PDX-1/IPF1 Antibody [Biotin]

PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to pancreatic progenitor cells using Goat Anti-Human PDX-1/IPF1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

PDX-1/IPF1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human PDX‑1/IPF1
  Ala91-Arg283
  Accession # P52945
• Specificity: Detects human PDX‑1/IPF1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDX1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody [Biotin]

• Glucose-sensitive factor
• IDX1
• IDX-1GSF
• Insulin promoter factor 1
• insulin promoter factor 1, homeodomain transcription factor
• Insulin upstream factor 1
• IPF1
• IPF1pancreas/duodenum homeobox protein 1
• Islet/duodenum homeobox-1
• MODY4IUF1
• pancreatic and duodenal homeobox 1
• pancreatic-duodenal homeobox factor 1
• PDX-1
• PDX-1IPF-1
• somatostatin transcription factor 1
• Somatostatin-transactivating factor 1
• STF-1IUF-1

Background

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDX-1/IPF1 Antibody (BAF2419)(3)

Publications using BAF2419

• Leavens, KF;Osorio-Quintero, C;Yeuteuh, EA;Perez-Profeta, FT;Dattoli, AA;Cardenas-Diaz, FL;French, DL;Gadue, P; Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background Stem cell research 2024-09-11 [PMID: 39276530] (Flow Cytometry, Human)
• S Kishore, E De Franco, FL Cardenas-D, LR Letourneau, M Sanyoura, C Osorio-Qui, DL French, SAW Greeley, AT Hattersley, P Gadue A Non-Coding Disease Modifier of Pancreatic Agenesis Identified by Genetic Correction in a Patient-Derived iPSC Line Cell Stem Cell, 2020-05-21;0(0):. 5/21/2020 [PMID: 32442395]
• S. M. Soltani, T. D. O’Brien, G. Loganathan, M. D. Bellin, T. Anazawa, M. Tiwari et al. Severely fibrotic pancreases from young patients with chronic pancreatitis: evidence for a ductal origin of islet neogenesis Acta Diabetologica 2013-10-01 [PMID: 21773756] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: PDX1
• Uniprot:
  • P52945()