NF-L Antibody (DA2)

Images

 
Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with ...read more
Immunocytochemistry/ Immunofluorescence: NF-L Antibody (DA2) [NB300-132] - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) taken with a 40X lens. Nuclei are stained blue. Photo Courtesy of Dr. ...read more
Simple Western: NF-L Antibody (DA2) [NB300-132] - Simple Western lane view shows a specific band for NF-L in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Av, MaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clone
DA2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NF-L Antibody (DA2) Summary

Immunogen
Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]
Epitope
The epitope for this antibody is not in the N-terminus; however exactly where it is located is not known. It is thought to be somewhere within the central alpha helical rod region of the molecule.
Localization
Cytoplasmic
Marker
Neuronal Marker
Specificity
Specifically recognizes the light neurofilament subunit or NF-L (~68 kDa).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NEFL
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:10000
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin
Application Notes
This 68kDa Neurofilament Light antibody is useful in Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25565998).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
NF-L Lysate (NBL1-13577)
Publications
Read Publications using
NB300-132 in the following applications:

Reactivity Notes

Human and other mammals. Also reacts with avian.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for NF-L Antibody (DA2)

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NF-L Antibody (NB300-132)(4)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(2)
WB
(1)
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Filter By Species
Rat
(3)
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Showing Publications 1 - 4 of 4.
Publications using NB300-132 Applications Species
Aboelenain M, Kawahara M, Balboula AZ et al. Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle. J Reprod Dev 2015 Jun 20 [PMID: 25819401]
Altabella L, Sbriccoli M, Zoratto F et al. Differential responses to acute administration of a new 5-HT7-R agonist as a function of adolescent pre-treatment: phMRI and immuno-histochemical study. Front Behav Neurosci. 2015 Jan 08 [PMID: 25565998] (IHC-P, Rat) IHC-P Rat
King AE, Dickson TC, Blizzard CA et al. Excitotoxicity mediated by non-NMDA receptors causes distal axonopathy in long-term cultured spinal motor neurons. Eur J Neurosci. 2007 Oct [PMID: 17908171] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Altabella L, Sbriccoli M, Zoratto F. Differential responses to acute administration of a new 5-HT7-R agonist asa function of adolescent pre-treatment: phMRI and immuno-histochemicalstudy name: Frontiers in behavioral neuroscience et al. 2014 (IHC-P, Rat)

Details:
nF-L antibody used at 1:250 dilution for IHC-P on sections of brain hemispheres from Rats subjected to acute administration of LP-211 [selective agonist of serotonin (5-hydroxytryptamine, 5-HT) receptor 7 (5-HT7-R)], or vehicle.
IHC-P Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-L Antibody (NB300-132). (Showing 1 - 1 of 1 FAQs).

  1. Can you please tell me if the 68 kDa NF-L antibody (DA2) epitope is in the tail domain or the rod domain of the protein?
    • The epitope for this antibody is not in the N-terminus; however, exactly where it is located is not known. It is thought to be somewhere within the central alpha helical rod region of the molecule.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEFL
Entrez