Secondary Manufacturer Cat#: sigma
• Secondary Concentration: 1:5000

Details

• Detection Notes: Detection method: chemiluminescence; Exposure time: 5 minutes;

FAQs for PINK1 Antibody (BC100-494). (Showing 1 - 4 of 4 FAQs).

1. We recently purchased BC100-494 and the HEK293 cell extract to serve as a positive control. The most prominent band I got is approximately 120 kDa. However, after the cells were treated with DMSO, the most intense band became approximately 60 and 50 kDa (like the datasheet). Since there are some references that mention that PINK1 can form dimer or associate with other mitochondrial proteins, I am wondering if the reason of 120 kDa band is due to the strong binding of protein complex?
   • It is most likely being picked up also at 120 kDa due to associations with other proteins. Our lab has not characterized this higher molecular weight band, but this seems like a likely explanation based on some publications I have found. One which you might find useful is The Mitochondrial Fusion-Promoting Factor Mitofusin Is a Substrate of the PINK1/Parkin Pathway. I recommend trying a negative control competition assay with BC100-494PEP. This blocking peptide can help you determine if this 120 kDa band is specific. You can find a protocol for this procedure that we recommend at the following link, peptide competition protocol.
2. We would like to check toxicity of BC100-494. Could you inform us of the concentration of sodium azide?
   • This product contains 0.05% NaN3 as the preservative.
3. I ordered PINK1 antibody(BC100-494) at about six months ago. After it shipped to my country, it has been stored at -20 degrees Celsius in the agent company ever since. I recently called the agent to deliver it to me, and that's when I found this product has been wrongly stored according to the datasheet. So what I am worrying now is that does this influence the efficiency of this antibody? If it does, how much damage has been caused? What should I do to reduce the bad effects? Should I store this antibody by small packages?
   • We recommend that BC100-494 is stored at 4C and that it should not be frozen. Unfortunately we have no testing data regarding the use of the antibody following -20C storage, and as such I cannot guarantee that it will perform optimally now that it has been frozen. I suggest that you try using the antibody as usual, but perhaps at a higher (more concentrated) dilution than recommended in case its potency has been lost during the freezing process. I would store it at 4C from now on, and not freeze it again once you have thawed it, since repeated freeze-that cycles can be detrimental to performance.
4. Have you tried PINK1 antibody for immunoblotting in HEK293FT cells?
   • We have not performed any testing of this antibody in HEK293FT cells specifically. However, PINK1 is a widely expressed protein, and I would expect it to be present in that particular cell line (it is expressed in HEK293); however, you will most likely want to run this along side controls to confirm.

Bioinformatics

• Gene Symbol: PINK1
• Entrez:
  • 65018(Human)
• Uniprot:
  • Q9BXM7(Human)