Periostin/OSF-2 Antibody (345613) [Unconjugated]

Periostin/OSF-2 was detected in immersion fixed human osteocytes using Rat Anti-Human/Mouse Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB3548) at 10 µg/mL for 3 hours at room temperature. Cells were stained red and counterstained with DAPI (blue).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 345613
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Periostin/OSF-2 Antibody (345613) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin isoform 2
  Asn24-Gln811
  Accession # NP_056599
• Specificity: Detects mouse Periostin/OSF-2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: POSTN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Periostin/OSF-2 Antibody (345613) [Unconjugated]

• Fasciclin I-like
• MGC119510
• MGC119511
• OSF2
• OSF-2
• OSF-2osteoblast specific factor 2 (fasciclin I-like)
• OSF2periodontal ligament-specific periostin
• Osteoblast-specific factor 2
• PDLPOSTN
• periostin isoform thy2
• periostin isoform thy4
• periostin isoform thy6
• periostin isoform thy8
• Periostin
• periostin, osteoblast specific factor
• PNRP11-412K4.1
• POSTN
• TRIF52

Background

Mouse periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1-4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while theC-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672-812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787, and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha _v beta ₃ and alpha _v beta ₅ integrins (3). It is synthesized by smooth muscle cells, fibroblasts, and osteoblasts (2, 5, 7). Mature mouse OSF-2 is 98%, 92%, and 91% aa identical to rat, canine, and human OSF-2, respectively.

1. Clout, N.J. and D. Tisi (2003) Structure 11:197.
2. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
3. Gillan, L. et al. (2002) Cancer Res. 62:5358.
4. Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
6. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
7. Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
8. Takeshita, S. et al. (1993) Biochem. J. 294:271.
9. Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
10. Swiss-Prot Accession #:Q62009.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Periostin/OSF-2 Antibody (MAB3548)(11)

Publications using MAB3548

• Calyeca, J;Hussein, Z;Tan, ZH;Liu, L;Dharmadhikari, S;Shontz, KM;Vetter, TA;Breuer, CK;Reynolds, SD;Chiang, T; Orchestrated response from heterogenous fibroblast subsets contributes to repair from surgery-induced stress after airway reconstruction JCI insight 2025-01-21 [PMID: 39836476] (Immunohistochemistry, Mouse)
• Cho, HH;Rhee, S;Cho, DI;Jun, JH;Heo, H;Cho, SH;Kim, D;Wang, M;Kang, BG;Yoo, SJ;Cho, M;Lim, SY;Cho, JY;Jeong, IS;Kim, YS;Ahn, Y; IKK?-deficient macrophages impede cardiac repair after myocardial infarction by enhancing the macrophage-myofibroblast transition Experimental & molecular medicine 2024-09-01 [PMID: 39261656] (Immunohistochemistry, Human)
• Vincent R. Knight-Schrijver, Hongorzul Davaapil, Semih Bayraktar, Alexander D. B. Ross, Kazumasa Kanemaru, James Cranley et al. A single-cell comparison of adult and fetal human epicardium defines the age-associated changes in epicardial activity Nature Cardiovascular Research 2022-12-21 [PMID: 36938497]
• CE Dorrier, D Aran, EA Haenelt, RN Sheehy, KK Hoi, L Pintari?, Y Chen, CO Lizama, KM Cautivo, GA Weiner, B Popko, SPJ Fancy, TD Arnold, R Daneman CNS fibroblasts form a fibrotic scar in response to immune cell infiltration Nature Neuroscience, 2021-02-01;24(2):234-244. 2021-02-01 [PMID: 33526922] (IHC, Mouse)
• Kinchen J, Chen HH, Parikh K et al. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell 9/25/2018 [PMID: 30270042]
• T Nakama, S Yoshida, K Ishikawa, Y Kubo, Y Kobayashi, Y Zhou, S Nakao, T Hisatomi, Y Ikeda, K Takao, K Yoshikawa, A Matsuda, J Ono, S Ohta, K Izuhara, A Kudo, KH Sonoda, T Ishibashi Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization Mol Ther Nucleic Acids, 2017-02-09;6(0):279-289. 2017-02-09 [PMID: 28325294] (IHC-P, Mouse)
• Yang , Lingli, Murota , Hiroyuki, Serada , Satoshi, Fujimoto , Minoru, Kudo , Akira, Naka , Tetsuji, Katayama , Ichiro Histamine contributes to tissue remodeling via periostin expression. J Invest Dermatol, 2014-02-27;134(8):2105-13. 2014-02-27 [PMID: 24577408] (Western Blot, Mouse)
• Beta3 integrin modulates transforming growth factor beta induced (TGFBI) function and paclitaxel response in ovarian cancer cells. Mol. Cancer, 2012-05-28;11(0):36. 2012-05-28 [PMID: 22640878] (Western Blot, Human)
• Kitajima N, Watanabe K, Morimoto S, Sato Y, Kiyonaka S, Hoshijima M, Ikeda Y, Nakaya M, Ide T, Mori Y, Kurose H, Nishida M TRPC3-mediated Ca2+ influx contributes to Rac1-mediated production of reactive oxygen species in MLP-deficient mouse hearts. Biochem. Biophys. Res. Commun., 2011-05-03;409(1):108-13. 2011-05-03 [PMID: 21565173] (Western Blot, Mouse)
• Daihiko Hakuno, Naritaka Kimura, Masatoyo Yoshioka, Makio Mukai, Tokuhiro Kimura, Yasunori Okada et al. Periostin advances atherosclerotic and rheumatic cardiac valve degeneration by inducing angiogenesis and MMP production in humans and rodents Journal of Clinical Investigation 2010-07-01 [PMID: 20551517]
• Stephens EH, Post AD, Laucirica DR, Grande-Allen KJ. Perinatal changes in mitral and aortic valve structure and composition. Pediatr Dev Pathol. 11/1/2010 [PMID: 20536360]

Bioinformatics

• Gene Symbol: POSTN
• Uniprot:
  • NP_056599()