Glut2 Antibody

Images

 
Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glut2 in total lysates of MIN6 cells using anti-Glut2 (NBP2-22218) antibody at 1/500 dilution. Image was submitted via customer review.
Immunocytochemistry/ Immunofluorescence: Glut2 Antibody [NBP2-22218] - Glut2 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-WT mice; (ii, v) and ...read more
Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glucose Transporter GLUT2 in human pancreas.
Immunohistochemistry: Glut2 Antibody [NBP2-22218] - Staining of Glucose Transporter GLUT2 in mouse liver.
Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-K-RasV12 mice; (ii, v) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Glut2 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human GLUT2 protein (between residues 50-150) [UniProt# P11168]
Localization
Integral membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 - 2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This Glucose Transporter GLUT2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 55 kDa. In ICC/IF membrane staining was observed in HepG2 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control
Human Pancreas Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-22218 in the following applications:

Publications
Read Publications using
NBP2-22218 in the following applications:

Reactivity Notes

Immunogen sequence has 78% homology to bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glut2 Antibody

  • GLUT2 Glucose transporter type 2, liver
  • Glut2
  • GLUT-2
  • SLC2A2
  • solute carrier family 2 (facilitated glucose transporter), member 2
  • solute carrier family 2, facilitated glucose transporter member 2

Background

SLC2A2 (Solute Carrier Family 2), also known as Glucose Transporter GLUT2, is an integral plasma membrane glycoprotein found in the liver, islet beta cells, intestine, and kidney epithelium. This isoform of the glucose transporter mediates the two way transfer of glucose across the plasma membrane and is responsible for the uptake of glucose in beta cells. Mutations in SLC2A2 lead to Fanconi-Bickel syndrome (FBS), which results in hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose. Recent studies have shown that mutations in SLC2A2 can cause neonatal diabetes, and therefore may contribute to human insulin secretion (PMID: 22660720). Novel SLC2A2 mutations have also been discovered and are being investigated to determine their roles in FBS as well (PMID: 22145468).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut2 Antibody (NBP2-22218)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, IHC-P.


Filter By Application
IHC
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
All Species

Reviews for Glut2 Antibody (NBP2-22218) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-22218:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Glut2 NBP2-22218
Enlarge
reviewed by:
Anonymous
WB Human 01/13/2020
View

Summary

ApplicationWestern Blot
Sample TestedCultured cancer cell lysates
SpeciesHuman
LotB-1
Western Blot Glut2 NBP2-22218
Enlarge
reviewed by:
Kohsuke Kataoka
WB Mouse 02/07/2018
View

Summary

ApplicationWestern Blot
Sample TestedMin6 mouse insulinoma whole cell lysate
SpeciesMouse
LotA-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glut2 Antibody (NBP2-22218) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22218AF350
Alexa Fluor 405 NBP2-22218AF405
Alexa Fluor 488 NBP2-22218AF488
Alexa Fluor 532 NBP2-22218AF532
Alexa Fluor 594 NBP2-22218AF594
Alexa Fluor 647 NBP2-22218AF647
Alexa Fluor 700 NBP2-22218AF700
Alexa Fluor 750 NBP2-22218AF750
Biotin NBP2-22218B
DyLight 350 NBP2-22218UV
DyLight 405 NBP2-22218V
DyLight 488 NBP2-22218G
DyLight 550 NBP2-22218R
DyLight 594 NBP2-22218DL594
DyLight 650 NBP2-22218C
DyLight 680 NBP2-22218FR
DyLight 755 NBP2-22218IR
FITC NBP2-22218F
HRP NBP2-22218H
Janelia Fluor 549 NBP2-22218JF549
Janelia Fluor 646 NBP2-22218JF646

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Discover related pathways, diseases and genes to Glut2 Antibody (NBP2-22218). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Glut2

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Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
01/13/2020
Application: WB
Species: Human

Kohsuke Kataoka
02/07/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SLC2A2
Entrez
Uniprot
COVID-19 update