Carbonic Anhydrase II/CA2 Antibody

Images

 
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing ...read more
Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells ...read more
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - analysis of Carbonic Anhydrase II protein (loading amount: 50 ng) using rabbit anti-Carbonic Anhydrase II antibody (NB600-919) at a dilution of 1:1000, ...read more
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Analysis using the Biotin conjugate of NB600-919. Detection of Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic ...read more
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4C. ...read more
Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Localization of CAII in mouse peritoneum. Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

Carbonic Anhydrase II/CA2 Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Carbonic Anhydrase II/CA2 Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II/CA2. (Uniprot: P00918)
Localization
Cytoplasmic
Specificity
Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CA2
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:165000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:2000-1:10000
Application Notes
Carbonic Anhydrase II/CA2 Antibody is suitable for western blotting, ELISA and IHC. Expect ~29.2kDa. Researchers should determine optimal titers for applications that are not stated below.

Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24798518)
Theoretical MW
29.25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Carbonic Anhydrase II/CA2 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB600-919 in the following applications:

Publications
Read Publications using
NB600-919 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    6 publications

Reactivity Notes

Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Notes

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Carbonic Anhydrase II/CA2 Antibody

  • CA2
  • CAII
  • CA-IIEC 4.2.1.1
  • Car2
  • Carbonate dehydratase II
  • carbonic anhydrase 2
  • carbonic anhydrase B
  • Carbonic anhydrase C
  • Carbonic Anhydrase II
  • carbonic anhydrase IICAC
  • carbonic dehydratase

Background

Carbonic Anhydrase II Antibody detects Carbonic Anhydrase II. Carbonic Anhydrase II is essential for bone resorption and osteoclast differentiation by similarity. It can hydrate cyanamide to urea and is involved in the regulation of fluid secretion into the anterior chamber of the eye. Anti-Carbonic Anhydrase II Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase II/CA2 Antibody (NB600-919)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, WB.


Filter By Application
IHC
(1)
IHC-Fr
(1)
WB
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB600-919 Applications Species
Dai, S Y & Yang, D. A Visible and Near-Infrared Light Activatable Diazocoumarin Probe for Fluorogenic Protein Labeling in Living Cells. J Am Chem Soc Oct 7 2020 [PMID: 32870680] (IHC, WB, Human, Mouse) IHC, WB Human, Mouse
Mboge, M Y, Chen, Z Et al. A non-catalytic function of carbonic anhydrase IX contributes to the glycolytic phenotype and pH regulation in human breast cancer cells. Biochem J May 28 2019 [PMID: 31072911] (WB, Human) WB Human
Chen Z, Ai L, Mboge MY et al. Differential expression and function of CAIX and CAXII in breast cancer: A comparison between tumorgraft models and cells. PLoS ONE. Jul 2 2018 [PMID: 29965974] (WB, Mouse) WB Mouse
Yin J, Tse CM, Avula LR et al. Molecular Basis and Differentiation-Associated Alterations of Anion Secretion in Human Duodenal Enteroid Monolayers Cell Mol Gastroenterol Hepatol Feb 9 2018 [PMID: 29930980] (WB, Human) WB Human
Chen Z, Ai L, Mboge MY et al. UFH-001 cells: A novel triple negative, CAIX-positive, human breast cancer model system. Cancer Biol. Ther. Mar 21 2018 [PMID: 29561695] (WB, Human) WB Human
Cicchetti F, Lacroix S, Cisbani G et al. Mutant huntingtin is present in neuronal grafts in Huntington's disease patients. Ann. Neurol. 2014 May 06 [PMID: 24798518] (IHC-Fr, Human) IHC-Fr Human
Tu C, FosterL, Alvarado A et al. Role of Zinc in Catalytic Activity of Carbonic Anhydrase IX Archives of Biochemistry and Biophysics 2012 Mar 12 [PMID: 22465027] (WB, Human) WB Human
Li Y, Tu C, Wang H et al. Catalysis and pH control by membrane-associated carbonic anhydrase IX in MDA-MB-231 breast cancer cells. J Biol Chem. 2011 Mar 17. [PMID: 21454639]
Shiah, H-S, Gao, W, Baker, D C, Cheng, Y-C. (2006) Inhibition of cell growth nuclear factor-{kappa}B activity in pancreatic cancer cell lines by a tylophorine analogue, DCB-3503, 5, 2484-2493. [PMID: 17041092]
Li X, Alvarez B, Casey JR et al. Carbonic anhydrase II binds to enhances activity of the Na+/H+ exchanger. J Biol Chem;277(39):36085-91. 2002 Sep 27. [PMID: 12138085]

Reviews for Carbonic Anhydrase II/CA2 Antibody (NB600-919) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB600-919:
Filter by Applications
Immunohistochemistry
(1)
Western Blot
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry Carbonic Anhydrase II/CA2 NB600-919
Enlarge
5
reviewed by:
Verified Customer
Immunohistochemistry Mouse 09/23/2014
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Summary

ApplicationImmunohistochemistry
Sample TestedMouse peritoneum
SpeciesMouse
Western Blot Carbonic Anhydrase II/CA2 NB600-919
Enlarge
5
reviewed by:
Verified Customer
Western Blot Mouse 06/24/2013
View

Summary

ApplicationWestern Blot
Sample TestedEmbryonic gestational day 14.5 mouse whole embryo lysates
SpeciesMouse
Lot13915
  5
reviewed by:
Verified Customer
Western Blot Human 10/06/2011
View

Summary

ApplicationWestern Blot
Sample TestedDetection of CA11 in human cancer cells
SpeciesHuman

Product General Protocols

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FAQs for Carbonic Anhydrase II/CA2 Antibody (NB600-919). (Showing 1 - 1 of 1 FAQs).

  1. I am going to perform CA2 / Carbonic Anhydrase II immunohistochemistry staining for bone samples. Is this antibody better for plastic embedded bone samples? Or does it only work on paraffin embedded samples or soft tissue?
    • NB600-919 has only been tested on paraffin-embedded tissues. It will most likely work on your samples, but it has never been tested in this application specifically. From our catalog, this antibody is the best choice for your experiments.

Control Lysate(s)

Secondary Antibodies

 

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Verified Customer
09/23/2014
Application: Immunohistochemistry
Species: Mouse

Verified Customer
06/24/2013
Application: Western Blot
Species: Mouse

 
Verified Customer
10/06/2011
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol CA2