Glut2 Antibody - BSA Free

Images

 
Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glut2 in total lysates of MIN6 cells using anti-Glut2 (NBP2-22218) antibody at 1/500 dilution. Image was submitted via customer review.
Immunocytochemistry/ Immunofluorescence: Glut2 Antibody [NBP2-22218] - Glut2 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-WT mice; (ii, v) and ...read more
Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glucose Transporter GLUT2 in human pancreas.
Immunohistochemistry: Glut2 Antibody [NBP2-22218] - Staining of Glucose Transporter GLUT2 in mouse liver.
Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-K-RasV12 mice; (ii, v) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Glut2 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human GLUT2 protein (between residues 50-150) [UniProt# P11168]
Localization
Integral membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1.0 - 2.0 ug/ml
Application Notes
This Glucose Transporter GLUT2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 55 kDa. In ICC/IF membrane staining was observed in HepG2 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control
Human Pancreas Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
Glut2 Peptide (NBP2-22218PEP)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NBP2-22218 in the following applications:

Publications
Read Publications using
NBP2-22218 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glut2 Antibody - BSA Free

  • GLUT2 Glucose transporter type 2, liver
  • Glut2
  • GLUT-2
  • SLC2A2
  • solute carrier family 2 (facilitated glucose transporter), member 2
  • solute carrier family 2, facilitated glucose transporter member 2

Background

SLC2A2 (Solute Carrier Family 2), also known as Glucose Transporter GLUT2, is an integral plasma membrane glycoprotein found in the liver, islet beta cells, intestine, and kidney epithelium. This isoform of the glucose transporter mediates the two way transfer of glucose across the plasma membrane and is responsible for the uptake of glucose in beta cells. Mutations in SLC2A2 lead to Fanconi-Bickel syndrome (FBS), which results in hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose. Recent studies have shown that mutations in SLC2A2 can cause neonatal diabetes, and therefore may contribute to human insulin secretion (PMID: 22660720). Novel SLC2A2 mutations have also been discovered and are being investigated to determine their roles in FBS as well (PMID: 22145468).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut2 Antibody (NBP2-22218)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: IF/IHC, IHC-Fr, IHC-FrFl, IHC-P, WB.


Filter By Application
IF/IHC
(2)
IHC-Fr
(1)
IHC-FrFl
(1)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(10)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP2-22218 Applications Species
Juras J, Webb M, Young L et al. In situ microwave fixation provides an instantaneous snapshot of the brain metabolome Cell Reports Methods 2023-04-01 [PMID: 37159672] (IHC-FrFl, Mouse) IHC-FrFl Mouse
Shiraki A, Oyama JI, Shimizu T, Node K Linagliptin exacerbates heart failure due to energy deficiency via downregulation of glucose utilization and absorption in a mouse model European journal of pharmacology 2023-03-23 [PMID: 36965743]
Yang K, Sun J, Zhang Z et al. Reduction of mRNA m6A associates with glucose metabolism via YTHDC1 in human and mice Diabetes research and clinical practice 2023-03-04 [PMID: 36878322] (WB, Mouse) WB Mouse
Cornell D, Miwa S, Georgiou M et al. Pseudoislet Aggregation of Pancreatic beta-Cells Improves Glucose Stimulated Insulin Secretion by Altering Glucose Metabolism and Increasing ATP Production Cells 2022-07-29 [PMID: 35954174] (WB, Mouse) WB Mouse
Liu R, Liu C, He X et al. MicroRNA-21 promotes pancreatic beta cell function through modulating glucose uptake Nature communications 2022-06-21 [PMID: 35729232] (WB, Mouse) WB Mouse
Visavadiya NP, Rossiter HB, Khamoui AV. Distinct glycolytic pathway regulation in liver, tumour and skeletal muscle of mice with cancer cachexia Cell Biochemistry and Function 2021-08-01 [PMID: 34129243]
Shriwas P, Roberts D, Li Y et al. A small-molecule pan-class I glucose transporter inhibitor reduces cancer cell proliferation in vitro and tumor growth in vivo by targeting glucose-based metabolism Cancer & metabolism 2021-03-26 [PMID: 33771231] (IF/IHC) IF/IHC
Bernard H, Teijeiro A, Chaves-Perez A et al. Coxsackievirus B Type 4 Infection in beta Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing Cell Reports Medicine 2020-10-01 [PMID: 33205075] (WB, Mouse) WB Mouse
Lei Y, Hu Q, Gu J Expressions of Carbohydrate Response Element Binding Protein and Glucose Transporters in Liver Cancer and Clinical Significance Pathol. Oncol. Res. 2019-08-12 [PMID: 31407220] (IHC-P, Human) IHC-P Human
Nakagawa, K;Fujiwara, K;Nishimura, A;Murakami, C;Kawamoto, K;Ichinose, C;Kunitou, Y;Suhara, Y;Okano, T;Hasegawa, H; UBIAD1 Plays an Essential Role in the Survival of Pancreatic Acinar Cells Int J Mol Sci 2019-04-22 [PMID: 31013667] (IF/IHC, Mouse) IF/IHC Mouse
Show All 15 Publications.

Reviews for Glut2 Antibody (NBP2-22218) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-22218:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Glut2 NBP2-22218
Enlarge
5
reviewed by:
Zeyuan Zhang
WB Mouse 07/31/2023
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesMouse
LotB-5
Western Blot Glut2 NBP2-22218
Enlarge
4
reviewed by:
Verified Customer
WB Human 01/13/2020
View

Summary

ApplicationWestern Blot
Sample TestedCultured cancer cell lysates
SpeciesHuman
LotB-1
Western Blot Glut2 NBP2-22218
Enlarge
4
reviewed by:
Kohsuke Kataoka
WB Mouse 02/07/2018
View

Summary

ApplicationWestern Blot
Sample TestedMin6 mouse insulinoma whole cell lysate
SpeciesMouse
LotA-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glut2 Antibody (NBP2-22218) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Glut2 Products

Research Areas for Glut2 Antibody (NBP2-22218)

Find related products by research area.

Blogs on Glut2.

Deficiency of GluT1 leads to neurological problems while excess is involved in cancers
By Jamshed Arslan, Pharm. D., PhD. What Are GluTs?Mammalian cell metabolism is incomplete without glucose   . Glucose is a monosaccharide that is transported to the cells through facilitative diffusion, a ...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Zeyuan Zhang
07/31/2023
Application: WB
Species: Mouse

Verified Customer
01/13/2020
Application: WB
Species: Human

Kohsuke Kataoka
02/07/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SLC2A2
Entrez
Uniprot