PEX3 Antibody - BSA Free

Western Blot: PEX3 Antibody - BSA Free [NBP3-18138] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: PEX3 NBP3-18138, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Western Blot: PEX3 Antibody - BSA Free [NBP3-18138] - WB Validation in Human Cell Lines. Loading: 15 ug of lysate Antibodies: PEX3 NBP3-18138, 2 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.

Western Blot: PEX3 Antibody - BSA Free [NBP3-18138] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: PEX3 NBP3-18138, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunohistochemistry: PEX3 Antibody - BSA Free [NBP3-18138] - Validation of PEX3 in Rat testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry: PEX3 Antibody - BSA Free [NBP3-18138] - Figure 6 Immunofluorescence Validation of PEX3 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry: PEX3 Antibody - BSA Free [NBP3-18138] - Figure 5 Immunofluorescence Validation of PEX3 in Human SpleenImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: PEX3 Antibody - BSA Free [NBP3-18138] - Validation of PEX3 in Human HeLa Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PEX3 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 12 amino acids near the carboxyl terminal of human PEX3. The immunogen is located within last 50 amino acids of PEX3.
• Predicted Species: Bovine (100%), Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PEX3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry
  • Western Blot 2 ug/mL
• Control Peptide:

  PEX3 Protein (NBP3-18138PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PEX3 Antibody - BSA Free

• DKFZp686N14184
• FLJ13531
• peroxin-3
• Peroxisomal assembly protein PEX3
• peroxisomal biogenesis factor 3
• transformation-related protein 18
• TRG18

Background

The product of the PEX3 gene is involved in peroxisome biosynthesis and integrity. It assembles membrane vesicles before the matrix proteins are translocated. Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause Zellweger syndrome (ZWS). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEX3 Antibody (NBP3-18138)(1)

Publication using NBP3-18138

• Oh J, Kim DK, Ahn SH, Kim HM et Al. A dual role of the conserved PEX19 helix in safeguarding peroxisomal membrane proteins iScience 2024-04-08 [PMID: 38585659]

Bioinformatics

• Gene Symbol: PEX3