ASH1 Antibody

Western Blot: ASH1 Antibody [NB100-93290] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds

Immunohistochemistry-Paraffin: ASH1 Antibody [NB100-93290] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: ASH1 Antibody [NB100-93290] - Detection of Human ASH1 on HeLa whole cell lysate using NB100-93290. ASH1 was also immunoprecipitated by rabbit anti-ASH1 antibody NB100-93289.

Western Blot: ASH1 Antibody [NB100-93290] - Whole cell lysate (50 ug) from mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiASH1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

ASH1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2919 and 2969 of human absent, small and homeotic disks protein 1 using the numbering given in entry CAI12722.1 (GeneID 55870).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASH1L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ASH1 Antibody

• Absent small and homeotic disks protein 1 homolog
• ash1 (absent, small, or homeotic)-like (Drosophila)
• ASH1
• ASH1L1
• ASH1-like protein
• EC 2.1.1.43
• FLJ10504
• huASH1
• KMT2HKIAA1420
• Lysine N-methyltransferase 2H
• probable histone-lysine N-methyltransferase ASH1L

Background

This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASH1 Antibody (NB100-93290)(1)

Publication using NB100-93290

• Liu S, Tian M, He F et al. Mutations in ASH1L confer susceptibility to Tourette syndrome Mol. Psychiatry 2019-10-31 [PMID: 31673123] (WB, Mouse)

Bioinformatics

• Gene Symbol: ASH1L
• Entrez:
  • 55870(Human)
• Uniprot:
  • CAI12722.1(Human)