HNF1 Antibody

Images

 
Biological Strategies: Western Blot: HNF1 Antibody [NBP1-33596] - Analysis of HNF1a transcriptional activity in hepatocytes following transforming growth factor B (TGFB) treatment. (B) Western blot analysis for ...read more
Immunocytochemistry/ Immunofluorescence: HNF1 Antibody [NBP1-33596] - TGFB overrides HNF1a constitutive expression. Immunofluorescence analysis of hepatocytes transfected with pLCPX-HNF1aMyc (HNF1) or the empty vector ...read more
Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:5000. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - detects HNF1 alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.HNF1 alpha stained by HNF1 alpha antibody ...read more
Western Blot: HNF1 Antibody [NBP1-33596] - Analysis. Mouse tissue extracts (50 Ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000.
Western Blot: HNF1 Antibody [NBP1-33596] - Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HNF1 Antibody [NBP1-33596] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1 alpha antibody [N1N3] diluted at 1:1000. Blue: ...read more
Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - detects HNF1 alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HNF1 alpha stained by HNF1 alpha antibody [N1N3] ...read more
Immunoprecipitation: HNF1 Antibody [NBP1-33596] - Immunoprecipitation of HNF1 alpha protein from HepG2 whole cell extracts using 5 ug of HNF1 alpha antibody [N1N3]. Western blot analysis was performed using HNF1 alpha ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PAGE
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

HNF1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary.
Localization
Nucleus
Specificity
human HNF-1 alpha protein.
Predicted Species
Porcine (99%), Bovine (98%), Chicken (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNF1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • SDS-Page
Application Notes
Use in SDS-Page reported in scientific literature (PMID:31543815).
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-33596 in the following applications:

Reactivity Notes

Xenopus laevis (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for HNF1 Antibody

  • HNF1 homeobox A
  • IDDM20
  • LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor
  • MODY3
  • TCF1hepatic
  • Transcription factor 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89680
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, ChIP
NBP1-89679
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-70822
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, ICC/IF, IHC, IHC-P
AF4186
Species: Hu
Applications: WB, ICC
NBP1-86960
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
AF2400
Species: Hu
Applications: WB, IHC, ChIP, ICC
AF2419
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NBP2-45731
Species: Hu, Ca, Pm
Applications: WB, IHC, IHC-P
NBP1-88584
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-93878
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP2-45354
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-27079
Species: Hu, Ca, Pm
Applications: WB, IHC, IHC-P
NBP1-33596
Species: Hu, Mu, Rt, Po, Bv, Ch
Applications: WB, ICC/IF, IHC, IHC-P, IP, PAGE

Publications for HNF1 Antibody (NBP1-33596)(3)

Reviews for HNF1 Antibody (NBP1-33596) (0)

There are no reviews for HNF1 Antibody (NBP1-33596). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HNF1 Antibody (NBP1-33596). (Showing 1 - of FAQs).

    Secondary Antibodies

     

    Isotype Controls

    Other Available Formats

    Additional HNF1 Products

    Bioinformatics Tool for HNF1 Antibody (NBP1-33596)

    Discover related pathways, diseases and genes to HNF1 Antibody (NBP1-33596). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
    Visit Tool

    Diseases for HNF1 Antibody (NBP1-33596)

    Discover more about diseases related to HNF1 Antibody (NBP1-33596).
     

    Pathways for HNF1 Antibody (NBP1-33596)

    View related products by pathway.

    PTMs for HNF1 Antibody (NBP1-33596)

    Learn more about PTMs related to HNF1 Antibody (NBP1-33596).
     

    Research Areas for HNF1 Antibody (NBP1-33596)

    Find related products by research area.

    Blogs on HNF1

    There are no specific blogs for HNF1, but you can read our latest blog posts.

    Customers Who Bought This Also Bought

    Contact Information

    Product PDFs

    Calculators

    Concentration Calculator

    The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

    =
    ÷

    Review this Product

    Be the first to review our HNF1 Antibody and receive a gift card or discount.

    Bioinformatics

    Gene Symbol HNF1A
    Entrez
    Uniprot