PEX19 Antibody

Images

 
Western Blot: PEX19 Antibody [NBP1-32925] - Sample (30 ug of whole cell lysate) A: Raji B: K562 C: THP-1 D: NCI-H929 12% SDS PAGE; antibody diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: PEX19 Antibody [NBP1-32925] - PEX19 was stained in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were stained against Dylight 550 (Red) ...read more
Immunohistochemistry-Paraffin: PEX19 Antibody [NBP1-32925] - Paraffin-embedded mouse testis. PEX19 antibody dilution: 1:500.
Western Blot: PEX19 Antibody [NBP1-32925] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:10000.
Western Blot: PEX19 Antibody [NBP1-32925] - A. 30 ug PC-12 whole cell lysate/extract, B. 30 ug Rat-2 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Immunocytochemistry/ Immunofluorescence: PEX19 Antibody [NBP1-32925] - Methanol-fixed HeLa antibody (Green) at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PEX19 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PEX19. The exact sequence is proprietary.
Localization
Cytoplasm, Peroxisome membrane, Lipid-anchor, Cytoplasmic side
Predicted Species
Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PEX19
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
Useful for ICC/IF, IHC-P, and WB. WB: Predicted MW is 33 kDa.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PEX19 Antibody

  • D1S2223Ehousekeeping gene, 33kD
  • HK3333 kDa housekeeping protein
  • Peroxin-19
  • peroxisomal biogenesis factor 19
  • Peroxisomal farnesylated proteinFLJ55296
  • PMP1
  • PMPI
  • PXFperoxin-19
  • PXMP1

Background

This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause Zellweger syndrome (ZWS). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PEX19
OMIM
Uniprot