Glut2 Antibody

Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glut2 in total lysates of MIN6 cells using anti-Glut2 (NBP2-22218) antibody at 1/500 dilution. Image was submitted via customer review.

Immunocytochemistry/Immunofluorescence: Glut2 Antibody [NBP2-22218] - Glut2 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-WT mice; (ii, v) and (iii,vi) reduction of Glut2 and insulin expression in STZ-WT mice compared to control mice. B. ( Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0105550), licensed under a CC-BY licence.

Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glucose Transporter GLUT2 in human pancreas.

Immunohistochemistry: Glut2 Antibody [NBP2-22218] - Staining of Glucose Transporter GLUT2 in mouse liver.

Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-K-RasV12 mice; (ii, v) and (iii,vi) reduction of Glut2 and insulin expression in STZ-K-RasV12 mice compared to untreated K-RasV12 control mice. Magnification 200x. Calibration bar: 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0105550), licensed under a CC-BY licence.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Glut2 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human GLUT2 protein (between residues 50-150) [UniProt# P11168]
• Localization: Integral membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC2A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1.0 - 2.0 ug/ml
• Application Notes: This Glucose Transporter GLUT2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 55 kDa. In ICC/IF membrane staining was observed in HepG2 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control:

  Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  Glut2 Peptide (NBP2-22218PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glut2 Antibody

• GLUT2 Glucose transporter type 2, liver
• Glut2
• GLUT-2
• SLC2A2
• solute carrier family 2 (facilitated glucose transporter), member 2
• solute carrier family 2, facilitated glucose transporter member 2

Background

SLC2A2 (Solute Carrier Family 2), also known as Glucose Transporter GLUT2, is an integral plasma membrane glycoprotein found in the liver, islet beta cells, intestine, and kidney epithelium. This isoform of the glucose transporter mediates the two way transfer of glucose across the plasma membrane and is responsible for the uptake of glucose in beta cells. Mutations in SLC2A2 lead to Fanconi-Bickel syndrome (FBS), which results in hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose. Recent studies have shown that mutations in SLC2A2 can cause neonatal diabetes, and therefore may contribute to human insulin secretion (PMID: 22660720). Novel SLC2A2 mutations have also been discovered and are being investigated to determine their roles in FBS as well (PMID: 22145468).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut2 Antibody (NBP2-22218)(8)

Publications using NBP2-22218

• Shriwas P, Roberts D, Li Y et al. A small-molecule pan-class I glucose transporter inhibitor reduces cancer cell proliferation in vitro and tumor growth in vivo by targeting glucose-based metabolism Cancer & metabolism Mar 26 2021 [PMID: 33771231] (IHC)
• Bernard H, Teijeiro A, Chaves-Perez A et al. Coxsackievirus B Type 4 Infection in beta Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing Cell Reports Medicine Oct 1 2020 [PMID: 33205075] (WB, Mouse)
• Lei Y, Hu Q, Gu J Expressions of Carbohydrate Response Element Binding Protein and Glucose Transporters in Liver Cancer and Clinical Significance Pathol. Oncol. Res. Aug 12 2019 [PMID: 31407220] (IHC-P, Human)
• Nakagawa, K;Fujiwara, K;Nishimura, A;Murakami, C;Kawamoto, K;Ichinose, C;Kunitou, Y;Suhara, Y;Okano, T;Hasegawa, H; UBIAD1 Plays an Essential Role in the Survival of Pancreatic Acinar Cells Int J Mol Sci Apr 22 2019 [PMID: 31013667] (IHC, Mouse)
• Du J, Hu C, Bai J et al. Intestinal Glucose Absorption Was Reduced by Vertical Sleeve Gastrectomy via Decreased Gastric Leptin Secretion Obes Surg Jun 18 2018 [PMID: 29915972] (Mouse)
• Hassani-Nezhad-Gashti F, Rysa J, Kummu O et al. Activation of nuclear receptor PXR impairs glucose tolerance and dysregulates GLUT2 expression and subcellular localization in liver Biochem. Pharmacol. 2018 Jan 05 [PMID: 29309761] (Mouse)
• Kim DS, Song L, Wang J et al. GRP94 is an Essential Regulator of Pancreatic B Cell Development, Mass and Function in Male Mice. Endocrinology 2017 Dec 20 [PMID: 29272356] (Mouse)
• Micucci C, Orciari S, Catalano A. Hyperglycemia Promotes K-Ras-Induced Lung Tumorigenesis through BASCs Amplification PLoS ONE 2014 Aug 22 [PMID: 25144301]

Reviews for Glut2 Antibody (NBP2-22218) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer WB Human 01/13/2020

Summary

• Application: Western Blot
• Sample Tested: Cultured cancer cell lysates
• Species: Human
• Lot: B-1

reviewed by: Kohsuke Kataoka WB Mouse 02/07/2018

Summary

• Application: Western Blot
• Sample Tested: Min6 mouse insulinoma whole cell lysate
• Species: Mouse
• Lot: A-2

Bioinformatics

• Gene Symbol: SLC2A2
• Entrez:
  • 6514(Human)
  • 20526(Mouse)
• Uniprot:
  • P11168()
  • P14246()