Cytokeratin 19 Antibody - BSA Free

Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.

Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep13320), licensed under a CC-BY licence.

Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Inhibition of MST improves liver regeneration in aged mice. Representative photomicrograph of an aged liver treated with siMST and stained with H&E and by IHC for CK19 and Ki67. Arrows indicate ductal regions with no signs of reaction or oval cell expansion. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in MCF7 cells using Cytokeratin 19 antibody (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Flow Cytometry: Cytokeratin 19 Antibody [NB100-687] - An intracellular stain was performed on MCF7 cells with Cytokeratin 19 Antibody NB100-687AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in 1) HepG2 and 2) MCF7 lysates.

Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Staining of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Rt, Pm-Or
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Cytokeratin 19 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide derived from the C-terminal region of human Cytokeratin 19 (between residues 350-400) [UniProt P08727]
• Localization: Cytoplasmic.
• Marker: Epithelial Cell Marker
• Predicted Species: Orangutan (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT19
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2-5 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Simple Western 1:1000
  • Western Blot 1:1000
• Application Notes: This Cytokeratin 19 antibody is useful for Western Blot where a band is seen ~44 kDa, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25401473)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytokeratin 19 Antibody - BSA Free

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Background

Cytokeratin 19 (KRT19) is an intermediate filament (IF) protein that is part of the Keratin family of proteins. This protein is also known by the names of Keratin 19 or Keratin type I Cytoskeletal 19. The Keratin family consists of Keratin 1-20, which are divided into two subtypes: Type 1 contains the acidic Cytokeratin proteins consisting of Cytokeratins 9-20, including KRT19; Type 2 Keratins include the basic to neutral protein and contains Cytokeratin 1-8. Type 1 and Type 2 Keratins often form oligomers, interacting to create heterotypic chains. However, KRT19 is unique in that while being the smallest of the Type 1 Keratin proteins, it does not interact with any Type 2 Keratins. IF proteins such as KRT19 are integral for defining and maintaining cellular structure and morphology, and are important for cellular integrity by anchoring to membrane complexes via proteins such as Epiplakin, Trichoplein, Periplakin, Plakophilins, Plectin, BPAG1, Desmoplakin, and Envoplakin. KRT19 is also involved in the organization of the myofibers of striated muscle. KRT19 and other Cytokeratin proteins are mainly found in epithelial cells, with KRT19 being expressed exclusively in defined zones of basal keratinocytes. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 19 Antibody (NB100-687)(7)

Publications using NB100-687

• Barthet VJA, Brucoli M, Ladds MJGW et al. Autophagy suppresses the formation of hepatocyte-derived cancer-initiating ductular progenitor cells in the liver Science advances 2021-06-01 [PMID: 34088666] (IHC-P, Mouse)
• OrrU C, Perra A, Kowalik MA et al. Distinct Mechanisms Are Responsible for Nrf2-Keap1 Pathway Activation at Different Stages of Rat Hepatocarcinogenesis Cancers (Basel) 2020-08-16 [PMID: 32824383] (IF/IHC, Rat)
• Kim N, Kim HK, Lee K et al. Single-cell RNA sequencing demonstrates the molecular and cellular reprogramming of metastatic lung adenocarcinoma Nat Commun 2020-05-08 [PMID: 32385277] (IHC-P, Human)
• Loforese G, Malinka T, Keogh A et al. Impaired liver regeneration in aged mice can be rescued by silencing Hippo core kinases MST1 and MST2. EMBO Mol Med. 2016-12-09 [PMID: 27940445] (ICC/IF, WB, Mouse)
• Sawitza I, Kordes C, Gotze S et al. Bile acids induce hepatic differentiation of mesenchymal stem cells. Sci Rep 2015-08-25 [PMID: 26304833] (ICC/IF, Rat)
• Kordes C, Sawitza I, Gotze S et al. Hepatic stellate cells contribute to progenitor cells and liver regeneration. J. Clin. Invest. 2014-12-01 [PMID: 25401473] (IF/IHC, Rat)
• Takeda K, Kojima Y, Ikejima K et al. Death receptor 5 mediated-apoptosis contributes to cholestatic liver disease. PNAS;105(31):10895-10900. 2008-01-01 [PMID: 18667695] (IF/IHC, Mouse)

Review for Cytokeratin 19 Antibody (NB100-687) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: System Admin IHC-P Mouse 03/04/2009

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Tissue Sections - Liver
• Species: Mouse
• Lot: 1174

FAQs for Cytokeratin 19 Antibody (NB100-687). (Showing 1 - 1 of 1 FAQs).

1. I would like to use CK-19 Antibody (Cat # NB100-687) for bile duct/liver carcinoma evaluation. Can I use this antibody for IHC staining of rat bile duct? Please provide reference for this CK-19 antibody for the IHC-Papplication for Cholangiocarcinoma in rats, if possible.
   • Our antibody NB100-687 is validated for IHC detection of the rat CK19 protein. We have one publication that has used our antibody with rat HSC: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4348953/ I was also able to find some other publications that show that CK19 protein can be detected in cholangiocarcinoma sections: http://onlinelibrary.wiley.com/doi/10.1002/path.1711560405/abstracthttp://onlinelibrary.wiley.com/doi/10.1046/j.1365-2168.1998.00815.x/abstracthttp://link.springer.com/article/10.1245/s10434-007-9650-y

Bioinformatics

• Gene Symbol: KRT19
• Entrez:
  • 3880(Human)
  • 16669(Mouse)
  • 360626(Rat)
• Uniprot:
  • P08727()