SLC34A1 Antibody (10B1.3E9)

Images

 
Western Blot: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - WB detection of SLC34A1 /NPTIIa protein in a lysate of mouse thymus using SLC34A1 clone 10B1.3E9 at its 1:500 dilution. The antibody detected a single specific ...read more
Immunocytochemistry/ Immunofluorescence: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - IHC analysis of a formalin-fixed and paraffin-embedded tissue section of human normal kidney using SLC34A1 antibody (clone 10B1.3E9) at 1:75 ...read more
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216PCP (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - FLOW detection of SLC34A1 protein on HEK293 cells - After fixation and permeabilization, 2 x 10^6 cells/ml were stained using SLC34A1 antibody (clone 10B1.3E9) ...read more
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216AF647 (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216APC (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216PE (blue) and a matched isotype control (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
10B1.3E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SLC34A1 Antibody (10B1.3E9) Summary

Immunogen
Two synthetic peptides made to an internal portions of the human SLC34A1 protein (between amino acids 25-150 and 100-200) [UniProt Q06495]
Localization
Membrane-Cytoplasmic
Marker
Tubulointerstitium Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SLC34A1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
SLC34A1 is a 639 amino acids long protein with predicted molecular weight of 68.9 kDa, however, because of glycosylation, the processed protein may show up at higher than predicted molecular weight position on Western blot.
Theoretical MW
68.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
SLC34A1 Overexpression Lysate
Publications
Read Publications using NBP2-42216.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for SLC34A1 Antibody (10B1.3E9)

  • FRTS2
  • Na(+)/Pi cotransporter 2A
  • Na(+)-dependent phosphate cotransporter 2A
  • naPi-2a
  • NAPI-3
  • NPT2NPHLOP1
  • NPTIIa
  • renal sodium-dependent phosphate transporter
  • SLC11
  • SLC17A2Na+-phosphate cotransporter type II
  • Sodium/phosphate cotransporter 2A
  • sodium/phosphate co-transporter
  • sodium-dependent phosphate transport protein 2A
  • Sodium-phosphate transport protein 2A
  • solute carrier family 17 (sodium phosphate), member 2
  • solute carrier family 34 (sodium phosphate), member 1
  • Solute carrier family 34 member 1

Background

SLC34A1 / NaPi-IIa [Solute carrier family 34 (type II sodium/phosphate cotransporter), member 1] belongs to the type II sodium-phosphate cotransporter family and is a multi-pass membrane protein critically involved in the maintenance of renal inorganic phosphate (Pi) homeostasis and the plasma Pi levels. SLC34A1 acts as the most significant regulator of Pi homeostasis through its reabsorption via Na(+) cotransport in the brush border membrane of renal tissues. It is mainly expressed in the kidney wherein it accounts for 70-80% of the apical influx, however, its presence in some other tissues such as lungs, testes, fallopian tubes, and heart myocytes has also been documented (UniProt and Human Protein Atlas project databases). Signaling implicated in SLC34A1's regulation include the protein kinases A /C, ERK1/2, Klotho/KL, the PI3K/PKB/GSK3 kinase cascade, NHERF1/ SLC9A3R1, AMP-activated protein kinase AMPK, JAK2, and B-RAF. Moreover, SLC34A1 expression has been reported to be controlled by dietary Pi and hormones namely 1,25-dihydroxyvitamin D3, FGF23, Dopamine, Thyroid Hormone, Glucocorticoids, Estrogen /E2 and Triiodothyronine/T3. Besides renal Pi homeostasis, SLC34A1 has been suggested to be involved in several biological processes such as kidney development, bone remodeling, dentinogenesis, cholesterol metabolism, cellular response to metal ion/drug, parathyroid hormone stimulus, protein homooligomerization etc. SLC34A1 mutations have been shown to be associated with diseases such as Nephrolithiasis/osteoporosis, hypophosphatemic, 1 (NPHLOP1), Fanconi renotubular syndrome 2 (FRTS2) and idiopathic infantile hypercalcemia (IIH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC34A1 Antibody (NBP2-42216)(2)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC34A1 Antibody (NBP2-42216) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-42216AF350
Alexa Fluor 405 NBP2-42216AF405
Alexa Fluor 488 NBP2-42216AF488
Alexa Fluor 532 NBP2-42216AF532
Alexa Fluor 594 NBP2-42216AF594
Alexa Fluor 647 NBP2-42216AF647
Alexa Fluor 700 NBP2-42216AF700
Alexa Fluor 750 NBP2-42216AF750
Allophycocyanin NBP2-42216APC
Allophycocyanin/Cy7 NBP2-42216APCCY7
Biotin NBP2-42216B
DyLight 350 NBP2-42216UV
DyLight 405 NBP2-42216V
DyLight 488 NBP2-42216G
DyLight 550 NBP2-42216R
DyLight 594 NBP2-42216DL594
DyLight 650 NBP2-42216C
DyLight 680 NBP2-42216FR
DyLight 755 NBP2-42216IR
FITC NBP2-42216F
HRP NBP2-42216H
Janelia Fluor 549 NBP2-42216JF549
Janelia Fluor 646 NBP2-42216JF646
PE NBP2-42216PE
PE/Atto594 NBP2-42216PEATT594
PE/Cy5.5 NBP2-42216PECY55
PE/Cy7 NBP2-42216PECY7
PerCP NBP2-42216PCP

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Blogs on SLC34A1.

SLC34A1 - major regulator of inorganic phosphate (Pi) homeostasis
SLC34A1 encodes the 69 kDa sodium-dependent phosphate transport protein 2A (Npt2a). SLC34A is a member of the type II sodium-phosphate co-transporter family, along with SLC34A3 which encodes Npt2c. These proteins are abundantly expressed along the...  Read full blog post.

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Bioinformatics

Gene Symbol SLC34A1
Entrez
Uniprot