Periostin/OSF-2 Antibody (345613) [Alexa Fluor™ Plus 680]

• Clone: 345613
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 680

Periostin/OSF-2 Antibody (345613) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects mouse Periostin/OSF-2 in direct ELISAs and Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Periostin/OSF-2 Antibody (345613) [Alexa Fluor™ Plus 680]

• Fasciclin I-like
• MGC119510
• MGC119511
• OSF2
• OSF-2
• OSF-2osteoblast specific factor 2 (fasciclin I-like)
• OSF2periodontal ligament-specific periostin
• Osteoblast-specific factor 2
• PDLPOSTN
• periostin isoform thy2
• periostin isoform thy4
• periostin isoform thy6
• periostin isoform thy8
• Periostin
• periostin, osteoblast specific factor
• PNRP11-412K4.1
• POSTN
• TRIF52

Background

Mouse periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1-4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while theC-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672-812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787, and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha _v beta ₃ and alpha _v beta ₅ integrins (3). It is synthesized by smooth muscle cells, fibroblasts, and osteoblasts (2, 5, 7). Mature mouse OSF-2 is 98%, 92%, and 91% aa identical to rat, canine, and human OSF-2, respectively.

1. Clout, N.J. and D. Tisi (2003) Structure 11:197.
2. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
3. Gillan, L. et al. (2002) Cancer Res. 62:5358.
4. Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
6. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
7. Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
8. Takeshita, S. et al. (1993) Biochem. J. 294:271.
9. Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
10. Swiss-Prot Accession #:Q62009.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.