MafA Antibody

Images

 
Western Blot: MafA Antibody [NB400-137] - Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Affinity purified anti-MafA antibody (NB400-137), anti-MafB antibody ...read more
Immunohistochemistry-Paraffin: MafA Antibody [NB400-137] - Immunohistochemical staining of MafA (blue) and insulin (green) in mouse pancreas. Image from verified customer review.
Western Blot: MafA Antibody [NB400-137] - Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, ...read more
Gel Super Shift Assays: MafA Antibody [NB400-137] - Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, GS, ICC/IF, IHC, IHC-Fr, IHC-P, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MafA Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 359) of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A using the numbering given in entry NP_919331.1 (GeneID 378435).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAFA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Gel Super Shift Assays 1 to 5 ug/20 ul reaction
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000 to 1:3000
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
A band ~36-40 kDa is seen in WB analysis. Use in IHC-P was reported in the scientific literature (PMID: 22665223). In some cases, the antibody may be diluted further than indicated. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Use in ICC/IF and in IHC-Frozen reported in scientific literature (PMID 23967072). Chip reported in scientific literature (PMID: 26314560).
Reviewed Applications
Read 1 Review rated 5
using
NB400-137 in the following applications:

Publications
Read Publications using
NB400-137 in the following applications:

Reactivity Notes

Human reactivity reported in the scientific literature (PMID: 22665223).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MafA Antibody

  • hMafA
  • Pancreatic beta-cell-specific transcriptional activator
  • RIPE3B1
  • transcription factor MafA
  • Transcription factor RIPE3b1
  • v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
  • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

Background

Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MafA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MafA Antibody (NB400-137)(40)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC-Fr, IHC-P, WB.


Filter By Application
ChIP
(1)
ICC/IF
(3)
IHC-Fr
(1)
IHC-P
(1)
WB
(1)
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Human
(1)
Mouse
(5)
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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NB400-137 Applications Species
Wei Z, Yoshihara E, He N et al. Vitamin D Switches BAF Complexes to Protect B Cells Cell May 17 2018 [PMID: 29754817] (Mouse) Mouse
Sweeney LB, Bikoff JB, Gabitto MI et al. Origin and Segmental Diversity of Spinal Inhibitory Interneurons. Neuron Jan 17 2018 [PMID: 29307712] (ICC/IF, Mouse) ICC/IF Mouse
Akai R, Hosoda A, Yoshino M, Iwawaki T. Constitutive role of GADD34 and CReP in cancellation of phospho-eIF2a-dependent translational attenuation and insulin biosynthesis in pancreatic b cells. Genes Cells 2015 Aug 28 [PMID: 26314560] (ChIP, Mouse) ChIP Mouse
Blanchet E, Van de Velde S, Matsumura S et al. Feedback Inhibition of CREB Signaling Promotes Beta Cell Dysfunction in Insulin Resistance Cell Rep. 2015 Feb 24 [PMID: 25704817] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Alejandro EU, Gregg B, Wallen T et al. Maternal diet-induced microRNAs and mTOR underlie b cell dysfunction in offspring. J Clin. Invest. 2014 Sep 02 [PMID: 25180600]
Francius C, Harris A, Rucchin V et al. Identification of Multiple Subsets of Ventral Interneurons and Differential Distribution along the Rostrocaudal Axis of the Developing Spinal Cord. PLoS One. 2013 Aug 15 [PMID: 23967072] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Campbell-Thompson ML, Heiple T, Montgomery E et al. Staining protocols for human pancreatic islets. J Vis Exp 2012 May [PMID: 22665223] (IHC-P, Human) IHC-P Human
Hu J, Huang T, Li T et al. c-Maf is required for the development of dorsal horn laminae III/IV neurons and mechanoreceptive DRG axon projections. J Neurosci 2012 Apr [PMID: 22514301]
Lausier J, Diaz WC, Roskens V et al. Vagal control of pancreatic beta-cell proliferation. Am J Physiol Endocrinol Metab 2010 Nov [PMID: 20716695]
Artner I, Hang Y, Mazur M et al. MafA and MafB regulate genes critical to beta-cells in a unique temporal manner. Diabetes 2010 Oct [PMID: 20627934]
Show All 40 Publications.

Review for MafA Antibody (NB400-137) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB400-137:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MafA NB400-137
Enlarge
5
reviewed by:
Yves Heremans
IHC-P Mouse 09/21/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MafA Antibody (NB400-137) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-137AF350
Alexa Fluor 405 NB400-137AF405
Alexa Fluor 488 NB400-137AF488
Alexa Fluor 532 NB400-137AF532
Alexa Fluor 594 NB400-137AF594
Alexa Fluor 647 NB400-137AF647
Alexa Fluor 700 NB400-137AF700
Alexa Fluor 750 NB400-137AF750
Biotin NB400-137B
DyLight 350 NB400-137UV
DyLight 405 NB400-137V
DyLight 488 NB400-137G
DyLight 550 NB400-137R
DyLight 594 NB400-137DL594
DyLight 650 NB400-137C
DyLight 680 NB400-137FR
DyLight 755 NB400-137IR
FITC NB400-137F
HRP NB400-137H
Janelia Fluor 549 NB400-137JF549
Janelia Fluor 646 NB400-137JF646

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Yves Heremans
09/21/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol MAFA
Entrez
Uniprot