ASH1 Antibody

Images

 
Western Blot: ASH1 Antibody [NB100-93290] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH1 antibody used for WB at 0.1 ug/ml. ...read more
Immunohistochemistry-Paraffin: ASH1 Antibody [NB100-93290] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: ASH1 Antibody [NB100-93290] - Detection of Human ASH1 on HeLa whole cell lysate using NB100-93290. ASH1 was also immunoprecipitated by rabbit anti-ASH1 antibody NB100-93289.
Western Blot: ASH1 Antibody [NB100-93290] - Whole cell lysate (50 ug) from mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiASH1 antibody used for WB at 0.4 ug/ml. Detection: ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ASH1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2919 and 2969 of human absent, small and homeotic disks protein 1 using the numbering given in entry CAI12722.1 (GeneID 55870).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASH1L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
ASH1 Knockout HeLa Cell Lysate
Publications
Read Publication using
NB100-93290 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASH1 Antibody

  • Absent small and homeotic disks protein 1 homolog
  • ash1 (absent, small, or homeotic)-like (Drosophila)
  • ASH1
  • ASH1L1
  • ASH1-like protein
  • EC 2.1.1.43
  • FLJ10504
  • huASH1
  • KMT2HKIAA1420
  • Lysine N-methyltransferase 2H
  • probable histone-lysine N-methyltransferase ASH1L

Background

This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASH1 Antibody (NB100-93290)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Mouse
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ASH1 Antibody (NB100-93290) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-93290AF350
Alexa Fluor 405 NB100-93290AF405
Alexa Fluor 488 NB100-93290AF488
Alexa Fluor 532 NB100-93290AF532
Alexa Fluor 594 NB100-93290AF594
Alexa Fluor 647 NB100-93290AF647
Alexa Fluor 700 NB100-93290AF700
Alexa Fluor 750 NB100-93290AF750
Biotin NB100-93290B
DyLight 350 NB100-93290UV
DyLight 405 NB100-93290V
DyLight 488 NB100-93290G
DyLight 550 NB100-93290R
DyLight 594 NB100-93290DL594
DyLight 650 NB100-93290C
DyLight 680 NB100-93290FR
DyLight 755 NB100-93290IR
FITC NB100-93290F
HRP NB100-93290H
Janelia Fluor 549 NB100-93290JF549
Janelia Fluor 646 NB100-93290JF646

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Contact Information

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Bioinformatics

Gene Symbol ASH1L
Entrez
Uniprot