Pax4 Antibody

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Pax4 Antibody Summary

• Immunogen: E. coli-derived recombinant human Pax4
  Met1-Glu343
  Accession # NP_006184
• Specificity: Detects human Pax4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Pax3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAX4
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax4 Antibody

• KPD
• MGC129960
• MODY9paired box protein Pax-4
• paired box 4
• paired box gene 4
• paired domain gene 4
• Pax4

Background

Pax4 is a member of the paired box family of transcription factors. Members of the Pax family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Pax4 is known to be a key regulator for pancreatic cell differentiation and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax4 Antibody (AF2614)(2)

Publications using AF2614

• Gage B, Baker R, Kieffer T Overexpression of PAX4 reduces glucagon expression in differentiating hESCs. Islets, 2014-01-01;6(2):e29236. 2014-01-01 [PMID: 25483960] (ICC, Human)
• Berneman-Zeitouni D, Molakandov K, Elgart M, Mor E, Fornoni A, Dominguez M, Kerr-Conte J, Ott M, Meivar-Levy I, Ferber S The temporal and hierarchical control of transcription factors-induced liver to pancreas transdifferentiation. PLoS ONE, 2014-02-04;9(2):e87812. 2014-02-04 [PMID: 24504462] (ICC, Human)

Bioinformatics

• Gene Symbol: PAX4
• Uniprot:
  • NP_006184()