Cytokeratin 19 Antibody

Images

 
Western Blot: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in 1. HepG2 and 2. MCF7 lysates.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - MCF7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Staining of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.
Simple Western: Cytokeratin 19 Antibody [NB100-687] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing A246conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.08 mg/ml

Cytokeratin 19 Antibody Summary

Immunogen
Synthetic peptide derived from the C-terminal region of human Cytokeratin 19 (between residues 350-400) [UniProt P08727]
Localization
Cytoplasmic.
Marker
Epithelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT19
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
Application Notes
This Cytokeratin 19 antibody is useful for Western Blot where a band is seen ~44 kDa, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 3
using
NB100-687 in the following applications:

Publications
Read Publications using
NB100-687 in the following applications:

  • 2 publications
  • IHC
    2 publications
  • WB
    1 publication

Reactivity Notes

Human, mouse, and monkey. Rat reactivity reported in scientific literature (PMID: 25401473)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.08 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 19 Antibody

  • CK19
  • CK-19
  • Cytokeratin 19
  • Cytokeratin-19,40-kDa keratin intermediate filament
  • EndoC
  • K19
  • K19cytokeratin-19
  • K1CS
  • keratin 19
  • keratin, type I cytoskeletal 19
  • keratin, type I, 40-kd
  • keratin-19
  • Krt19
  • MGC15366

Background

Cytokeratin 19 (KRT19) is an intermediate filament (IF) protein that is part of the Keratin family of proteins. This protein is also known by the names of Keratin 19 or Keratin type I Cytoskeletal 19. The Keratin family consists of Keratin 1-20, which are divided into two subtypes: Type 1 contains the acidic Cytokeratin proteins consisting of Cytokeratins 9-20, including KRT19; Type 2 Keratins include the basic to neutral protein and contains Cytokeratin 1-8. Type 1 and Type 2 Keratins often form oligomers, interacting to create heterotypic chains. However, KRT19 is unique in that while being the smallest of the Type 1 Keratin proteins, it does not interact with any Type 2 Keratins. IF proteins such as KRT19 are integral for defining and maintaining cellular structure and morphology, and are important for cellular integrity by anchoring to membrane complexes via proteins such as Epiplakin, Trichoplein, Periplakin, Plakophilins, Plectin, BPAG1, Desmoplakin, and Envoplakin. KRT19 is also involved in the organization of the myofibers of striated muscle. KRT19 and other Cytokeratin proteins are mainly found in epithelial cells, with KRT19 being expressed exclusively in defined zones of basal keratinocytes. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 19 Antibody (NB100-687)(4)

Review for Cytokeratin 19 Antibody (NB100-687) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-687:
Filter by Applications
IHC-P
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Mouse 03/04/2009
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Tissue Sections - Liver
SpeciesMouse
Lot1174
Commentssome background

Blocking

Blocking DetailsBlocking Buffer: normal goat serum, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/50, Incubation Dilution Buffer: PBS, Incubation Time: 18 hours, Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-rabbit IgG

Details

Detection Notess

Comments

Commentssome background

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 19 Antibody (NB100-687). (Showing 1 - 1 of 1 FAQs).

  1. I would like to use CK-19 Antibody (Cat # NB100-687) for bile duct/liver carcinoma evaluation. Can I use this antibody for IHC staining of rat bile duct? Please provide reference for this CK-19 antibody for the IHC-Papplication for Cholangiocarcinoma in rats, if possible.
    • Our antibody NB100-687 is validated for IHC detection of the rat CK19 protein. We have one publication that has used our antibody with rat HSC: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4348953/ I was also able to find some other publications that show that CK19 protein can be detected in cholangiocarcinoma sections: http://onlinelibrary.wiley.com/doi/10.1002/path.1711560405/abstracthttp://onlinelibrary.wiley.com/doi/10.1046/j.1365-2168.1998.00815.x/abstracthttp://link.springer.com/article/10.1245/s10434-007-9650-y

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-687AF405
Alexa Fluor 488 Labeled NB100-687AF488
Alexa Fluor 647 Labeled NB100-687AF647
Alexa Fluor 700 Labeled NB100-687AF700
Biotin Labeled NB100-687B
DyLight 405 Labeled NB100-687V
DyLight 488 Labeled NB100-687G
DyLight 550 Labeled NB100-687R
DyLight 650 Labeled NB100-687C
FITC Labeled NB100-687F
HRP Labeled NB100-687H

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Recent Reviews

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Anonymous
03/04/2009
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol KRT19
Entrez
Uniprot