PDZK1 Antibody (SR2158) - BSA Free

Western Blot: PDZK1 Antibody (SR2158) [NBP3-22197] - Western blot analysis using NBP3-22197 in T47-D cell lysate.

• Reactivity: Hu
• Applications: WB, IP
• Clone: SR2158
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PDZK1 Antibody (SR2158) - BSA Free Summary

• Description: Novus Biologicals Rabbit PDZK1 Antibody (SR2158) - BSA Free (NBP3-22197) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human PDZK1 (Uniprot #: Q5T2W1)
• Specificity: Detects endogenous levels of total PDZK1
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDZK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PDZK1 Antibody (SR2158) - BSA Free

• CAP70
• CFTR-associated protein of 70 kDa
• CLAMP
• Na(+)/H(+) exchanger regulatory factor 3
• Na/Pi cotransporter C-terminal-associated protein 1
• NaPi-Cap1
• NHERF3
• NHERF-3
• NHERF3PDZD1Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• PDZ domain containing 1
• PDZ domain-containing protein 1
• PDZ-containing kidney protein 1
• PDZD1
• PDZK1
• Sodium-hydrogen exchanger regulatory factor 3

Background

PDZK1 is a scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. It may be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, PDZK1 may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. It may also play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. PDZK1 may potentiate the CFTR chloride channel activity. PDZK1 functions to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism and may be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport, therefore playing an important role in tubule function .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDZK1