Periostin/OSF-2 Antibody - Azide and BSA Free

Western Blot: Periostin/OSF-2 - Western blot of rat lung lysate showing specific immunolabeling of the ~93 kDa Periostin/OSF-2 protein triplet.

• Reactivity: Hu, Mu, Rt, Ch, Am, Av, Bv, Ca, Eq, Fe, Av-Bi, Fi, Gt, Gp, Ha, Pm, Rb, Sh, Vo, Xp, Ze
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Periostin/OSF-2 Antibody - Azide and BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Synthetic peptide corresponding to amino acid residues from the fasciclin domain 1 of Periostin/OSF-2. Accession # Q62009
• Specificity: Specific for endogenous levels of the three Periostin/OSF-2 bands around ~93 kDa. The immunogenic peptide is present in all known splice variants of Periostin/OSF-2 and therefore this antibody recognizes all known molecular forms of Periostin/OSF-2 and is thus pan-specific.
• Predicted Species: Hamster (100%), Canine (100%), Goat (100%), Sheep (100%), Equine (100%), Vole (100%), Xenopus (100%), Zebrafish (100%), Feline (100%), Bovine (100%), Guinea Pig (100%), Rabbit (100%), Finch (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POSTN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100
  • Western Blot 1:1000
• Application Notes: This Periostin/OSF-2 Antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20)
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected reactivity with Avian, Amphibians, Fish, and most mammals.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Periostin/OSF-2 Antibody - Azide and BSA Free

• Fasciclin I-like
• MGC119510
• MGC119511
• OSF2
• OSF-2
• OSF-2osteoblast specific factor 2 (fasciclin I-like)
• OSF2periodontal ligament-specific periostin
• Osteoblast-specific factor 2
• PDLPOSTN
• periostin isoform thy2
• periostin isoform thy4
• periostin isoform thy6
• periostin isoform thy8
• Periostin
• periostin, osteoblast specific factor
• PNRP11-412K4.1
• POSTN
• TRIF52

Background

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POSTN