Annexin A4 Antibody

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of ...read more
Annexin A4 was detected in immersion fixed paraffin-embedded sections of human kidney using Human/Mouse/Rat Annexin A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4146) at 10 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Annexin A4 Antibody Summary

Immunogen
E. coli-derived recombinant human Annexin A4
Met1-Asp321
Accession # NP_001144
Specificity
Detects endogenous human, mouse, and rat Annexin A4 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human Annexin A1, A2, A3, A5, A6, A7, A8, A9, A10, A11or A13.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANXA4
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4146 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin A4 Antibody

  • AIV
  • Annexin A4
  • annexin IV (placental anticoagulant protein II)
  • Annexin IV
  • annexin-4
  • ANX4
  • ANX4Carbohydrate-binding protein p33/p41
  • ANXA4
  • Chromobindin-4
  • chromobindin-4,35-beta calcimedin
  • DKFZp686H02120
  • Endonexin I
  • Lipocortin IV
  • MGC75105
  • P32.5
  • PAP-II
  • PIG28
  • Placental anticoagulant protein II
  • PP4-X
  • proliferation-inducing gene 28
  • proliferation-inducing protein 28
  • Protein II
  • ZAP36

Background

Annexin A4 (ANXA4), also known as Lipocortin IV, placental anticoagulant protein II and zymogen granule membrane-associated protein, 36 kDa (ZAP36), is a 321 amino acid, 36 kDa member of the Annexin protein family. Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C terminal domain is comprised of four 60‑70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Human Annexin A4 shares 92% identity with mouse and rat Annexin A4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A4 Antibody (AF4146)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IHC, IHC-Fr.


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(1)
IHC
(1)
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(1)
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Video Protocols

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Bioinformatics

Gene Symbol ANXA4
Uniprot