Peripherin Antibody (7C5) - BSA Free

Western Blot: Peripherin Antibody (7C5) [NB300-220] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:1,000 (Green): [1] protein standard, [2] rat spinal cord, [3] rat sciatic nerve, [4] mouse spinal cord, [5] pig spinal cord, [6] cow spinal cord, [7] SH-SY5Y cells, and [8] PC12 cells. The band at ~57kDa corresponds to the peripherin protein detected only in rat and mouse lysates, since the antibody is rodent specific.

Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Analysis of mixed fibroblast and rat pheochromocytoma PC12 cell culture stained with peripherin antibody, dilution 1:500 (Red), and costained with chicken pAb to vimentin, dilution 1:10,000 (Green). Hoechst staining of nuclear DNA (Blue). The peripherin antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.

Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L NBP1-05218 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.

• Reactivity: Mu, Rt, Hu(-)
• Applications: WB, ICC/IF, IHC
• Clone: 7C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.

Peripherin Antibody (7C5) - BSA Free Summary

• Description: Novus Biologicals Mouse Peripherin Antibody (7C5) - BSA Free (NB300-220) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Peripherin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant rat Peripherin purified from E. coli. [UniProt# P21807]
• Marker: Un-myelinated Neurons Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRPH
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Western Blot 1:1000
• Application Notes: This Peripherin (7C5) antibody can be used in Immunocytochemistry/Immunofluorescence and Western blot. A band at ~57 kDa can be seen in Western blot. This clone is relatively insensitive to aldehyde fixation, making it superior to clone 8G2 for this purpose. *The investigator should determine the optimal dilution for a specific application.
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Tissue culture supernatant

Alternate Names for Peripherin Antibody (7C5) - BSA Free

• neurofilament 4 (57kD)
• Neurofilament 4
• peripherin
• PRPH1NEF4

Background

Peripherin (PRPH) is a 57kD type III neuronal intermediate filament (NIF) protein which is predominantly expressed in neurons of PNS and in certain CNS neurons specifically from brain stem/spinal cord which generally project toward peripheral structures. Inside developing neurons, PRPH is expressed abundantly in the growth cones and is required for axonal outgrowth as well as maintenance or regeneration. In mature neurons, together with other NIFs, PRPH constitutes a shape-maintaining IF network. Besides its crucial role in development of nervous system, PRPH can also be highly relevant to pathogenesis of neurodegenerative disorder. PRPH expression is induced in CNS neurons (wherein it is not expressed normally) upon brain injury, and factors such as LIF, IL-6 etc and PRPH molecules form aggregates in neurons. Synaptic plasticity is markedly altered in PRPH transgenic mice (Per mice) and up-regulation of PRPH expression is implicated in some pathophysiological conditions, such as cerebral ischemia and seizure, and some cognitive disorders. In ALS patients, PRPH together with neuronal IFs are detected in the majority of abnormal IF inclusion bodies in mature or aging motor neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peripherin Antibody (NB300-220)(1)

Publication using NB300-220

• Becherel OJ, Yeo AJ, Stellati A et al. Senataxin Plays an Essential Role with DNA Damage Response Proteins in Meiotic Recombination and Gene Silencing. PLoS Genet 2013-04-01 [PMID: 23593030]

Bioinformatics

• Gene Symbol: PRPH