Carbonic Anhydrase II/CA2 Antibody

Images

 
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4C. ...read more
Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells ...read more
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - WB analysis of Carbonic Anhydrase II protein (loading amount: 50 ng) using rabbit anti-Carbonic Anhydrase II antibody (NB600-919) at a dilution of 1:1000, ...read more
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Analysis using the Biotin conjugate of NB600-919. Detection of Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic ...read more
Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Localization of CAII in mouse peritoneum. Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

Carbonic Anhydrase II/CA2 Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Carbonic Anhydrase II Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II.
Localization
Cytoplasmic
Specificity
Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Gene
CA2
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
20mM Sodium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:165000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24798518)
Reviewed Applications
Read 3 Reviews rated 5
using
NB600-919 in the following applications:

Publications
Read Publications using
NB600-919 in the following applications:

Alternate Names for Carbonic Anhydrase II/CA2 Antibody

  • CA2
  • CAII
  • CA-IIEC 4.2.1.1
  • Car2
  • Carbonate dehydratase II
  • carbonic anhydrase 2
  • carbonic anhydrase B
  • Carbonic anhydrase C
  • Carbonic Anhydrase II
  • carbonic anhydrase IICAC
  • carbonic dehydratase

Background

Carbonic Anhydrase II Antibody detects Carbonic Anhydrase II. Carbonic Anhydrase II is essential for bone resorption and osteoclast differentiation by similarity. It can hydrate cyanamide to urea and is involved in the regulation of fluid secretion into the anterior chamber of the eye. Anti-Carbonic Anhydrase II Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2180
Species: Hu
Applications: WB, Simple Western, IP
AF2185
Species: Hu, Mu, Rt
Applications: WB, IP
AF2186
Species: Hu
Applications: WB, Simple Western, IHC, IP
NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF1936
Species: Hu
Applications: WB, IP
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye, Am(-), Av(-), Pm(-)
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP2-45897
Species: Hu
Applications: WB, IHC
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NB120-11012
Species: Hu, Bv(-), Ca(-), Eq(-), Gp(-), Mu(-), Po(-)
Applications: WB, ELISA, ICC/IF, IP
NBP1-86616
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56508
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P, Flow-CS
NB100-56505
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Carbonic Anhydrase II/CA2 Antibody (NB600-919)(5)

Reviews for Carbonic Anhydrase II/CA2 Antibody (NB600-919) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 2 application: IHC, WB.

Reviews using NB600-919:
Filter by Applications
IHC
(1)
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry Carbonic Anhydrase II/CA2 NB600-919
Enlarge
reviewed by:
Amadou Sow
IHC Mouse 09/23/2014
View

Summary

ApplicationImmunohistochemistry
Sample TestedMouse peritoneum
SpeciesMouse

Blocking

Blocking Details30 min with normal goat serum, RT

Primary Anitbody

Dilution Ratio1:200, 1 hour incubation time, RT, PBS-BSA 2%

Details

Detection NotesAvidin-Biotin
Fixation DetailsFormalin fixed, paraffin embedded, Citrate buffer Ag retrieval
Wash DescriptionPBS 1X, 3 times by step (5min)
Western Blot Carbonic Anhydrase II/CA2 NB600-919
Enlarge
reviewed by:
Corey.
WB Mouse 06/24/2013
View

Summary

ApplicationWestern Blot
Sample TestedEmbryonic gestational day 14.5 mouse whole embryo lysates
SpeciesMouse
Lot13915
CommentsThe antibody seemed to detect our protein at the proper molecular weight very nicely. Controls: Lanes 4, 5, 6 were WT and hemzygous for LCMT-1

Blocking

Blocking Details3% non-fat dry milk (NFDM) in 1xTBST for 1 hour

Primary Anitbody

Dilution RatioDiluted in 0.5% NFDM in 1xTBST at a dilution of 1:10,000, Overnight at 4C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit
Secondary Manufacturer Cat#JacksonImmunoResearch, cat. # 111-035-144
Secondary Concentrationdiluted in 0.5% NFDM in 1xTBST at a dilution of 1:5000

Details

Detection NotesWestern Lightning ECL Pro (PerkinEmler)

Comments

CommentsThe antibody seemed to detect our protein at the proper molecular weight very nicely. Controls: Lanes 4, 5, 6 were WT and hemzygous for LCMT-1
 
reviewed by:
Susan Frost
WB Human 10/06/2011
View

Summary

ApplicationWestern Blot
Sample TestedDetection of CA11 in human cancer cells
SpeciesHuman
CommentsVery strong affinity. Excellent Ab. Strong specificity.

Comments

CommentsVery strong affinity. Excellent Ab. Strong specificity.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Carbonic Anhydrase II/CA2 Antibody (NB600-919). (Showing 1 - 1 of 1 FAQs).

  1. I am going to perform CA2 / Carbonic Anhydrase II immunohistochemistry staining for bone samples. Is this antibody better for plastic embedded bone samples? Or does it only work on paraffin embedded samples or soft tissue?
    • NB600-919 has only been tested on paraffin-embedded tissues. It will most likely work on your samples, but it has never been tested in this application specifically. From our catalog, this antibody is the best choice for your experiments.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Carbonic Anhydrase II/CA2 Antibody Products

Related Products by Gene

Bioinformatics Tool for Carbonic Anhydrase II/CA2 Antibody (NB600-919)

Discover related pathways, diseases and genes to Carbonic Anhydrase II/CA2 Antibody (NB600-919). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Carbonic Anhydrase II/CA2 Antibody (NB600-919)

Discover more about diseases related to Carbonic Anhydrase II/CA2 Antibody (NB600-919).
 

Pathways for Carbonic Anhydrase II/CA2 Antibody (NB600-919)

View related products by pathway.

PTMs for Carbonic Anhydrase II/CA2 Antibody (NB600-919)

Learn more about PTMs related to Carbonic Anhydrase II/CA2 Antibody (NB600-919).
 

Research Areas for Carbonic Anhydrase II/CA2 Antibody (NB600-919)

Find related products by research area.

Blogs on Carbonic Anhydrase II/CA2

There are no specific blogs for Carbonic Anhydrase II/CA2, but you can read our latest blog posts.

Contact Information

Product PDFs

Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Amadou Sow
09/23/2014
Application: IHC
Species: Mouse

Corey.
06/24/2013
Application: WB
Species: Mouse

 
Susan Frost
10/06/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol CA2
Entrez
Uniprot

Customers Who Bought This Also Bought