PGAM1 Antibody

Images

 
Western Blot: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in Jurkat whole cell extracts
Immunocytochemistry/ Immunofluorescence: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in HeLa cells using NBP1-49532. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in mouse tongue.
Simple Western: PGAM1 Antibody [NBP1-49532] - Simple Western lane view shows a specific band for PGAM1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

PGAM1 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human PGAM1 protein (between residues 200-254) [UniProt P18669]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PGAM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5ug/ml
  • Simple Western 1:400
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
Application Notes
This PGAM1 antibody may be used in Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Jurkat Lysate (NB800-PC2)
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49532 in the following applications:

Publications
Read Publications using
NBP1-49532 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PGAM1 Antibody

  • BPG-dependent PGAM 1
  • EC 3.1.3.13
  • EC 5.4.2.1
  • EC 5.4.2.4
  • PGAMA
  • PGAM-Bphosphoglycerate mutase 1
  • phosphoglycerate mutase 1 (brain)
  • phosphoglycerate mutase A, nonmuscle form
  • Phosphoglycerate mutase isozyme B

Background

PGAM1 (phosphoglycerate mutase 1) is an enzyme that regulates a unique step in glycolysis, and most of the glycolytic intermediates that are used as precursors for anabolic biosynthesis are upstream of this step. PGAM1 catalyzes the interconversion of 3- phosphoglycerate (3-PGA) to 2- phosphoglycerate (2-PGA), and becomes activated or "primed" by an intrinsic phosphatase activity that converts 2,3-bisphosphoglycerate (2, 3-BPGA) to 2-PGA, phosphorylating the active site histidine in the process. The phosphohistidine is essential to carryout the mutase reaction, converting 3-PGA to 2-PGA in glycolysis. Interestingly, PGAM1 is the rate-limiting enzyme of glycolysis in tumor cells, heart tissue, and leukocytes, and its inhibition by epoxide inhibitors is lethal to cancer cells. In many cancers, including hepatocellular carcinoma and colorectal cancer, PGAM1 activity is increased compared to that in the normal tissues, wherein its expression is upregulated through loss of TP53 as TP53 negatively regulates PGAM1 gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PGAM1 Antibody (NBP1-49532)(2)

Review for PGAM1 Antibody (NBP1-49532) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-49532:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Western Blot PGAM1 NBP1-49532
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reviewed by:
Anonymous
WB Other 09/21/2015
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Summary

ApplicationWestern Blot
SpeciesOther
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PGAM1 Antibody (NBP1-49532). (Showing 1 - 1 of 1 FAQs).

  1. This antibody is reactive with human as noted in the datasheet. Can we use this antibody for dog tissues?
    • Our antibody NBP1-49532 has not yet been tested with dog PGAM1. However, I have run a UniProt Blast sequence comparison of the immunogen sequence (residues 200-254 of the human protein, UniProt P18669) with canine, and the result was 98% homology with canine PGAM1 (UniProt E2RT65). This strongly suggests that this antibody is suitable for use with dog tissues (although we cannot guarantee success). Note that if you decide to go ahead and test this antibody with a new species, you would be eligible for our Innovator's Reward. The details of this program are shown here: Innovator's Reward program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49532AF405
Alexa Fluor 488 Labeled NBP1-49532AF488
Alexa Fluor 647 Labeled NBP1-49532AF647
Alexa Fluor 700 Labeled NBP1-49532AF700
Biotin Labeled NBP1-49532B
DyLight 405 Labeled NBP1-49532V
DyLight 488 Labeled NBP1-49532G
DyLight 550 Labeled NBP1-49532R
DyLight 650 Labeled NBP1-49532C
FITC Labeled NBP1-49532F
HRP Labeled NBP1-49532H

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Recent Reviews

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1
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Anonymous
09/21/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol PGAM1
Entrez
Uniprot