Glucokinase/GCK Antibody (OTI3E3)

Images

 
Western Blot: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCK.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Human prostate tissue.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GCK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GCK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GCK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GCK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of paraffin-embedded Human breast tissue using anti-GCK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of paraffin-embedded Human Kidney tissue using anti-GCK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of paraffin-embedded Human lung tissue using anti-GCK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of paraffin-embedded Human pancreas tissue using anti-GCK mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI3E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Glucokinase/GCK Antibody (OTI3E3) Summary

Immunogen
Full length human recombinant protein of human GCK (NP_000153) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GCK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glucokinase/GCK Antibody (OTI3E3)

  • EC 2.7.1
  • EC 2.7.1.2
  • FGQTL3
  • GCK
  • GK
  • GLK
  • glucokinase (hexokinase 4)
  • Glucokinase
  • Hexokinase-4
  • Hexokinase-D
  • HHF3
  • HK4
  • HK4pancreatic isozyme
  • HKIV
  • HXKP
  • LGLK
  • maturity onset diabetes of the young 2
  • MODY2

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GCK
Entrez