PGAM1 Antibody - BSA Free

Western Blot: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in Jurkat whole cell extracts

Immunocytochemistry/Immunofluorescence: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in HeLa cells using NBP1-49532. Nuclei (Blue) are counterstained using Hoechst 33258.

Immunohistochemistry: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in mouse tongue.

Simple Western: PGAM1 Antibody [NBP1-49532] - Simple Western lane view shows a specific band for PGAM1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PGAM1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human PGAM1 protein (between residues 200-254) [UniProt P18669]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PGAM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
  • Knockdown Validated
  • Simple Western 1:400
  • Western Blot 0.5ug/ml
• Application Notes: This PGAM1 antibody may be used in Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockdown validation (PMID: 28122957).
• Control:

  Jurkat Whole Cell Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGAM1 Antibody - BSA Free

• BPG-dependent PGAM 1
• EC 3.1.3.13
• EC 5.4.2.1
• EC 5.4.2.4
• PGAMA
• PGAM-Bphosphoglycerate mutase 1
• phosphoglycerate mutase 1 (brain)
• phosphoglycerate mutase A, nonmuscle form
• Phosphoglycerate mutase isozyme B

Background

PGAM1 (phosphoglycerate mutase 1) is an enzyme that regulates a unique step in glycolysis, and most of the glycolytic intermediates that are used as precursors for anabolic biosynthesis are upstream of this step. PGAM1 catalyzes the interconversion of 3- phosphoglycerate (3-PGA) to 2- phosphoglycerate (2-PGA), and becomes activated or "primed" by an intrinsic phosphatase activity that converts 2,3-bisphosphoglycerate (2, 3-BPGA) to 2-PGA, phosphorylating the active site histidine in the process. The phosphohistidine is essential to carryout the mutase reaction, converting 3-PGA to 2-PGA in glycolysis. Interestingly, PGAM1 is the rate-limiting enzyme of glycolysis in tumor cells, heart tissue, and leukocytes, and its inhibition by epoxide inhibitors is lethal to cancer cells. In many cancers, including hepatocellular carcinoma and colorectal cancer, PGAM1 activity is increased compared to that in the normal tissues, wherein its expression is upregulated through loss of TP53 as TP53 negatively regulates PGAM1 gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGAM1 Antibody (NBP1-49532)(7)

Publications using NBP1-49532

• Alam H, Tang M, Maitituoheti M et al. KMT2D Deficiency Impairs Super-Enhancers to Confer a Glycolytic Vulnerability in Lung Cancer Cancer Cell 2020-04-13 [PMID: 32243837]
• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
• Sun Qian, Li Shuzhan, Wang Yanan et al. Phosphoglyceric acid mutase-1 contributes to oncogenic mTOR-mediated tumor growth and confers non-small cell lung cancer patients with poor prognosis. Cell Death and Differentiation 2018-01-01 [PMID: 29362480] (IHC, Human)
• Qu J, Sun W, Zhong J et al. Correction: Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells J. Cell Biol. 2017-07-24 [PMID: 28739677] (WB, Human)
  
  Details:
  Novus' PGAM1 antibbody was used to look at PGAM1 levels in HeLa cells that were either treated with 100uM dNTPs or not treated
• Qu J, Sun W, Zhong J et al. Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells J Cell Biol. 2016-07-03 [PMID: 28122957] (ICC/IF, KD, WB, Human)
• Liu Y, Cao Y, Zhang W et al. A small-molecule inhibitor of glucose transporter 1 downregulates glycolysis, induces cell-cycle arrest, and inhibits cancer cell growth in vitro and in vivo Mol Cancer Ther 2012-08-01 [PMID: 22689530] (WB, Human)
• di Domenico F The Role of Ankef1 in Male Fertility Thesis 2021-01-01 (WB)

Review for PGAM1 Antibody (NBP1-49532) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Anonymous - WB Other 09/21/2015

Summary

• Application: Western Blot
• Species: Other

FAQs for PGAM1 Antibody (NBP1-49532). (Showing 1 - 1 of 1 FAQs).

1. This antibody is reactive with human as noted in the datasheet. Can we use this antibody for dog tissues?
   • Our antibody NBP1-49532 has not yet been tested with dog PGAM1. However, I have run a UniProt Blast sequence comparison of the immunogen sequence (residues 200-254 of the human protein, UniProt P18669) with canine, and the result was 98% homology with canine PGAM1 (UniProt E2RT65). This strongly suggests that this antibody is suitable for use with dog tissues (although we cannot guarantee success). Note that if you decide to go ahead and test this antibody with a new species, you would be eligible for our Innovator's Reward. The details of this program are shown here: Innovator's Reward program.

Bioinformatics

• Gene Symbol: PGAM1
• Entrez:
  • 5223(Human)
  • 18648(Mouse)
• Uniprot:
  • Q9DBJ1(Mouse)