Peripherin Antibody (7C5)

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Immunocytochemistry/ Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, MaSpecies Glossary
Applications WB, ICC/IF
Clone
7C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Peripherin Antibody (7C5) Summary

Immunogen
Recombinant rat Peripherin purified from E. coli. [UniProt# P21807]
Marker
Un-myelinated Neurons Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PRPH
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This Peripherin (7C5) antibody can be used in Immunocytochemistry/Immunofluorescence and Western blot. A band at ~57 kDa can be seen in Western blot. This clone is relatively insensitive to aldehyde fixation, making it superior to clone 8G2 for this purpose. *The investigator should determine the optimal dilution for a specific application. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken. Expected to cross-react with all mammals due to sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
5mM Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Peripherin Antibody (7C5)

  • neurofilament 4 (57kD)
  • Neurofilament 4
  • peripherin
  • PRPH1NEF4

Background

Peripherin (PRPH) is a 57kD type III neuronal intermediate filament (NIF) protein which is predominantly expressed in neurons of PNS and in certain CNS neurons specifically from brain stem/spinal cord which generally project toward peripheral structures. Inside developing neurons, PRPH is expressed abundantly in the growth cones and is required for axonal outgrowth as well as maintenance or regeneration. In mature neurons, together with other NIFs, PRPH constitutes a shape-maintaining IF network. Besides its crucial role in development of nervous system, PRPH can also be highly relevant to pathogenesis of neurodegenerative disorder. PRPH expression is induced in CNS neurons (wherein it is not expressed normally) upon brain injury, and factors such as LIF, IL-6 etc and PRPH molecules form aggregates in neurons. Synaptic plasticity is markedly altered in PRPH transgenic mice (Per mice) and up-regulation of PRPH expression is implicated in some pathophysiological conditions, such as cerebral ischemia and seizure, and some cognitive disorders. In ALS patients, PRPH together with neuronal IFs are detected in the majority of abnormal IF inclusion bodies in mature or aging motor neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Peripherin Antibody (NB300-220) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Peripherin Antibody (NB300-220)

Discover related pathways, diseases and genes to Peripherin Antibody (NB300-220). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Peripherin Antibody (NB300-220)

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Pathways for Peripherin Antibody (NB300-220)

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PTMs for Peripherin Antibody (NB300-220)

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Blogs on Peripherin.

A Look at Peripherin: The Unknown Filament
The exact function of Peripherin, or Neurofilament 4, is unknown however it has been suggested to play a role in axon formation and determining and maintaining the shape of nerve cells. Peripherin is a 470 amino acid Class-III neuronal intermediate fi...  Read full blog post.

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Bioinformatics

Gene Symbol PRPH