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HNF1 Antibody

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Biological Strategies: Western Blot: HNF1 Antibody [NBP1-33596] - Analysis of HNF1a transcriptional activity in hepatocytes following transforming growth factor B (TGFB) treatment. (B) Western blot analysis for ...read more
Immunocytochemistry/ Immunofluorescence: HNF1 Antibody [NBP1-33596] - TGFB overrides HNF1a constitutive expression. Immunofluorescence analysis of hepatocytes transfected with pLCPX-HNF1aMyc (HNF1) or the empty vector ...read more
Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:5000. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - detects HNF1 alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.HNF1 alpha stained by HNF1 alpha antibody ...read more
Western Blot: HNF1 Antibody [NBP1-33596] - Analysis. Mouse tissue extracts (50 Ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000.
Western Blot: HNF1 Antibody [NBP1-33596] - Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HNF1 Antibody [NBP1-33596] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1 alpha antibody [N1N3] diluted at 1:1000. Blue: ...read more
Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - detects HNF1 alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HNF1 alpha stained by HNF1 alpha antibody [N1N3] ...read more
Immunoprecipitation: HNF1 Antibody [NBP1-33596] - Immunoprecipitation of HNF1 alpha protein from HepG2 whole cell extracts using 5 ug of HNF1 alpha antibody [N1N3]. Western blot analysis was performed using HNF1 alpha ...read more
The DNA damage response at telomeres uncapped through TRF2 depletion does not require BRCA1 or CtIPImmortalized Brca1F/− MEFs were infected with retroviruses expressing the indicated shRNAs and/or Cre recombinase, ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IP, PAGE
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

HNF1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary.
Localization
Nucleus
Specificity
human HNF-1 alpha protein.
Predicted Species
Porcine (99%), Bovine (98%), Chicken (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNF1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • SDS-Page
  • Western Blot 1:1000-1:10000
Application Notes
Use in SDS-Page reported in scientific literature (PMID:31543815).
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-33596 in the following applications:

Reactivity Notes

Xenopus laevis (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for HNF1 Antibody

  • HNF1 homeobox A
  • IDDM20
  • LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor
  • MODY3
  • TCF1hepatic
  • Transcription factor 1

Background

HNF1 is a transcription factor required for the expression of several liver-specific genes. The protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HNF1 Antibody (NBP1-33596)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, PAGE, WB.


Filter By Application
ICC/IF
(1)
PAGE
(1)
WB
(6)
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Filter By Species
Human
(3)
Mouse
(2)
Rat
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HNF1 Antibody (NBP1-33596). (Showing 1 - 1 of 1 FAQs).

  1. I would like to know if the HNF1A antibody (NBP1-33596) cross reacts with HNF1B protein. The immunogen for HNF1A is a recombinant fragment corresponding to a region within amino acids 35 and 268 of HNF1 alpha. That particular fragment has a very high homology with HNF1B, in some areas 100%. We have already purchased the HNF1A antibody and conducted experiments using it for immunohistochemistry based on the assumption that it is a specific antibody (as it is stated on the datasheet). I now understand that the specificity of the antibody is uncertain. It is imperative for the interpretation of our data to be certain that the claimed specificity HNF1A antibody is a tested certainty. Since we have spent money (out of a tight budget) and effort on the project we would like to further test the HNF1A antibody cross reactivity with HNF1B. Could you please help us? I could think of two ways. First if you could run cross-reactivity tests, or secondly, if you could provide us with aliquots of the HNF1B antibody and lysates enriched for HNF1A and HNF1B so we could test the specificity in Western blots and immunohistochemistry. Our budget is very tight and we cannot pay for full price only to test the cross-reactivity of the products. It is imperative for our work to exclude the possibility of cross-reaction between HNF1A and HNF1B. In order to do so we need aliquots of the HNF1B antibody and lysates enriched for HNF1A and HNF1B so we could test the specificity in Western blots and immunohistochemistry. Therefore, we do not wish a direct refund or replacement which you kindly offered. Instead, we would like to ask for a generous discount on your three products that we need (HNF1B antibody and lysates enriched for HNF1A and HNF1B) taking into account the situation and our limited budget. In order for the discount to be bigger we would like the quantity from each product to be less than usual since we just want to check the cross-reactivity issue. I would like to have a credit for the cost of the NBP1-33596 antibody, and use it to reduce the cost of the products I would like. You also said that you may be able to get me a discount on top of this.
    • Although we have not tested the cross-reactivity of antibody NBP1-33596 with HNF1B, I have performed a sequence comparison analysis for you, and HNF1A only has 54% homology with HNF1B (56% for isoform B), suggesting that the antibody is unlikely to bind to HNF1B.The website data sheet does state which portion of HNF1 alpha the antibody was raised against, so this information is available for our customers before they begin their experiments. HNF1 alpha was the protein this antibody was generated for, but cross-reactivity against other proteins was not tested. We do not state that the antibody will definitely not bind to HNF1 beta. However, I understand that the datasheet can be misinterpreted. Unfortunately, we are unable to carry out your requests, but we can offer you a refund or replacement. Please let me know how you wish to proceed. I am making enquiries into whether any of our antibodies are known to be specific to either HNF1B or HNF1A, and will let you know as soon as I have an answer. We do have overexpression lysates for HNF1A and HNF1B: NBL-11630 and NBL-11631. Unfortunately, we cannot change the pack size of any of our products. However, we will give you a credit for the cost of the NBP1-33596 antibody, and you can use this to reduce the cost of the products you would like. I may be able to get you a discount on top of this. Unfortunately, none of the HNF1A antibodies have been tested for cross-reactivity to HNF1B, and none of the HNF1B antibodies have been tested for cross-reactivity to HNF1A. Therefore, we do not have any antibodies that I can guarantee are specific to either HNF1A or HNF1B; please accept my apologies. Please could you let me know the PO of your original order with us? Or did you order via a distributor? Also, as I said in my previous email, we do not have an HNF1B antibody that has been tested for cross-reactivity against HNF1A. However, if you would still like one from us, I would recommend NBP1-89680, because the lab have let me know that the antigen of this antibody has 47% sequence identity to the HNF1A gene. This antibody shows nuclear staining of moderate to strong intensity in the gastrointestinal tract, renal tubules, pancreatic ducts and decidual cells; it shows no positivity in hepatocytes. 'Although HNF-1 alpha, but not HNF-1 beta, is expressed in the liver, HNF-1 alpha and HNF-1 beta are coexpressed in the murine Hepa1A cell line and in the mammalian kidney where a subset of hepatocyte genes are expressed', accor

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Bioinformatics

Gene Symbol HNF1A
Entrez
Uniprot