Abhd5 Antibody (1F3)

Images

 
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody (M01), clone 1F3.Lane 1: ABHD5 transfected lysate(39 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Western Blot analysis of ABHD5 expression in Jurkat(Cat # L017V1 ).
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Western Blot analysis of ABHD5 expression in human liver.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in HeLa.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in rat testis.
ELISA: Abhd5 Antibody (1F3) [H00051099-M01] - Detection limit for recombinant GST tagged ABHD5 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, KO
Clone
1F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Abhd5 Antibody (1F3) Summary

Immunogen
ABHD5 (AAH21958, 1 a.a. ~ 349 a.a) full length recombinant protein with GST tag.MAAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSHNISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRCALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQ
Specificity
ABHD5 - abhydrolase domain containing 5
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ABHD5
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockout Validated
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Knock Out Validation was reported in scientific literature (PMID: 27559856).
Reviewed Applications
Read 1 Review rated 3
using
H00051099-M01 in the following applications:

Publications
Read Publications using
H00051099-M01 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • KO
    1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in multiple pieces of scientific literature

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Abhd5 Antibody (1F3)

  • abhydrolase domain containing 5
  • Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5
  • CDS
  • CGI58
  • EC 2.3.1.51
  • IECN2
  • Lipid droplet-binding protein CGI-58
  • MGC8731
  • NCIE2CGI-58

Background

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00051099-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, KO

Publications for Abhd5 Antibody (H00051099-M01)(20)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, KO, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
KO
(1)
WB
(5)
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Human
(1)
Mouse
(5)
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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using H00051099-M01 Applications Species
Bhavya S, Lew PS, Mizuno TM. Central action of xenin affects the expression of lipid metabolism-related genes and proteins in mouse white adipose tissue. Neuropeptides 2017 Feb 9 [PMID: 28190525]
Peng Y, Miao H, Wu S et al. ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2. Autophagy 2016 Nov [PMID: 27559856] (KO, Mouse) KO Mouse
Miao H, Ou J, Peng Y et al. Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM. Nat Commun 2016 May 18 [PMID: 27189574] (WB) WB
Vieyres G, Welsch K, Gerold G et al. ABHD5/CGI-58, the Chanarin-Dorfman Syndrome Protein, Mobilises Lipid Stores for Hepatitis C Virus Production. PLoS Pathog 2016 Apr [PMID: 27124600] (WB) WB
Heier C, Radner FP, Moustafa T et al. G0/G1 switch gene-2 regulates cardiac lipolysis. J Biol Chem. 2015 Sep 08 [PMID: 26350455]
Hofer P, Boeszoermenyi A, Jaeger D et al. Fatty acid-binding proteins interact with comparative gene identification-58 linking lipolysis with lipid ligand shuttling. J Biol Chem. 2015 May 07 [PMID: 25953897]
Das SK, Stadelmeyer E, Schauer S et al. Micro RNA-124a regulates lipolysis via adipose triglyceride lipase and comparative gene identification 58. Int J Mol Sci 2015 [PMID: 25894224] (ICC/IF) ICC/IF
Bakke SS, Feng YZ, Nikolic N et al. Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects. PLoS One 2015 [PMID: 25790476] (WB, Human) WB Human
Xie P, Kadegowda AK, Ma Y et al. Muscle-Specific Deletion of Comparative Gene Identification-58 (CGI-58) Causes Muscle Steatosis but Improves Insulin Sensitivity in Male Mice. Endocrinology. 2015 Mar 09 [PMID: 25751639] (WB, IHC, Mouse) WB, IHC Mouse
Camus G, Schweiger M, Herker E et al. The hepatitis C virus core protein inhibits adipose triglyceride lipase (ATGL)-mediated lipid mobilization and enhances the ATGL interaction with comparative gene identification 58 (CGI-58) and lipid droplets. J Biol Chem. 2014 Dec 26 [PMID: 25381252]
Show All 20 Publications.

Review for Abhd5 Antibody (H00051099-M01) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Bovine, Mouse, Human.
We have 1 review tested in 1 application: WB.

Reviews using H00051099-M01:
Filter by Applications
WB
(1)
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Filter by Species
Bovine, Mouse, Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Abhd5 H00051099-M01
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reviewed by:
Heather Talbott
WB Bovine, Mouse, Human 09/12/2017
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Summary

ApplicationWestern Blot
Sample Tested Hela whole cell lysate,Mouse adipose tissue,mouse liver lysate,bovine adipose tissue lysate,bovine liver tissue lysate,HeLa Cytosol
SpeciesBovine, Mouse, Human
LotH5091-1F3

Comments

Comments4-20% gradient gel
15 ug/well
PVDF and Nitocellulose
Block: 5% NFDM in TBS 0.1% Tween-20
Primary: 1:500 dilution, 1% NFDM in TBS 0.1% Tween-20, overnight at 4 C
Secondary: 1:10,000 anti-mouse - HRP (Jackson ImmunoResearch 115-035-205); 1:5000 anti-biotin - HRP (Cell Signaling 7075P5), 1% NFDM in TBS 0.1% Tween-20, 1 hr at RT
ECL: SuperSignal West Femto (ThermoFisher Scientific 34096), UVP Image Station


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Recent Reviews

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5
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4
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3
1
2
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1
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Heather Talbott
09/12/2017
Application: WB
Species: Bovine, Mouse, Human

Bioinformatics

Gene Symbol ABHD5
Entrez
OMIM
Uniprot