NF-L Antibody (DA2) - BSA Free

Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).

Immunocytochemistry/Immunofluorescence: NF-L Antibody (DA2) [NB300-132] - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) imaged with a 40X lens. Nuclei are stained blue. Photo Courtesy of Dr. Gerry Shaw.

Simple Western: NF-L Antibody (DA2) [NB300-132] - Image shows a specific band for NF-L in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Av, Bv, Ma
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: DA2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

NF-L Antibody (DA2) - BSA Free Summary

• Immunogen: Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]
• Epitope: The epitope for this antibody is not in the N-terminus; however exactly where it is located is not known. It is thought to be somewhere within the central alpha helical rod region of the molecule.
• Localization: Cytoplasmic
• Marker: Neuronal Marker
• Specificity: Specifically recognizes the light neurofilament subunit or NF-L (~68 kDa).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEFL
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 25565998)
  • Simple Western 1:50
  • Western Blot 1:5000 - 1:10000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 81 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  NF-L Overexpression Lysate

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25819401).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for NF-L Antibody (DA2) - BSA Free

• 68 kDa neurofilament protein
• CMT2E
• NEFL
• neurofilament protein, light chain
• neurofilament subunit NF-L
• Neurofilament triplet L protein
• neurofilament, light polypeptide
• neurofilament-light
• NF68
• NF68FLJ53642
• NFL
• NF-L
• NFLlight polypeptide 68kDa

Background

Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-L Antibody (NB300-132)(5)

Publications using NB300-132

• Aboelenain M, Kawahara M, Balboula AZ et al. Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle. J Reprod Dev 2015-06-20 [PMID: 25819401]
• Altabella L, Sbriccoli M, Zoratto F. Differential responses to acute administration of a new 5-HT7-R agonist asa function of adolescent pre-treatment: phMRI and immuno-histochemicalstudy name: Frontiers in behavioral neuroscience et al. 2014-01-01 [PMID: 25565998] (IHC-P, Rat)
• Altabella L, Sbriccoli M, Zoratto F et al. Differential responses to acute administration of a new 5-HT7-R agonist as a function of adolescent pre-treatment: phMRI and immuno-histochemical study. Front Behav Neurosci. 2015-01-08 [PMID: 25565998] (IHC-P, Rat)
• King AE, Dickson TC, Blizzard CA et al. Excitotoxicity mediated by non-NMDA receptors causes distal axonopathy in long-term cultured spinal motor neurons. Eur J Neurosci. 2007-10-01 [PMID: 17908171] (ICC/IF, WB, Rat)
• Xu Z, Shang J Neoneurogenesis in Squamous Cell Carcinoma of Tongue: A New Mechanism for Its Development OJST 2019-05-13 (IHC-P, Human)

FAQs for NF-L Antibody (NB300-132). (Showing 1 - 1 of 1 FAQs).

1. Can you please tell me if the 68 kDa NF-L antibody (DA2) epitope is in the tail domain or the rod domain of the protein?
   • The epitope for this antibody is not in the N-terminus; however, exactly where it is located is not known. It is thought to be somewhere within the central alpha helical rod region of the molecule.

Bioinformatics

• Gene Symbol: NEFL
• Entrez:
  • 4747(Human)