Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with ...read more
Immunocytochemistry/ Immunofluorescence: NF-L Antibody (DA2) [NB300-132] - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) taken with a 40X lens. Nuclei are stained blue. Photo Courtesy of Dr. ...read more
Simple Western: NF-L Antibody (DA2) [NB300-132] - Simple Western lane view shows a specific band for NF-L in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
This 68kDa Neurofilament Light antibody is useful in Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25565998).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Alternate Names for NF-L Antibody (DA2)
68 kDa neurofilament protein
neurofilament protein, light chain
neurofilament subunit NF-L
Neurofilament triplet L protein
neurofilament, light polypeptide
NFLlight polypeptide 68kDa
Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
We have publications tested in 1 confirmed species: Rat.
We have publications tested in 3 applications: ICC/IF, IHC-P, WB.
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Showing Publications 1 - 4 of 4.
Publications using NB300-132
Altabella L, Sbriccoli M, Zoratto F. Differential responses to acute administration of a new 5-HT7-R agonist asa function of adolescent pre-treatment: phMRI and immuno-histochemicalstudy name: Frontiers in behavioral neuroscience et al. 2014 (IHC-P, Rat)
Details: nF-L antibody used at 1:250 dilution for IHC-P on sections of brain hemispheres from Rats subjected to acute administration of LP-211 [selective agonist of serotonin (5-hydroxytryptamine, 5-HT) receptor 7 (5-HT7-R)], or vehicle.
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