PDZK1 Antibody

Images

 
Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.
Immunocytochemistry/ Immunofluorescence: PDZK1 Antibody [NB400-149] - PDZK1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Immunohistochemistry: PDZK1 Antibody [NB400-149] - IHC analysis of PDZK1 in mouse kidney using DAB with hematoxylin counterstain.
Simple Western: PDZK1 Antibody [NB400-149] - Simple Western lane view shows a specific band for PDZK1 in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Rt(-)Species Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PDZK1 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminus of human PDZK1. [UniProt# Q13113]
Localization
Cytoplasmic and associated with peripheral membranes; localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDZK1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:250
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This PDZK1 antibody is useful for Immunohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a single band is observed at approx. 70 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB400-149 in the following applications:

Reactivity Notes

Human, Bovine and mouse. Does not react with rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for PDZK1 Antibody

  • CAP70
  • CFTR-associated protein of 70 kDa
  • CLAMP
  • Na(+)/H(+) exchanger regulatory factor 3
  • Na/Pi cotransporter C-terminal-associated protein 1
  • NaPi-Cap1
  • NHERF3
  • NHERF-3
  • NHERF3PDZD1Na(+)/H(+) exchange regulatory cofactor NHE-RF3
  • PDZ domain containing 1
  • PDZ domain-containing protein 1
  • PDZ-containing kidney protein 1
  • PDZD1
  • PDZK1
  • Sodium-hydrogen exchanger regulatory factor 3

Background

Scaffold or adaptor proteins recruits and/or anchor their binding partners to specific subcellular locations, serving as a platform to regulate interactions between proteins involved in diverse signal transduction pathways, and PDZK1 (PDZ domain-containing protein 1) is a scaffold protein belonging to NHERFs family. PDZK1 expression is largely limited to epithelial cells and is mainly expressed in renal proximal epithelial cells, hepatocytes, and at a lower level in other epithelial as well as certain endothelial cells. PDZK1 has four PDZ domains which facilitates its interaction with its binding partners, including ion transporters (e.g. CFTR, OCTN1/OCTN2 etc) and several GPCRs (e.g. HTR2B, SSTR family members). Through its interaction with HDL and SR-BI, PDZK1 can play role in reverse cholesterol transport and for HDL-mediated vascular re-endothelialisation. When complex with SLC9A3R1, PDZK1 cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking as well as the activity of associated membrane proteins. PDZK1 plays a role in cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. PDZK1 also implicates in connecting SCARB1 with cellular machineries for intracellular cholesterol transport and/or metabolism and regulation of proximal tubular Na+-dependent inorganic phosphate cotransport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDZK1 Antibody (NB400-149)(3)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PDZK1
Entrez
Uniprot