PEX19 Antibody

Western Blot: PEX19 Antibody [NBP1-32925] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PEX19 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: PEX19 Antibody [NBP1-32925] - Methanol-fixed HeLa antibody (Green) at 1:500 dilution.

Immunohistochemistry-Paraffin: PEX19 Antibody [NBP1-32925] - Rat liver. PEX19 stained by PEX19 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: PEX19 Antibody [NBP1-32925] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:10000.

Western Blot: PEX19 Antibody [NBP1-32925] - A. 30 ug PC-12 whole cell lysate/extract, B. 30 ug Rat-2 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.

Western Blot: PEX19 Antibody [NBP1-32925] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PEX19 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: PEX19 Antibody [NBP1-32925] - Mouse kidney. PEX19 stained by PEX19 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PEX19 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PEX19. The exact sequence is proprietary.
• Localization: Cytoplasm, Peroxisome membrane, Lipid-anchor, Cytoplasmic side
• Predicted Species: Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PEX19
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for PEX19 Antibody

• D1S2223Ehousekeeping gene, 33kD
• HK3333 kDa housekeeping protein
• Peroxin-19
• peroxisomal biogenesis factor 19
• Peroxisomal farnesylated proteinFLJ55296
• PMP1
• PMPI
• PXFperoxin-19
• PXMP1

Background

This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause Zellweger syndrome (ZWS). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PEX19
• OMIM:
  • 214100(Human)
• Uniprot:
  • P40855(Human)
  • Q8VCI5(Mouse)