Perilipin Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ShSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Perilipin Antibody [FITC] Summary

Immunogen
A synthetic peptide made to a region between residues 450-522 (C-terminus) of the human Perilipin protein. [Swiss-Prot# O60240]
Localization
Endoplasmic reticulum, Lipid droplet (surface-associated).
Predicted Species
Bovine (92%), Sheep (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Perilipin antibody is useful for Western blot anlaysis where a band at ~60 kDa is seen. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Perilipin Antibody [FITC]

  • PERI
  • perilipin 1
  • Perilipin
  • perilipin-1
  • PLIN
  • PLIN1
  • PLINLipid droplet-associated protein

Background

Perilipin is a member of the perilipin or PAT family which includes perilipin or perilipin 1 (PLIN1), ADRP/perilipin 2, TIP47/perilipin 3, S3-12/perilipin 4, and LSDP5/perilipin 5 (also called OXPAT). PLIN1 is localized on lipid droplet surfaces in adipocytes and steroidogenic cells wherein it plays a key role in basal/stimulated lipid storage as well as breakdown. Under basal conditions, perilipin protects the lipid core from lipases (e.g. hormone-sensitive lipase and adipocyte TAG lipase) and co-activator CGI-58 by sequestering the enzymes from the lipid droplet surfaces. However, upon beta-adrenergic stimulation, PKA phosphorylates PLIN1 and causes the release of CGI-58 so it can bind and stimulate ATGL and also allows/recruits HSL to translocate to the lipid droplet surface. PLIN1 knockout increases basal lipolysis and decreases LD size in adipocytes and causes resistance to diet induced obesity in mice. In humans, lower PLIN1 expression is associated with higher rates of lipolysis and mutation in PLIN1 or its expression in obese human adipose tissue, correlates positively with insulin sensitivity, and have been established as the cause of familial partial lipodystrophy type 4 (FPLD4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Perilipin Antibody (NB110-40760F). (Showing 1 - 1 of 1 FAQ).

  1. Does this antibody conjugated with FITC work even for Immunofluorescence assay?
    • The FITC conjugate of this perilipin antibody, NB110-40760F, has not itself been tested in ICC/IF yet, which is why this application is not stated on its datasheet. Nonetheless, the unconjugated format of the antibody, NB110-40760, has, and so is guaranteed for ICC/IF. Our product notes for this antibody say that use in ICC/IF was reported in the literature, in this paper: http://www.ncbi.nlm.nih.gov/pubmed/18201980) Therefore, we cannot guarantee NB110-40760F for IF, but the evidence we do have does indicate that it should work for you. If you did go ahead and test NB110-40760F in IF, you would be eligible for our Innovator's Reward, where in return for sharing your findings with us (whether the antibody proved suitable or not) by completing a review, we would give you a voucher code for the price of the product (please see here for further details: Innovator's Reward program).

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Perilipin Antibody (NB110-40760F)

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Bioinformatics

Gene Symbol PLIN1
Entrez