PDGFR alpha Antibody (16A1) [Biotin]

Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) purified / GAM-APC.

Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected 3T3 cells using anti-CD140a (16A1) APC.

Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) PE.

Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) FITC.

Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) biotin / streptavidin-APC.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 16A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: 1 mg/ml

PDGFR alpha Antibody (16A1) [Biotin] Summary

• Description: Store in the dark. Avoid prolonged exposure to light.
• Additional Information: Clone 16A1 was used by HLDA to establish CD designation.
• Immunogen: CD140a-transfected NIH 3T3 cells
• Specificity: The mouse monoclonal antibody 16A1 recognizes CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry 1-2 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
• Theoretical MW: 170 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PDGFR alpha Antibody (16A1) [Biotin]

• alpha-type platelet-derived growth factor receptor
• CD140 antigen-like family member A
• CD140a antigen
• CD140a
• EC 2.7.10
• EC 2.7.10.1
• MGC74795
• PDGF R alpha
• PDGFR alpha
• PDGFR2
• PDGFRA
• PDGFRA/BCR fusion
• PDGF-R-alpha
• platelet-derived growth factor receptor, alpha polypeptide
• rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
• RHEPDGFRA

Background

The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDGFRA